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The map label for this gene is spsK [H]

Identifier: 17230828

GI number: 17230828

Start: 4032027

End: 4032911

Strand: Direct

Name: spsK [H]

Synonym: alr3336

Alternate gene names: 17230828

Gene position: 4032027-4032911 (Clockwise)

Preceding gene: 17230823

Following gene: 17230829

Centisome position: 62.87

GC content: 37.85

Gene sequence:

>885_bases
ATGCAGAAGCTTTTAATTACTGGTGCTAGCGGTTTTTTAGGATGGCACCTTTGCCAAGTAGCCCAACAAGAATGGGAAGT
TTATGGAACTTATTGCTCTCATGCTTTATCTATTCAAGGTATAAAATTACTCAAGGTCAATTTAACAGATTTTTCAGAAA
TCAAACAGATATTTAATGAAATTAAACCAGATGCAGTCATACATACAGCCGCACAATCACAACCAAATTTTTGTCAAATC
CATCCTGATGAATCATATAAAATTAACGTCACTGCCTCCTGCAATCTTGCCGAACTTTGTGCAACATCTTCTATCCCTTA
TGCTTTTACATCCAGCGAACTAGTTTTTGATGGCGTAAATTCTCCTTATAAAGAAACTGACACAGTATGTCCGGTGAATA
TTTATGGTGAGCAGAAAGTCTTGGCTGAAGTGGGTATATTAGAACGTTATCCCATAGCAACAATATGTCGAATGCCTTTG
ATGTTTGGTAATGCTACACCTACAGCTAAAAGCTTTATTCAGCCATTTATTCAAACTTTACAATCTGGTCAAGAATTGAC
TTTATTTATAGATGAATTTCGTACCCCTGTTAGTGGCACAACGGCAGCTAAAGGAATTTTATTAACATTAGGAAAAGTTA
CAGGACTAATTCATTTAGGTGGCAAAGAACGGATTTCCCGTTATGATTTCGGGCATATATTAGCCGCAGTCTTTCAATTA
CCAACCACAGGAATTAAAGCCTGTCTTCAACGAGATGTAAACATGGCAGCACCCAGGCCAGCAGATGTTTCCTTAGATAG
TTCACAGGCTTTTGCTTTAGGTTATCAACCTTTATCTATCAAGTCAGAATTAGAGGCATTGCTTAACAAGGCAGGGGGCG
ATTAG

Upstream 100 bases:

>100_bases
CAGGAAATAATGATGCAGTAATTAAAAATATTCAAGTTTTTGAAACTATTAAGAGTATAATTTTTGCGTCCATCATCTCA
ATATACGTACTAGTTTCGAT

Downstream 100 bases:

>100_bases
TGAGAGATGCAACTGCTCTTTGATTCAGAATGTGAGGATGGGCGATCGCTTTTACACCCATTCATTTTTCTCAAAAATAT
TAAGCAATCCTTAAAATTAT

Product: hypothetical protein

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MQKLLITGASGFLGWHLCQVAQQEWEVYGTYCSHALSIQGIKLLKVNLTDFSEIKQIFNEIKPDAVIHTAAQSQPNFCQI
HPDESYKINVTASCNLAELCATSSIPYAFTSSELVFDGVNSPYKETDTVCPVNIYGEQKVLAEVGILERYPIATICRMPL
MFGNATPTAKSFIQPFIQTLQSGQELTLFIDEFRTPVSGTTAAKGILLTLGKVTGLIHLGGKERISRYDFGHILAAVFQL
PTTGIKACLQRDVNMAAPRPADVSLDSSQAFALGYQPLSIKSELEALLNKAGGD

Sequences:

>Translated_294_residues
MQKLLITGASGFLGWHLCQVAQQEWEVYGTYCSHALSIQGIKLLKVNLTDFSEIKQIFNEIKPDAVIHTAAQSQPNFCQI
HPDESYKINVTASCNLAELCATSSIPYAFTSSELVFDGVNSPYKETDTVCPVNIYGEQKVLAEVGILERYPIATICRMPL
MFGNATPTAKSFIQPFIQTLQSGQELTLFIDEFRTPVSGTTAAKGILLTLGKVTGLIHLGGKERISRYDFGHILAAVFQL
PTTGIKACLQRDVNMAAPRPADVSLDSSQAFALGYQPLSIKSELEALLNKAGGD
>Mature_294_residues
MQKLLITGASGFLGWHLCQVAQQEWEVYGTYCSHALSIQGIKLLKVNLTDFSEIKQIFNEIKPDAVIHTAAQSQPNFCQI
HPDESYKINVTASCNLAELCATSSIPYAFTSSELVFDGVNSPYKETDTVCPVNIYGEQKVLAEVGILERYPIATICRMPL
MFGNATPTAKSFIQPFIQTLQSGQELTLFIDEFRTPVSGTTAAKGILLTLGKVTGLIHLGGKERISRYDFGHILAAVFQL
PTTGIKACLQRDVNMAAPRPADVSLDSSQAFALGYQPLSIKSELEALLNKAGGD

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=283, Percent_Identity=28.9752650176678, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI11034825, Length=283, Percent_Identity=28.9752650176678, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1788352, Length=248, Percent_Identity=23.3870967741935, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 32039; Mature: 32039

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLLITGASGFLGWHLCQVAQQEWEVYGTYCSHALSIQGIKLLKVNLTDFSEIKQIFNE
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCHHHHHHHHHH
IKPDAVIHTAAQSQPNFCQIHPDESYKINVTASCNLAELCATSSIPYAFTSSELVFDGVN
CCCCCEEEEHHCCCCCEEEECCCCCEEEEEEECCCHHHHHHCCCCCEEECCCCEEEECCC
SPYKETDTVCPVNIYGEQKVLAEVGILERYPIATICRMPLMFGNATPTAKSFIQPFIQTL
CCCCCCCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QSGQELTLFIDEFRTPVSGTTAAKGILLTLGKVTGLIHLGGKERISRYDFGHILAAVFQL
HCCCEEEEEEEHHCCCCCCCHHHHHHHEEHHHHHEEEEECCHHHHHHCCHHHHHHHHHHC
PTTGIKACLQRDVNMAAPRPADVSLDSSQAFALGYQPLSIKSELEALLNKAGGD
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKLLITGASGFLGWHLCQVAQQEWEVYGTYCSHALSIQGIKLLKVNLTDFSEIKQIFNE
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCHHHHHHHHHH
IKPDAVIHTAAQSQPNFCQIHPDESYKINVTASCNLAELCATSSIPYAFTSSELVFDGVN
CCCCCEEEEHHCCCCCEEEECCCCCEEEEEEECCCHHHHHHCCCCCEEECCCCEEEECCC
SPYKETDTVCPVNIYGEQKVLAEVGILERYPIATICRMPLMFGNATPTAKSFIQPFIQTL
CCCCCCCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QSGQELTLFIDEFRTPVSGTTAAKGILLTLGKVTGLIHLGGKERISRYDFGHILAAVFQL
HCCCEEEEEEEHHCCCCCCCHHHHHHHEEHHHHHEEEEECCHHHHHHCCHHHHHHHHHHC
PTTGIKACLQRDVNMAAPRPADVSLDSSQAFALGYQPLSIKSELEALLNKAGGD
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]