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The map label for this gene is spo0F [H]

Identifier: 17230878

GI number: 17230878

Start: 4097421

End: 4097804

Strand: Direct

Name: spo0F [H]

Synonym: alr3386

Alternate gene names: 17230878

Gene position: 4097421-4097804 (Clockwise)

Preceding gene: 17230877

Following gene: 17230879

Centisome position: 63.88

GC content: 38.8

Gene sequence:

>384_bases
ATGAACACTCTGAATTTAGGAAGAGGAAAGGTTTTAATAGCTGATGATGATGAAGATAGCCGAATGATGCTCTCTTTTGT
ATTACAGGAAGAGGGGTGGGAAGTCTATGAGGCTTGTAACGGCAAAGAAGCTCTAGAAAAAGTCATTGAAGAGAAACCGG
ATTTATTGATTTTAGATAATAGAATGCCAGAGTTAAGCGGCGTTGAAGTTTATCAATATCTACAAGCAGAAGGTATTAAC
ATAGCGGTTGTCTTAGCCACAGCATACGGTTATTTGGACGAATTAGCTTCATCCTTGGGGGTGGAGTATTTTATTCATAA
ACCCTATGAGATTCCCAAGTTACTGAAAATGGTAGAGTCTGCTTACGCTCATTCTCGTAACTAA

Upstream 100 bases:

>100_bases
ACTTGTCCTAGTGAGTTTTCTGGCTATTGCAGCCTTAAATACATATCCATTAGAGCCAACCCCATTAGTTTTTTCCATAC
CAATAAAAAATTAATAATTT

Downstream 100 bases:

>100_bases
CTTAGTTACTTTCAGGCTGGTGGGAGATTGTCGGGGTCAACACCGAGACTACGCAAATAAGCTGCTAACTGTTCTACACG
TTGGCGTTTTTGTTCTAACA

Product: two-component response regulator

Products: NA

Alternate protein names: Stage 0 sporulation protein F [H]

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MNTLNLGRGKVLIADDDEDSRMMLSFVLQEEGWEVYEACNGKEALEKVIEEKPDLLILDNRMPELSGVEVYQYLQAEGIN
IAVVLATAYGYLDELASSLGVEYFIHKPYEIPKLLKMVESAYAHSRN

Sequences:

>Translated_127_residues
MNTLNLGRGKVLIADDDEDSRMMLSFVLQEEGWEVYEACNGKEALEKVIEEKPDLLILDNRMPELSGVEVYQYLQAEGIN
IAVVLATAYGYLDELASSLGVEYFIHKPYEIPKLLKMVESAYAHSRN
>Mature_127_residues
MNTLNLGRGKVLIADDDEDSRMMLSFVLQEEGWEVYEACNGKEALEKVIEEKPDLLILDNRMPELSGVEVYQYLQAEGIN
IAVVLATAYGYLDELASSLGVEYFIHKPYEIPKLLKMVESAYAHSRN

Specific function: Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=30.7017543859649, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1790299, Length=121, Percent_Identity=30.5785123966942, Blast_Score=62, Evalue=8e-12,
Organism=Escherichia coli, GI1790437, Length=120, Percent_Identity=31.6666666666667, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 14375; Mature: 14375

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLNLGRGKVLIADDDEDSRMMLSFVLQEEGWEVYEACNGKEALEKVIEEKPDLLILDN
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCEEEECC
RMPELSGVEVYQYLQAEGINIAVVLATAYGYLDELASSLGVEYFIHKPYEIPKLLKMVES
CCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
AYAHSRN
HHHCCCC
>Mature Secondary Structure
MNTLNLGRGKVLIADDDEDSRMMLSFVLQEEGWEVYEACNGKEALEKVIEEKPDLLILDN
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCEEEECC
RMPELSGVEVYQYLQAEGINIAVVLATAYGYLDELASSLGVEYFIHKPYEIPKLLKMVES
CCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
AYAHSRN
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7514590 [H]