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The map label for this gene is ppsA [H]

Identifier: 17230638

GI number: 17230638

Start: 3808410

End: 3810341

Strand: Direct

Name: ppsA [H]

Synonym: alr3146

Alternate gene names: 17230638

Gene position: 3808410-3810341 (Clockwise)

Preceding gene: 17230634

Following gene: 17230639

Centisome position: 59.38

GC content: 44.51

Gene sequence:

>1932_bases
ATGGTGACAGTATCTCAAAACACTTTGGCAAAATTTTCTCAAGAGGAATCGTTGGTTCTCTGGCTTGATAAAGTTGGCAT
TAAAGATATTGCCTTAGTGGGTGGTAAAAATGCCTCATTGGGGGAAATGATTCAACAACTGACACCCAAAGGCGTGAACG
TACCGACAGGATTTGCTACTACTGCTTACGCTTATCGTTATTTCATCCAATCGGCTGGGTTAGAAGCCAAGTTACGGGAA
CTGTTTGCAGATTTAGATGTGGAGGATGTGAGAAATTTACAAGAACGGGGACTCAAAGCCCGATCGCTCCTCATGCACAC
CCCATTTCCTTTAGAATTACAACAGGCGATCGCCTCTGCTTATGAAAGTTTGTGTAAGCAGTACAATCCTGATACAGATG
TCGCCGTCCGTTCCAGCGCTACCGCCGAAGACTTACCTGATGCGAGTTTTGCTGGACAACAAGAAACATATCTCAACGTA
GTTGGTGCAGAAGCAGTATTAGCAGCTTGTCATAAATGCTTTGCTTCCCTATTTACAGACCGCGCCATTTCCTATCGCCA
TACTAAAGGATTTGATCACTTTAGCGTCGCCTTGGCTGTGGGTGTACAGAAAATGGTACGTTCTGACCTAGCCACCTCTG
GGGTGATGTTCTCTATTGATACAGAAACAGGTTTTAAAGATGCAGCCTTAATTACAGCCGCCTATGGTTTAGGCGAAAAC
GTTGTCCAAGGGACAGTTAATCCAGATGAATATTGTGTATTTAAGCCGACATTAAAAACAGGATTTAAACCAATTATTGA
TAAAAAATTGGGCAGTAAAGAATTAAGAATGGTCTATGATGAAGGCTCCCAATCTACTAAAAATGAACCAGTATCAATAG
TAGAACAAAATAAATTTGCTCTCAGTGACGCAGATATTTTACAACTAGCTCATTGGGCTTGTTTAATTGAAGACCATTAT
TCTCAAGCCCACGGTAGTTACACCCCAATGGATATTGAATGGGCAAAAGATGGCATTACCAACCAACTTTTTGTAGTGCA
AGCCCGTCCAGAAACGGTGCAGTCCCAGAAGAATGGGAATGTGTTGCGGAGTTATCGGTTGGGGCTGGGGACTGGGGAGA
AAACGCAGAATTTATCACTCATCCCCCTAGTCACTGGACGCGCCATTGGTGAAGCGATTAGCCAAGGGAAAGCGCGGGTA
ATTTTAGATGCCAAACAAATAGAAGAATTTCAGGCTGGGGAGGTGCTGGTGACAGAGAGAACTGACCCTGATTGGGAACC
AATTATGAAACGTGCCAGTGCAATTATTACCAATTCTGGTGGTCGTACTTGTCATGCGGCCATTATCGCACGGGAATTGG
GTGTGCCGGCGATCGTTGGTTGTGGTAAAGCCACGCAAGTATTAACAACTGGTCAAGAGGTGACGGTTTCTTGTGCGGAG
GGTGAAGAAGGTAAAGTCTATCCTGGGTTATTACCTTTTCAAGTGAAAGAAGTTCTCTTAGAAAACTTACCCCGCACCCA
TACCCAAATCTTGATGAATGTGGGTAATCCCCAAGAAGCGTTTAGTTTATCGGCAATTCCTAATGATGGTGTTGGTTTAG
CCAGGACGGAGTTTATCATTGCTAACCAAATCCAAGTCCATCCCTTGGCGTTGATTCACTACGACAATTTAGCGGATGCA
GAACAAAAAGCCAAAGTTGCGGCTATCACCGCCATGTATGACGATAAACCCCAATATTTTGTAGATAAATTAGCCCAAGG
TGTGGGCAGAATTGCCGCCGCCTTTTATCCCAAACCTGTGATTGTGCGAATGTCCGATTTTAAGAGTAATGAATATCGCA
ACCTTGCAGGGGGTTCCCAATTTGAACCAGATGAAGAAAACCCGATGCTGGGCTGGCGGGGGGGCTGCACGTTATTACGA
TCCAGGTTATAG

Upstream 100 bases:

>100_bases
GCTGCGATGGCGTTCTGTCTGACCGCCAAAAGAAAAAAAATAAATGCAACAAAAAACTAATTCTCCATAACCAAAGCATT
AATTCAGGGAGGTAATACTG

Downstream 100 bases:

>100_bases
AGAAGGTTTTGCTTTAGAATGCCATGCTATCAAGCGGGTGCGGGATGAAATGGGGTTAACTAACGTTATTCCCATGATTC
CTTTCTGTCGCACACCAGAG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MVTVSQNTLAKFSQEESLVLWLDKVGIKDIALVGGKNASLGEMIQQLTPKGVNVPTGFATTAYAYRYFIQSAGLEAKLRE
LFADLDVEDVRNLQERGLKARSLLMHTPFPLELQQAIASAYESLCKQYNPDTDVAVRSSATAEDLPDASFAGQQETYLNV
VGAEAVLAACHKCFASLFTDRAISYRHTKGFDHFSVALAVGVQKMVRSDLATSGVMFSIDTETGFKDAALITAAYGLGEN
VVQGTVNPDEYCVFKPTLKTGFKPIIDKKLGSKELRMVYDEGSQSTKNEPVSIVEQNKFALSDADILQLAHWACLIEDHY
SQAHGSYTPMDIEWAKDGITNQLFVVQARPETVQSQKNGNVLRSYRLGLGTGEKTQNLSLIPLVTGRAIGEAISQGKARV
ILDAKQIEEFQAGEVLVTERTDPDWEPIMKRASAIITNSGGRTCHAAIIARELGVPAIVGCGKATQVLTTGQEVTVSCAE
GEEGKVYPGLLPFQVKEVLLENLPRTHTQILMNVGNPQEAFSLSAIPNDGVGLARTEFIIANQIQVHPLALIHYDNLADA
EQKAKVAAITAMYDDKPQYFVDKLAQGVGRIAAAFYPKPVIVRMSDFKSNEYRNLAGGSQFEPDEENPMLGWRGGCTLLR
SRL

Sequences:

>Translated_643_residues
MVTVSQNTLAKFSQEESLVLWLDKVGIKDIALVGGKNASLGEMIQQLTPKGVNVPTGFATTAYAYRYFIQSAGLEAKLRE
LFADLDVEDVRNLQERGLKARSLLMHTPFPLELQQAIASAYESLCKQYNPDTDVAVRSSATAEDLPDASFAGQQETYLNV
VGAEAVLAACHKCFASLFTDRAISYRHTKGFDHFSVALAVGVQKMVRSDLATSGVMFSIDTETGFKDAALITAAYGLGEN
VVQGTVNPDEYCVFKPTLKTGFKPIIDKKLGSKELRMVYDEGSQSTKNEPVSIVEQNKFALSDADILQLAHWACLIEDHY
SQAHGSYTPMDIEWAKDGITNQLFVVQARPETVQSQKNGNVLRSYRLGLGTGEKTQNLSLIPLVTGRAIGEAISQGKARV
ILDAKQIEEFQAGEVLVTERTDPDWEPIMKRASAIITNSGGRTCHAAIIARELGVPAIVGCGKATQVLTTGQEVTVSCAE
GEEGKVYPGLLPFQVKEVLLENLPRTHTQILMNVGNPQEAFSLSAIPNDGVGLARTEFIIANQIQVHPLALIHYDNLADA
EQKAKVAAITAMYDDKPQYFVDKLAQGVGRIAAAFYPKPVIVRMSDFKSNEYRNLAGGSQFEPDEENPMLGWRGGCTLLR
SRL
>Mature_643_residues
MVTVSQNTLAKFSQEESLVLWLDKVGIKDIALVGGKNASLGEMIQQLTPKGVNVPTGFATTAYAYRYFIQSAGLEAKLRE
LFADLDVEDVRNLQERGLKARSLLMHTPFPLELQQAIASAYESLCKQYNPDTDVAVRSSATAEDLPDASFAGQQETYLNV
VGAEAVLAACHKCFASLFTDRAISYRHTKGFDHFSVALAVGVQKMVRSDLATSGVMFSIDTETGFKDAALITAAYGLGEN
VVQGTVNPDEYCVFKPTLKTGFKPIIDKKLGSKELRMVYDEGSQSTKNEPVSIVEQNKFALSDADILQLAHWACLIEDHY
SQAHGSYTPMDIEWAKDGITNQLFVVQARPETVQSQKNGNVLRSYRLGLGTGEKTQNLSLIPLVTGRAIGEAISQGKARV
ILDAKQIEEFQAGEVLVTERTDPDWEPIMKRASAIITNSGGRTCHAAIIARELGVPAIVGCGKATQVLTTGQEVTVSCAE
GEEGKVYPGLLPFQVKEVLLENLPRTHTQILMNVGNPQEAFSLSAIPNDGVGLARTEFIIANQIQVHPLALIHYDNLADA
EQKAKVAAITAMYDDKPQYFVDKLAQGVGRIAAAFYPKPVIVRMSDFKSNEYRNLAGGSQFEPDEENPMLGWRGGCTLLR
SRL

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=620, Percent_Identity=52.5806451612903, Blast_Score=640, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17564524, Length=243, Percent_Identity=29.2181069958848, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 70163; Mature: 70163

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVSQNTLAKFSQEESLVLWLDKVGIKDIALVGGKNASLGEMIQQLTPKGVNVPTGFAT
CEEECCHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHH
TAYAYRYFIQSAGLEAKLRELFADLDVEDVRNLQERGLKARSLLMHTPFPLELQQAIASA
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHH
YESLCKQYNPDTDVAVRSSATAEDLPDASFAGQQETYLNVVGAEAVLAACHKCFASLFTD
HHHHHHHCCCCCCEEEECCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAISYRHTKGFDHFSVALAVGVQKMVRSDLATSGVMFSIDTETGFKDAALITAAYGLGEN
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCH
VVQGTVNPDEYCVFKPTLKTGFKPIIDKKLGSKELRMVYDEGSQSTKNEPVSIVEQNKFA
HEECCCCCCCEEEECCCHHHCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCCEE
LSDADILQLAHWACLIEDHYSQAHGSYTPMDIEWAKDGITNQLFVVQARPETVQSQKNGN
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEECCHHHHHCCCCC
VLRSYRLGLGTGEKTQNLSLIPLVTGRAIGEAISQGKARVILDAKQIEEFQAGEVLVTER
EEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHCCCEEEEEEHHHHHHCCCCCEEEECC
TDPDWEPIMKRASAIITNSGGRTCHAAIIARELGVPAIVGCGKATQVLTTGQEVTVSCAE
CCCCHHHHHHHHHEEEECCCCCEEHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEEECC
GEEGKVYPGLLPFQVKEVLLENLPRTHTQILMNVGNPQEAFSLSAIPNDGVGLARTEFII
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHEEECCCCHHHEEEECCCCCCCCCEEEEEEE
ANQIQVHPLALIHYDNLADAEQKAKVAAITAMYDDKPQYFVDKLAQGVGRIAAAFYPKPV
ECCEEEEEEEEEEECCCCCHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCE
IVRMSDFKSNEYRNLAGGSQFEPDEENPMLGWRGGCTLLRSRL
EEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHCC
>Mature Secondary Structure
MVTVSQNTLAKFSQEESLVLWLDKVGIKDIALVGGKNASLGEMIQQLTPKGVNVPTGFAT
CEEECCHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHH
TAYAYRYFIQSAGLEAKLRELFADLDVEDVRNLQERGLKARSLLMHTPFPLELQQAIASA
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHH
YESLCKQYNPDTDVAVRSSATAEDLPDASFAGQQETYLNVVGAEAVLAACHKCFASLFTD
HHHHHHHCCCCCCEEEECCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAISYRHTKGFDHFSVALAVGVQKMVRSDLATSGVMFSIDTETGFKDAALITAAYGLGEN
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCH
VVQGTVNPDEYCVFKPTLKTGFKPIIDKKLGSKELRMVYDEGSQSTKNEPVSIVEQNKFA
HEECCCCCCCEEEECCCHHHCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCCEE
LSDADILQLAHWACLIEDHYSQAHGSYTPMDIEWAKDGITNQLFVVQARPETVQSQKNGN
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEECCHHHHHCCCCC
VLRSYRLGLGTGEKTQNLSLIPLVTGRAIGEAISQGKARVILDAKQIEEFQAGEVLVTER
EEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHCCCEEEEEEHHHHHHCCCCCEEEECC
TDPDWEPIMKRASAIITNSGGRTCHAAIIARELGVPAIVGCGKATQVLTTGQEVTVSCAE
CCCCHHHHHHHHHEEEECCCCCEEHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEEECC
GEEGKVYPGLLPFQVKEVLLENLPRTHTQILMNVGNPQEAFSLSAIPNDGVGLARTEFII
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHEEECCCCHHHEEEECCCCCCCCCEEEEEEE
ANQIQVHPLALIHYDNLADAEQKAKVAAITAMYDDKPQYFVDKLAQGVGRIAAAFYPKPV
ECCEEEEEEEEEEECCCCCHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCE
IVRMSDFKSNEYRNLAGGSQFEPDEENPMLGWRGGCTLLRSRL
EEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]