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The map label for this gene is purF [H]

Identifier: 17231143

GI number: 17231143

Start: 4404283

End: 4405782

Strand: Reverse

Name: purF [H]

Synonym: all3651

Alternate gene names: 17231143

Gene position: 4405782-4404283 (Counterclockwise)

Preceding gene: 17231144

Following gene: 17231142

Centisome position: 68.69

GC content: 45.07

Gene sequence:

>1500_bases
ATGATTCCCATTGATTCCGTCACTCCGGATGAGTACCCTAATCAGACTAACAACCCAATTGATAGTCAAGAAAATCGTCC
TGACAAGCCAGAAGAGGCTTGTGGAGTTTTCGGCATTTACGCCCCAGGAGAAAATGTTGCAAAACTGACCTACTTTGGAT
TGTATGCCCTGCAACATCGGGGTCAAGAATCAGCTGGCATTGCCACGTTTGAGGGTACAACAGTTCACCTGCATAAAGAC
ATGGGTTTGGTTTCTCAAGTCTTTAATGAGTCGATTTTAGAAGAGTTGCCTGGTGATATCGGCGTTGGTCATACCCGTTA
CTCTACCACTGGTTCCAGCCGCAAAGTTAATGCCCAGCCTGCTGTCCTAGACACTCGTTTAGGCAAGCTAGCTCTAGCAC
ATAATGGTAATTTAGTCAATACTGTGAAGTTGCGCCAAGAGTTAGTTAATAGCAACTGCAACCTAGTGACAACCACAGAC
TCAGAAATGATTGCTTTTGCGATCGCTCAAGCAGTCAACGCAGGTGCGGACTGGTTAGATGGGGCGATTCAAGCATTCCA
TCGTTGCGAGGGAGCTTTTAGCTTAGTGATTGGCACACCTGTTGGTGTGATGGGAGTGCGTGACACCAACGGCATTCGTC
CTCTGGTAATTGGGATTTTGCCTGGTAATCCTGTGCGCTATGTTTTATCCTCGGAAACTTGTGGTTTAGACATTATTGGC
GCTGAATTTCTACGAGATGTAGAACCAGGTGAAGTAGTTTGGATTACCGAAAAAGGTTTAGCTTCCTACCACTGGAGTCA
AAAGCCAGAACGAAGATTGTGTATTTTCGAGATGATTTATTTTGCTCGTCCCGATAGCCAAATGCACAACGAGAGTTTGT
ACAGTTATCGGATGCGTTTAGGAAGGCAACTAGCAGCCGAATCCTACGTAGATGCTGATATTGTCTTTGGTGTACCTGAT
TCTGGTATTCCTGCTGCTATTGGATTTTCCCAGGCTTCTGGTGTTGCCTACGGTGAAGGGTTAATTAAGAATCGCTATGT
TGGTCGCACCTTTATTCAGCCAACCCAAACCATGCGGGAGTCGGGGATTAAGATGAAACTCAACCCCCTCAAAGATGTGC
TGGCGGGTAAAAGAGTGATTATCGTTGATGACTCCATTGTCAGAGGTACTACTAGCCGCAAACTAGTTAAAGCTTTACGT
GATGCTGGTGCAGCCGAAGTACATATGCGGATTTCTTCCCCACCAGTAACTCATCCTTGTTTCTACGGAATTGATACTGA
TTCTCAAGACCAGCTAATTGCTGCTACTAAGTCAGTAGAAGAGATTGCCAAGCAATTAGAAGTAGAAAGCCTGGCCTATC
TCAGTTGGGATGGGATGTTAGAAGCAACCAGAGAAGACAAAAATAGTTTCTGTTCTGCCTGTTTTACAGGTGATTACCCT
GTTGGTATTCCTGAACAGGTGAAACGCGCTAAGTTGATTTTAGAAAAGGTAGTAGTTTAG

Upstream 100 bases:

>100_bases
TTTGTTAAGACGATTCCGCAACTATCCCTAGACATATTCCCAGTGACCAAATAGTTACGAATTATTTTGACTTGACACCC
CAGCCAGGAGCAAAGCTAGC

Downstream 100 bases:

>100_bases
GGATTAGGAACAAATCAGTGCTGGGTTTTGAGAATTCACTCAGCACTGTCTCAAGCCTACCCAAGCTAAAATAATTAAAT
ATCCTCACAGTGATGGCTTA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHRGQESAGIATFEGTTVHLHKD
MGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQPAVLDTRLGKLALAHNGNLVNTVKLRQELVNSNCNLVTTTD
SEMIAFAIAQAVNAGADWLDGAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGILPGNPVRYVLSSETCGLDIIG
AEFLRDVEPGEVVWITEKGLASYHWSQKPERRLCIFEMIYFARPDSQMHNESLYSYRMRLGRQLAAESYVDADIVFGVPD
SGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQTMRESGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALR
DAGAAEVHMRISSPPVTHPCFYGIDTDSQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
VGIPEQVKRAKLILEKVVV

Sequences:

>Translated_499_residues
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHRGQESAGIATFEGTTVHLHKD
MGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQPAVLDTRLGKLALAHNGNLVNTVKLRQELVNSNCNLVTTTD
SEMIAFAIAQAVNAGADWLDGAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGILPGNPVRYVLSSETCGLDIIG
AEFLRDVEPGEVVWITEKGLASYHWSQKPERRLCIFEMIYFARPDSQMHNESLYSYRMRLGRQLAAESYVDADIVFGVPD
SGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQTMRESGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALR
DAGAAEVHMRISSPPVTHPCFYGIDTDSQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
VGIPEQVKRAKLILEKVVV
>Mature_499_residues
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHRGQESAGIATFEGTTVHLHKD
MGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQPAVLDTRLGKLALAHNGNLVNTVKLRQELVNSNCNLVTTTD
SEMIAFAIAQAVNAGADWLDGAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGILPGNPVRYVLSSETCGLDIIG
AEFLRDVEPGEVVWITEKGLASYHWSQKPERRLCIFEMIYFARPDSQMHNESLYSYRMRLGRQLAAESYVDADIVFGVPD
SGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQTMRESGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALR
DAGAAEVHMRISSPPVTHPCFYGIDTDSQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
VGIPEQVKRAKLILEKVVV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=504, Percent_Identity=40.4761904761905, Blast_Score=364, Evalue=1e-100,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=38.592750533049, Blast_Score=295, Evalue=7e-81,
Organism=Escherichia coli, GI1790167, Length=232, Percent_Identity=29.3103448275862, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=41.701244813278, Blast_Score=335, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6323958, Length=482, Percent_Identity=36.0995850622407, Blast_Score=264, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24659598, Length=518, Percent_Identity=40.1544401544402, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI28573187, Length=491, Percent_Identity=42.9735234215886, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24659604, Length=465, Percent_Identity=42.5806451612903, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54499; Mature: 54499

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHC
GQESAGIATFEGTTVHLHKDMGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQP
CCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEECCCC
AVLDTRLGKLALAHNGNLVNTVKLRQELVNSNCNLVTTTDSEMIAFAIAQAVNAGADWLD
CHHHHHHCEEEEECCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCHHHHH
GAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGILPGNPVRYVLSSETCGLDIIG
HHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEC
AEFLRDVEPGEVVWITEKGLASYHWSQKPERRLCIFEMIYFARPDSQMHNESLYSYRMRL
HHHHCCCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GRQLAAESYVDADIVFGVPDSGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQTMRE
HHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHH
SGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALRDAGAAEVHMRISSPPVTHPC
CCCEEEECHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCE
FYGIDTDSQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHEECCCCC
VGIPEQVKRAKLILEKVVV
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHC
GQESAGIATFEGTTVHLHKDMGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQP
CCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEECCCC
AVLDTRLGKLALAHNGNLVNTVKLRQELVNSNCNLVTTTDSEMIAFAIAQAVNAGADWLD
CHHHHHHCEEEEECCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCHHHHH
GAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGILPGNPVRYVLSSETCGLDIIG
HHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEC
AEFLRDVEPGEVVWITEKGLASYHWSQKPERRLCIFEMIYFARPDSQMHNESLYSYRMRL
HHHHCCCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GRQLAAESYVDADIVFGVPDSGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQTMRE
HHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHH
SGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALRDAGAAEVHMRISSPPVTHPC
CCCEEEECHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCE
FYGIDTDSQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHEECCCCC
VGIPEQVKRAKLILEKVVV
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]