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The map label for this gene is mazF [H]

Identifier: 17230703

GI number: 17230703

Start: 3880315

End: 3880755

Strand: Reverse

Name: mazF [H]

Synonym: all3211

Alternate gene names: 17230703

Gene position: 3880755-3880315 (Counterclockwise)

Preceding gene: 17230704

Following gene: 17230701

Centisome position: 60.51

GC content: 36.51

Gene sequence:

>441_bases
GTGAAGCCGCCTTACTTTCCCAATAGAGGAGACATTGTTAAATTAGAGTTTGGCTCTGCACAACAGTTCACGGCTGAATC
AATTCAGCGTGTATTTACCCTTCGTAATTCTGGAATGTCATTTGATGATATTGCCATAACACTAAATAACGAGCTACAAC
AACAAGGGCGTGAGCAAACTGGCTATCGCCCTGTTCTTGTTATATCTCCAATTAAGTACAATCAAATGGCTTCTTTAGTT
TTAGCTTGTCCTATAACTACTAACGCAAAGGGGCTTAGGTTTGAAGTTCCCCTTATTGAAGGAATGAAAACAAAAGGGGT
TGTGTTAGCAGATCAAATTAAAACACTAGATTGGAAAGCTAGAAAAGTAAAATTTGTTGAAAGTGTAACAGAAGATTTAA
TAGAAGAAGTACAAGCAAAACTCGAAACATTAATTTTATAG

Upstream 100 bases:

>100_bases
GAAAAAAATATACGCTTGATGAGTTACTTGAAGGGATGACCCCTGATAATTTTCATCCAGAATTTGAAACAGGTGACGCT
GTGGGGAATGAAGCTTGGTA

Downstream 100 bases:

>100_bases
AAAAAACAATTGTGTTAATCCAAATAGCAAGAAAGCATTCTACTGTGTTTTTTACTGTCTACTTTCTTGACCAATAACTA
ATGACTAGACTGAAAACCTA

Product: cell growth regulatory protein

Products: NA

Alternate protein names: Endoribonuclease MazF; Toxin MazF [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKPPYFPNRGDIVKLEFGSAQQFTAESIQRVFTLRNSGMSFDDIAITLNNELQQQGREQTGYRPVLVISPIKYNQMASLV
LACPITTNAKGLRFEVPLIEGMKTKGVVLADQIKTLDWKARKVKFVESVTEDLIEEVQAKLETLIL

Sequences:

>Translated_146_residues
MKPPYFPNRGDIVKLEFGSAQQFTAESIQRVFTLRNSGMSFDDIAITLNNELQQQGREQTGYRPVLVISPIKYNQMASLV
LACPITTNAKGLRFEVPLIEGMKTKGVVLADQIKTLDWKARKVKFVESVTEDLIEEVQAKLETLIL
>Mature_146_residues
MKPPYFPNRGDIVKLEFGSAQQFTAESIQRVFTLRNSGMSFDDIAITLNNELQQQGREQTGYRPVLVISPIKYNQMASLV
LACPITTNAKGLRFEVPLIEGMKTKGVVLADQIKTLDWKARKVKFVESVTEDLIEEVQAKLETLIL

Specific function: Part of a toxin-antitoxin (TA) module. MazF is a sequence-specific mRNA endoribonuclease that inhibits protein synthesis and induces bacterial stasis. It is very stable, single- strand specific and cleavage is independent of the ribosome. The endoribonucl

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

Organism=Escherichia coli, GI1789145, Length=91, Percent_Identity=39.5604395604396, Blast_Score=77, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 16460; Mature: 16460

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPYFPNRGDIVKLEFGSAQQFTAESIQRVFTLRNSGMSFDDIAITLNNELQQQGREQT
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHCCHHHC
GYRPVLVISPIKYNQMASLVLACPITTNAKGLRFEVPLIEGMKTKGVVLADQIKTLDWKA
CCCCEEEECCCCHHHHHHEEEECCCCCCCCCEEEECCHHCCCCCCCEEEECCHHCCCCHH
RKVKFVESVTEDLIEEVQAKLETLIL
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPPYFPNRGDIVKLEFGSAQQFTAESIQRVFTLRNSGMSFDDIAITLNNELQQQGREQT
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHCCHHHC
GYRPVLVISPIKYNQMASLVLACPITTNAKGLRFEVPLIEGMKTKGVVLADQIKTLDWKA
CCCCEEEECCCCHHHHHHEEEECCCCCCCCCEEEECCHHCCCCCCCEEEECCHHCCCCHH
RKVKFVESVTEDLIEEVQAKLETLIL
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]