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The map label for this gene is pleD [H]

Identifier: 17230613

GI number: 17230613

Start: 3779784

End: 3780932

Strand: Direct

Name: pleD [H]

Synonym: alr3121

Alternate gene names: 17230613

Gene position: 3779784-3780932 (Clockwise)

Preceding gene: 17230612

Following gene: 17230614

Centisome position: 58.93

GC content: 42.91

Gene sequence:

>1149_bases
ATGAATCTGCCCTCTATTCTCGTAATTGACGATCTCCCAGATAACTTTGATGTTATTGAAACCCTCTTAAGTGAGCAAGA
CTATACACTACATTATGTTGCTAGTGGTCAAGAGGCGATCGCCTCCCTCAACTTATTTCAGCCAGACCTGATTTTGCTAG
ATGTGATGATGCCGGGAATGGATGGGATAGAAGTGTGCCAGCGAATTAAAGCCATGCCCCAATGGCAGCCAGTGCCAATC
ATTATGGTAACAGCCCTCACCGCCAAAGAAGACCTTGCACGCTGCTTACAATCAGGTGCCGATGACTTCATTAGTAAACC
TATCAATGCTCTGGAACTGAGGGCGCGCCTGCATTCCATGCTGAGGATTAAGCAACAGTATGACAAAATCCAAACTTTAT
CAAATCTCCAAACCAGCACCATCAAAGTCTTGGAAAGCACACTTGATGAACTGCGCGGCAATTTAGCTGCTACTTTACCC
CATGAACTCAATACACCACTAAATGGTATCGTTGGAAGCATCAGCATACTCATGGCAAATATTGAGAACATGGACACTGC
TGAAATCCAGGAACTTTTAGGCTGGGTAGATTTATCTGCCCGTAACCTAGAAAGATTAATCAAACAATTGCTGACCTATT
TAGAACTGGAATTATCAACAAACCAACAGCAAAACATTGCATCTGAGTCAACCCACTTTTCCATAGCAGCAATTGAAGCA
ATTTTGCAATCTCATGCTCCCAGTGTTAATCGCCGCGACGATCTCATGTTTGCAATTGAGGAAGCTGAGGTGTCAATCTC
GGCTCGATATCTAACGATTATCATCCATGAATTAGTTGATAATGCGCTCAAATTTTCCCGGAGAGGGACAACCATTAAAA
TCAGTAGTCAGATAGTAGCAGGAATGTTAAATGTCTACATTCATGATTTCGGACGGGGGATGACAGCAGAACAGATATCT
AAAATCGGCGCTTTTATGCAGTTTGAACGCAAAACTTATGAACAGCAAGGTATAGGCATGGGCTTAAAGCTTGCCAAACA
AACCGTCGAAAGTTATGGGGGATTATTCTCAATTTCCAGTGTCTATCAACAGGAAACAACGGTACACATTGCCTTACCCA
TTGCCTATTCTAGACACTCAAGTTGGTGA

Upstream 100 bases:

>100_bases
ATCGCTGTTTACAAGCCGGAGCCAATGACTACCTAACTAAACCCGTGAAGCTCAAGCAACTGGCGACCACTATTCAACAG
CTTTTAATTACAACAAAAAA

Downstream 100 bases:

>100_bases
TCAACTAATGCGATTCCATAGGGCTTGTAGGTTCTCGACGTTAACGGTGAACCAGTTGGCTGAATACCAAGTTTTCTCAC
TATGAAGACAAACATCAATC

Product: two-component hybrid sensor and regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNLPSILVIDDLPDNFDVIETLLSEQDYTLHYVASGQEAIASLNLFQPDLILLDVMMPGMDGIEVCQRIKAMPQWQPVPI
IMVTALTAKEDLARCLQSGADDFISKPINALELRARLHSMLRIKQQYDKIQTLSNLQTSTIKVLESTLDELRGNLAATLP
HELNTPLNGIVGSISILMANIENMDTAEIQELLGWVDLSARNLERLIKQLLTYLELELSTNQQQNIASESTHFSIAAIEA
ILQSHAPSVNRRDDLMFAIEEAEVSISARYLTIIIHELVDNALKFSRRGTTIKISSQIVAGMLNVYIHDFGRGMTAEQIS
KIGAFMQFERKTYEQQGIGMGLKLAKQTVESYGGLFSISSVYQQETTVHIALPIAYSRHSSW

Sequences:

>Translated_382_residues
MNLPSILVIDDLPDNFDVIETLLSEQDYTLHYVASGQEAIASLNLFQPDLILLDVMMPGMDGIEVCQRIKAMPQWQPVPI
IMVTALTAKEDLARCLQSGADDFISKPINALELRARLHSMLRIKQQYDKIQTLSNLQTSTIKVLESTLDELRGNLAATLP
HELNTPLNGIVGSISILMANIENMDTAEIQELLGWVDLSARNLERLIKQLLTYLELELSTNQQQNIASESTHFSIAAIEA
ILQSHAPSVNRRDDLMFAIEEAEVSISARYLTIIIHELVDNALKFSRRGTTIKISSQIVAGMLNVYIHDFGRGMTAEQIS
KIGAFMQFERKTYEQQGIGMGLKLAKQTVESYGGLFSISSVYQQETTVHIALPIAYSRHSSW
>Mature_382_residues
MNLPSILVIDDLPDNFDVIETLLSEQDYTLHYVASGQEAIASLNLFQPDLILLDVMMPGMDGIEVCQRIKAMPQWQPVPI
IMVTALTAKEDLARCLQSGADDFISKPINALELRARLHSMLRIKQQYDKIQTLSNLQTSTIKVLESTLDELRGNLAATLP
HELNTPLNGIVGSISILMANIENMDTAEIQELLGWVDLSARNLERLIKQLLTYLELELSTNQQQNIASESTHFSIAAIEA
ILQSHAPSVNRRDDLMFAIEEAEVSISARYLTIIIHELVDNALKFSRRGTTIKISSQIVAGMLNVYIHDFGRGMTAEQIS
KIGAFMQFERKTYEQQGIGMGLKLAKQTVESYGGLFSISSVYQQETTVHIALPIAYSRHSSW

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=39.8305084745763, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788394, Length=121, Percent_Identity=35.5371900826446, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=35.042735042735, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789149, Length=117, Percent_Identity=34.1880341880342, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082012, Length=136, Percent_Identity=31.6176470588235, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786911, Length=76, Percent_Identity=44.7368421052632, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=37.8640776699029, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789402, Length=117, Percent_Identity=31.6239316239316, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=29.1666666666667, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788550, Length=128, Percent_Identity=27.34375, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=32.7586206896552, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42661; Mature: 42661

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPSILVIDDLPDNFDVIETLLSEQDYTLHYVASGQEAIASLNLFQPDLILLDVMMPGM
CCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHCCCC
DGIEVCQRIKAMPQWQPVPIIMVTALTAKEDLARCLQSGADDFISKPINALELRARLHSM
CHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHH
LRIKQQYDKIQTLSNLQTSTIKVLESTLDELRGNLAATLPHELNTPLNGIVGSISILMAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCHHHHHHHHHHHHHH
IENMDTAEIQELLGWVDLSARNLERLIKQLLTYLELELSTNQQQNIASESTHFSIAAIEA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHH
ILQSHAPSVNRRDDLMFAIEEAEVSISARYLTIIIHELVDNALKFSRRGTTIKISSQIVA
HHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
GMLNVYIHDFGRGMTAEQISKIGAFMQFERKTYEQQGIGMGLKLAKQTVESYGGLFSISS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEHHHH
VYQQETTVHIALPIAYSRHSSW
HHHCCCEEEEEEEEEECCCCCC
>Mature Secondary Structure
MNLPSILVIDDLPDNFDVIETLLSEQDYTLHYVASGQEAIASLNLFQPDLILLDVMMPGM
CCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHCCCC
DGIEVCQRIKAMPQWQPVPIIMVTALTAKEDLARCLQSGADDFISKPINALELRARLHSM
CHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHH
LRIKQQYDKIQTLSNLQTSTIKVLESTLDELRGNLAATLPHELNTPLNGIVGSISILMAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCHHHHHHHHHHHHHH
IENMDTAEIQELLGWVDLSARNLERLIKQLLTYLELELSTNQQQNIASESTHFSIAAIEA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHH
ILQSHAPSVNRRDDLMFAIEEAEVSISARYLTIIIHELVDNALKFSRRGTTIKISSQIVA
HHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
GMLNVYIHDFGRGMTAEQISKIGAFMQFERKTYEQQGIGMGLKLAKQTVESYGGLFSISS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEHHHH
VYQQETTVHIALPIAYSRHSSW
HHHCCCEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA