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The map label for this gene is ppsA [H]

Identifier: 17230639

GI number: 17230639

Start: 3810379

End: 3810870

Strand: Direct

Name: ppsA [H]

Synonym: alr3147

Alternate gene names: 17230639

Gene position: 3810379-3810870 (Clockwise)

Preceding gene: 17230638

Following gene: 17230642

Centisome position: 59.41

GC content: 45.53

Gene sequence:

>492_bases
GTGCGGGATGAAATGGGGTTAACTAACGTTATTCCCATGATTCCTTTCTGTCGCACACCAGAGGAGGGACGTTTGGTTTT
AGCAGAGATGGCGAAACATGGTTTAAAGCAAGGTGTTAACGACTTGCAGGTTTATGTAATGTGCGAGTTACCAAGTAACG
TCATCTTGGCCGAACAGTTTGCAGAGGTGTTCGACGGTTTCTCCATTGGTTCCAACGACCTGACTCAGCTGACACTAGGA
TTAGATAGAGATTCAGCTTTAGTCGCTCGACTATTTGACGAACGTAGCCCAGCTGTGAAACAAATGGTGAAAATGGCGAT
CGCCTCTGCAAAAAAACAACGCCGCAAAATCGGCATCTGTGGGCAAGCACCAAGCGATTACCCGGAATTTGCCCAGTTTT
TGGTAGAAGAAGGAATTGATTCTATTAGTCTCAATCCCGACTCTGTATTAAAAACGATGCTGGAAATAGCTAAGGTGGAA
CAGCAAAGCTGA

Upstream 100 bases:

>100_bases
GATGAAGAAAACCCGATGCTGGGCTGGCGGGGGGGCTGCACGTTATTACGATCCAGGTTATAGAGAAGGTTTTGCTTTAG
AATGCCATGCTATCAAGCGG

Downstream 100 bases:

>100_bases
GGTAGGAGGTAAATCAATTCAAAATACCCTACGGGTTCGCGTAGCGTTCTCGAAGAGTAGGCAAGCCTACAAAATTATAG
AATTGCTGACTCAGCACTCG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MRDEMGLTNVIPMIPFCRTPEEGRLVLAEMAKHGLKQGVNDLQVYVMCELPSNVILAEQFAEVFDGFSIGSNDLTQLTLG
LDRDSALVARLFDERSPAVKQMVKMAIASAKKQRRKIGICGQAPSDYPEFAQFLVEEGIDSISLNPDSVLKTMLEIAKVE
QQS

Sequences:

>Translated_163_residues
MRDEMGLTNVIPMIPFCRTPEEGRLVLAEMAKHGLKQGVNDLQVYVMCELPSNVILAEQFAEVFDGFSIGSNDLTQLTLG
LDRDSALVARLFDERSPAVKQMVKMAIASAKKQRRKIGICGQAPSDYPEFAQFLVEEGIDSISLNPDSVLKTMLEIAKVE
QQS
>Mature_163_residues
MRDEMGLTNVIPMIPFCRTPEEGRLVLAEMAKHGLKQGVNDLQVYVMCELPSNVILAEQFAEVFDGFSIGSNDLTQLTLG
LDRDSALVARLFDERSPAVKQMVKMAIASAKKQRRKIGICGQAPSDYPEFAQFLVEEGIDSISLNPDSVLKTMLEIAKVE
QQS

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=161, Percent_Identity=59.6273291925466, Blast_Score=211, Evalue=2e-56,
Organism=Escherichia coli, GI1788756, Length=145, Percent_Identity=30.3448275862069, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI48994992, Length=118, Percent_Identity=35.5932203389831, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788726, Length=96, Percent_Identity=30.2083333333333, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1789193, Length=118, Percent_Identity=28.8135593220339, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 18034; Mature: 18034

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDEMGLTNVIPMIPFCRTPEEGRLVLAEMAKHGLKQGVNDLQVYVMCELPSNVILAEQF
CCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHH
AEVFDGFSIGSNDLTQLTLGLDRDSALVARLFDERSPAVKQMVKMAIASAKKQRRKIGIC
HHHHCCCCCCCCCHHHEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
GQAPSDYPEFAQFLVEEGIDSISLNPDSVLKTMLEIAKVEQQS
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRDEMGLTNVIPMIPFCRTPEEGRLVLAEMAKHGLKQGVNDLQVYVMCELPSNVILAEQF
CCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHH
AEVFDGFSIGSNDLTQLTLGLDRDSALVARLFDERSPAVKQMVKMAIASAKKQRRKIGIC
HHHHCCCCCCCCCHHHEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
GQAPSDYPEFAQFLVEEGIDSISLNPDSVLKTMLEIAKVEQQS
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]