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The map label for this gene is gca [H]

Identifier: 17230916

GI number: 17230916

Start: 4132791

End: 4133795

Strand: Direct

Name: gca [H]

Synonym: alr3424

Alternate gene names: 17230916

Gene position: 4132791-4133795 (Clockwise)

Preceding gene: 17230915

Following gene: 17230917

Centisome position: 64.44

GC content: 42.39

Gene sequence:

>1005_bases
ATGACTAAAACAGCCTTAGTTACAGGTATTACTGGTCAAGATGGCTACTATCTCAGCCATTTGCTCCTCAATCAAGGTTA
TCGAGTAGTGGGATTAGTTTCACCACACCGCCAACCGAATTTGGCAAAGTTAGGGGATTTGGCAAACAAGGTAGAAATAT
ATAATGTTGATTTGAGAGATCCTGTAGCATTGTTAAATGCAGTGGAACAACTGCGTCCCCAAGAAATTTATAATTTAGCT
GCACCTAGTTTTGTGCCTGATTCGTGGAAAGACCCACTAGGAACTTTGGATCTGATTACTGGGACTGCTACCCGACTGTT
AGATGCAGTCCGACAGGTTGGTTTATCTACAAGATTCTATCAAGCTAGTAGTTCCGAGATGTTTGGGGATGTTTTATCTT
CTCCTCAAGATGAAGAAACCAGCTTTCGTCCCAAAAATCCCTACGCAGCAGCAAAGTTACACGCCCATTGGACAATGGTG
CATCACCGACAGCGCTATGGTCTATTTGCCTGTAGTGGCATTTTATATAACCATGAGTCACCTTTACGCCCGCCTCAATT
TGTTACGCGTAAAGTTTCTTTGGCTGCTGCATCGATTAAGTTGGGTTTAGCTCAGACACTGGAAATGGGTAACTTAGATG
CTAAACGTGATTGGGGATTTGCTGGCGATTACGTCAAAGCTATGTGGCTGATGTTGCAAGCAGAGGAACCAGAAGAGTAC
GTAATAGGTACAGGAAAACTGCACAGTGTTAAAGAATTAGTGTCTATCGCTTTTGAGTCGGTGGGCTTAAACTGGCAAAA
TCATGTAGTGATTAATACAGATTTGCTCAGACAAGACGAACATTTTCAGCTCGTAGCTAACCCTATTAAAGCTAAAAAGA
ACCTTGGTTGGGAAACGGAAGTTAGCTTTGAGAGACTTTTAGAGAAAATGGTGCAAACAGATGTAGAAAGATTACAAAGC
GGTGCGATCGCCCAAGCATCAGAAGGAAAATTACTGGTAGATTAG

Upstream 100 bases:

>100_bases
AAGTCGAATTGAGATGTTTATAAGTAGAATAAACCCTCAGTAATTCTTCCCTAGCCTGCTGTAGCTCTTTATTGGTCAAA
GAGGGGATGGGGAAGAGGAT

Downstream 100 bases:

>100_bases
GATACCGTAGCGCACTTGCACCCATCACCGTACTATCTCTAAAAATCAATAGAAAGAATCTCAATGCCAATTGATACAGT
GAGAGAACAAATAAGTTTAG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTKTALVTGITGQDGYYLSHLLLNQGYRVVGLVSPHRQPNLAKLGDLANKVEIYNVDLRDPVALLNAVEQLRPQEIYNLA
APSFVPDSWKDPLGTLDLITGTATRLLDAVRQVGLSTRFYQASSSEMFGDVLSSPQDEETSFRPKNPYAAAKLHAHWTMV
HHRQRYGLFACSGILYNHESPLRPPQFVTRKVSLAAASIKLGLAQTLEMGNLDAKRDWGFAGDYVKAMWLMLQAEEPEEY
VIGTGKLHSVKELVSIAFESVGLNWQNHVVINTDLLRQDEHFQLVANPIKAKKNLGWETEVSFERLLEKMVQTDVERLQS
GAIAQASEGKLLVD

Sequences:

>Translated_334_residues
MTKTALVTGITGQDGYYLSHLLLNQGYRVVGLVSPHRQPNLAKLGDLANKVEIYNVDLRDPVALLNAVEQLRPQEIYNLA
APSFVPDSWKDPLGTLDLITGTATRLLDAVRQVGLSTRFYQASSSEMFGDVLSSPQDEETSFRPKNPYAAAKLHAHWTMV
HHRQRYGLFACSGILYNHESPLRPPQFVTRKVSLAAASIKLGLAQTLEMGNLDAKRDWGFAGDYVKAMWLMLQAEEPEEY
VIGTGKLHSVKELVSIAFESVGLNWQNHVVINTDLLRQDEHFQLVANPIKAKKNLGWETEVSFERLLEKMVQTDVERLQS
GAIAQASEGKLLVD
>Mature_333_residues
TKTALVTGITGQDGYYLSHLLLNQGYRVVGLVSPHRQPNLAKLGDLANKVEIYNVDLRDPVALLNAVEQLRPQEIYNLAA
PSFVPDSWKDPLGTLDLITGTATRLLDAVRQVGLSTRFYQASSSEMFGDVLSSPQDEETSFRPKNPYAAAKLHAHWTMVH
HRQRYGLFACSGILYNHESPLRPPQFVTRKVSLAAASIKLGLAQTLEMGNLDAKRDWGFAGDYVKAMWLMLQAEEPEEYV
IGTGKLHSVKELVSIAFESVGLNWQNHVVINTDLLRQDEHFQLVANPIKAKKNLGWETEVSFERLLEKMVQTDVERLQSG
AIAQASEGKLLVD

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=42.8571428571429, Blast_Score=274, Evalue=9e-74,
Organism=Escherichia coli, GI1788366, Length=352, Percent_Identity=40.0568181818182, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1788353, Length=368, Percent_Identity=24.7282608695652, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994969, Length=333, Percent_Identity=25.2252252252252, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=349, Percent_Identity=39.8280802292264, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI133901788, Length=349, Percent_Identity=39.8280802292264, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI133901790, Length=349, Percent_Identity=39.8280802292264, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17539422, Length=349, Percent_Identity=39.8280802292264, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI133901786, Length=349, Percent_Identity=39.8280802292264, Blast_Score=264, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17507723, Length=349, Percent_Identity=39.541547277937, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24158427, Length=349, Percent_Identity=38.9684813753582, Blast_Score=264, Evalue=5e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 37266; Mature: 37135

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTALVTGITGQDGYYLSHLLLNQGYRVVGLVSPHRQPNLAKLGDLANKVEIYNVDLRD
CCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCEEEEEEECCCC
PVALLNAVEQLRPQEIYNLAAPSFVPDSWKDPLGTLDLITGTATRLLDAVRQVGLSTRFY
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
QASSSEMFGDVLSSPQDEETSFRPKNPYAAAKLHAHWTMVHHRQRYGLFACSGILYNHES
CCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCC
PLRPPQFVTRKVSLAAASIKLGLAQTLEMGNLDAKRDWGFAGDYVKAMWLMLQAEEPEEY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
VIGTGKLHSVKELVSIAFESVGLNWQNHVVINTDLLRQDEHFQLVANPIKAKKNLGWETE
EEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHCCCCCCC
VSFERLLEKMVQTDVERLQSGAIAQASEGKLLVD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECC
>Mature Secondary Structure 
TKTALVTGITGQDGYYLSHLLLNQGYRVVGLVSPHRQPNLAKLGDLANKVEIYNVDLRD
CCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCEEEEEEECCCC
PVALLNAVEQLRPQEIYNLAAPSFVPDSWKDPLGTLDLITGTATRLLDAVRQVGLSTRFY
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
QASSSEMFGDVLSSPQDEETSFRPKNPYAAAKLHAHWTMVHHRQRYGLFACSGILYNHES
CCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCC
PLRPPQFVTRKVSLAAASIKLGLAQTLEMGNLDAKRDWGFAGDYVKAMWLMLQAEEPEEY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
VIGTGKLHSVKELVSIAFESVGLNWQNHVVINTDLLRQDEHFQLVANPIKAKKNLGWETE
EEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHCCCCCCC
VSFERLLEKMVQTDVERLQSGAIAQASEGKLLVD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]