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The map label for this gene is eno [H]

Identifier: 17231030

GI number: 17231030

Start: 4262942

End: 4264231

Strand: Reverse

Name: eno [H]

Synonym: all3538

Alternate gene names: 17231030

Gene position: 4264231-4262942 (Counterclockwise)

Preceding gene: 17231032

Following gene: 17231028

Centisome position: 66.49

GC content: 46.9

Gene sequence:

>1290_bases
ATGAATAATATTGTCGATACAGCCATTGAGGCGATTGTCGCCCGCGAAATTCTCGACTCACGCGGTAGACCAACAATAGA
AGCGGAAGTACATTTATTAAGCGGTGCGGTAGGCTTGGCACAAGTTCCTAGCGGCGCTTCTACAGGCACATTTGAAGCCC
ATGAACTGAGAGACAAGGATAAAAGCCGTTACGGCGGTAAAGGGGTACTCAAGGCTGTACACAACGTTAATGAGATACTA
GCCCCAAAGTTAATAGATTTGGATGCTCTCAACCAAGAACTCATCGACCGGACGATGATTGCCCTTGATGGTTCTGGTAA
CAAATCAAATTTGGGTGCAAATGCGATTTTAGCTGTTTCTTTGGCAGCCGCTAGAGCCGGGGCTGAGTCTTTGGGGATTC
CCCTATATCGTTACTTAGGCGGCCCTTTGGCGAATTTGTTGCCTGTGCCTTTAATGAACGTAATTAATGGTGGGGCGCAC
GCTTCTAATAACGTGGATTTCCAAGAGTTTATGATCGTTCCTGTGGGTGCAACTTCCTTCCGTGAAGCCCTGCGCTGGGG
TGCGGAGGTATTTGCTACCCTCAGTGAAGTGCTGCATGACAAAGGCTTGCTGACTGGTGTAGGCGATGAAGGTGGTTTTG
CACCTAACTTGGAATCTAATCAGGTAGCCTTGGAATTGCTAGTTGCAGCCATTGAAAAAGCAGGATACAAACCAGGGGAA
CAAGTAGCACTGGCGCTAGATGTGGCGGCTAGTGAATTTTACAAGGAAGGGCAGTATGTCTATGATGGTAGACCTCATGC
ACCGACGGAGTTTATCGATTACTTAGGGCAGTTAGTTGATCAATACCCAATTGTGTCCATTGAAGATGGTTTACACGAAG
AAGATTGGCAACATTGGCAATTACTCACCCAAAAAGTTGGTTCACGGGTGCAGTTGGTTGGTGATGACTTGTTTGTAACA
AACGCTACTCGCTTACAAAAAGGCATCCAAGAAAAAGCAGGTAACGCCATTCTGATTAAACTCAATCAAATTGGTTCCCT
GACTGAAACCCTAGAAACCATCGACTTAGGAACTCGTAATGGTTTCCGTTCAGTCATTAGCCATCGTTCTGGTGAAACAG
AAGACACCACCATTGCTGATTTAGCCGTTGCTACCCGTGCAGGTCAAATCAAAACTGGTTCCCTCTGTCGTAGTGAACGG
GTAGCCAAATACAACCGCTTGCTACGTATTGAAGATGAATTAGGCGATCGCGCCGTTTATGCTGGGGCTGTGGGTTTAGG
GCCGAAATAG

Upstream 100 bases:

>100_bases
TTTAATCTCAGCCGATAAGTCGCCTCAGCGCCTATGCTAGCGATTGGGTTGAACCATTAGATTGTGTGCGACCAACGGTA
AGAAGGAGACGAATAGATCA

Downstream 100 bases:

>100_bases
GGACTGGGGATTAGGGACTGGGGACTGGGGACTGGGGACTGGGGACAAATTAAATCCTCTTTCATTACTCAATACTAAAT
ACCCAATACCCAATACCCGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNNIVDTAIEAIVAREILDSRGRPTIEAEVHLLSGAVGLAQVPSGASTGTFEAHELRDKDKSRYGGKGVLKAVHNVNEIL
APKLIDLDALNQELIDRTMIALDGSGNKSNLGANAILAVSLAAARAGAESLGIPLYRYLGGPLANLLPVPLMNVINGGAH
ASNNVDFQEFMIVPVGATSFREALRWGAEVFATLSEVLHDKGLLTGVGDEGGFAPNLESNQVALELLVAAIEKAGYKPGE
QVALALDVAASEFYKEGQYVYDGRPHAPTEFIDYLGQLVDQYPIVSIEDGLHEEDWQHWQLLTQKVGSRVQLVGDDLFVT
NATRLQKGIQEKAGNAILIKLNQIGSLTETLETIDLGTRNGFRSVISHRSGETEDTTIADLAVATRAGQIKTGSLCRSER
VAKYNRLLRIEDELGDRAVYAGAVGLGPK

Sequences:

>Translated_429_residues
MNNIVDTAIEAIVAREILDSRGRPTIEAEVHLLSGAVGLAQVPSGASTGTFEAHELRDKDKSRYGGKGVLKAVHNVNEIL
APKLIDLDALNQELIDRTMIALDGSGNKSNLGANAILAVSLAAARAGAESLGIPLYRYLGGPLANLLPVPLMNVINGGAH
ASNNVDFQEFMIVPVGATSFREALRWGAEVFATLSEVLHDKGLLTGVGDEGGFAPNLESNQVALELLVAAIEKAGYKPGE
QVALALDVAASEFYKEGQYVYDGRPHAPTEFIDYLGQLVDQYPIVSIEDGLHEEDWQHWQLLTQKVGSRVQLVGDDLFVT
NATRLQKGIQEKAGNAILIKLNQIGSLTETLETIDLGTRNGFRSVISHRSGETEDTTIADLAVATRAGQIKTGSLCRSER
VAKYNRLLRIEDELGDRAVYAGAVGLGPK
>Mature_429_residues
MNNIVDTAIEAIVAREILDSRGRPTIEAEVHLLSGAVGLAQVPSGASTGTFEAHELRDKDKSRYGGKGVLKAVHNVNEIL
APKLIDLDALNQELIDRTMIALDGSGNKSNLGANAILAVSLAAARAGAESLGIPLYRYLGGPLANLLPVPLMNVINGGAH
ASNNVDFQEFMIVPVGATSFREALRWGAEVFATLSEVLHDKGLLTGVGDEGGFAPNLESNQVALELLVAAIEKAGYKPGE
QVALALDVAASEFYKEGQYVYDGRPHAPTEFIDYLGQLVDQYPIVSIEDGLHEEDWQHWQLLTQKVGSRVQLVGDDLFVT
NATRLQKGIQEKAGNAILIKLNQIGSLTETLETIDLGTRNGFRSVISHRSGETEDTTIADLAVATRAGQIKTGSLCRSER
VAKYNRLLRIEDELGDRAVYAGAVGLGPK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=54.7785547785548, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI301897477, Length=436, Percent_Identity=52.9816513761468, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=436, Percent_Identity=52.9816513761468, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=52.9137529137529, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI301897479, Length=434, Percent_Identity=47.926267281106, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=26.5671641791045, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=26.5671641791045, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=26.5671641791045, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1789141, Length=423, Percent_Identity=58.628841607565, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=53.3799533799534, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=53.3799533799534, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=48.9473684210526, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.4226327944573, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.1916859122402, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.1916859122402, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=47.9166666666667, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=47.6851851851852, Blast_Score=344, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24580918, Length=441, Percent_Identity=50.3401360544218, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580916, Length=441, Percent_Identity=50.3401360544218, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580920, Length=441, Percent_Identity=50.3401360544218, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580914, Length=441, Percent_Identity=50.3401360544218, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281360527, Length=438, Percent_Identity=50.6849315068493, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=438, Percent_Identity=50.6849315068493, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45966; Mature: 45966

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIVDTAIEAIVAREILDSRGRPTIEAEVHLLSGAVGLAQVPSGASTGTFEAHELRDKD
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEHHCCCCHHCCCCCCCCCCCCHHHHHHHH
KSRYGGKGVLKAVHNVNEILAPKLIDLDALNQELIDRTMIALDGSGNKSNLGANAILAVS
HHCCCCHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHH
LAAARAGAESLGIPLYRYLGGPLANLLPVPLMNVINGGAHASNNVDFQEFMIVPVGATSF
HHHHHCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECCHHHH
REALRWGAEVFATLSEVLHDKGLLTGVGDEGGFAPNLESNQVALELLVAAIEKAGYKPGE
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCH
QVALALDVAASEFYKEGQYVYDGRPHAPTEFIDYLGQLVDQYPIVSIEDGLHEEDWQHWQ
HEEEEHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHH
LLTQKVGSRVQLVGDDLFVTNATRLQKGIQEKAGNAILIKLNQIGSLTETLETIDLGTRN
HHHHHHCCEEEEECCCEEEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCH
GFRSVISHRSGETEDTTIADLAVATRAGQIKTGSLCRSERVAKYNRLLRIEDELGDRAVY
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEHHHCCCCEEE
AGAVGLGPK
ECCCCCCCC
>Mature Secondary Structure
MNNIVDTAIEAIVAREILDSRGRPTIEAEVHLLSGAVGLAQVPSGASTGTFEAHELRDKD
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEHHCCCCHHCCCCCCCCCCCCHHHHHHHH
KSRYGGKGVLKAVHNVNEILAPKLIDLDALNQELIDRTMIALDGSGNKSNLGANAILAVS
HHCCCCHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHH
LAAARAGAESLGIPLYRYLGGPLANLLPVPLMNVINGGAHASNNVDFQEFMIVPVGATSF
HHHHHCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECCHHHH
REALRWGAEVFATLSEVLHDKGLLTGVGDEGGFAPNLESNQVALELLVAAIEKAGYKPGE
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCH
QVALALDVAASEFYKEGQYVYDGRPHAPTEFIDYLGQLVDQYPIVSIEDGLHEEDWQHWQ
HEEEEHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHH
LLTQKVGSRVQLVGDDLFVTNATRLQKGIQEKAGNAILIKLNQIGSLTETLETIDLGTRN
HHHHHHCCEEEEECCCEEEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCH
GFRSVISHRSGETEDTTIADLAVATRAGQIKTGSLCRSERVAKYNRLLRIEDELGDRAVY
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEHHHCCCCEEE
AGAVGLGPK
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA