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The map label for this gene is hisF

Identifier: 17230387

GI number: 17230387

Start: 3534050

End: 3534829

Strand: Direct

Name: hisF

Synonym: alr2895

Alternate gene names: 17230387

Gene position: 3534050-3534829 (Clockwise)

Preceding gene: 17230384

Following gene: 17230388

Centisome position: 55.1

GC content: 45.51

Gene sequence:

>780_bases
ATGTTATCTAAAAGAATCTTACCTTGTTTAGATGTGAAGGCGGGACGGGTAGTTAAAGGAGTTAACTTTGTTGATCTTAA
GGATGCTGGCGATCCTGTAGAACTGGCGAAGGTTTACAACGATGCTGGGGCTGATGAGTTAGTGTTTCTGGATATTACGG
CTACTCATGAAGACCGAGATACTATCATTGACGTTGTTTACCGGACTGCCGAGCAGGTCTTTATTCCCCTGACTGTAGGT
GGTGGTATTCAATCCTTAGAAAATGTTAAAGCTTTGTTACGCGCTGGGGCAGACAAGGTTAGTATTAACTCTGCGGCGGT
ACGTGACCCAGAATTAATTGATCGAGCGAGCGATCACTTTGGTAATCAGTGCATTGTTGTAGCAATTGATGCTAGACGCA
GAGTAGATCCTAGTAATCCCGGCTGGGATGTTTACGTGCGTGGTGGTAGGGAAAACACTGGTTTAGATGCCTTATCATGG
GCGAAGGAAGTAGAAAAGCGGGGTGCAGGAGAACTATTGGTAACAAGTATGGATGCTGATGGTACTCAAGCCGGTTATGA
TATCGAGTTAACACGGGCAATTGCTGAGTCAGTAGAAATACCAGTCGTTGCCTCCGGTGGTGCGGGTAACTGTGAACATA
TATACACTGCCTTAACTGAAGGGAAAGCGGAAGCTGCGTTATTGGCATCGCTGCTACATTATGGTCAACTCAGCGTGGCT
GAAATTAAGAATTATTTGCACGAGAGCCAAGTTCCTGTCCGCTTATACACACAGTTCTAA

Upstream 100 bases:

>100_bases
TACTGGTAGTAGAAATGGCGGCGAAGATTTTAGTGAGAAAATACACAGGTCAATTTAAAATTTAAATTCCGGACAATTAC
CCAGGAGTAAAAAAATTATT

Downstream 100 bases:

>100_bases
AGACATCATGGAATTTACATTACGTCTAGCACAAGTCGGGTACAGTTCTCGAAAAGGATGTTAAGATTGTTTTACAAGTA
TTAATAAATATTAAAAAATA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLSKRILPCLDVKAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRDTIIDVVYRTAEQVFIPLTVG
GGIQSLENVKALLRAGADKVSINSAAVRDPELIDRASDHFGNQCIVVAIDARRRVDPSNPGWDVYVRGGRENTGLDALSW
AKEVEKRGAGELLVTSMDADGTQAGYDIELTRAIAESVEIPVVASGGAGNCEHIYTALTEGKAEAALLASLLHYGQLSVA
EIKNYLHESQVPVRLYTQF

Sequences:

>Translated_259_residues
MLSKRILPCLDVKAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRDTIIDVVYRTAEQVFIPLTVG
GGIQSLENVKALLRAGADKVSINSAAVRDPELIDRASDHFGNQCIVVAIDARRRVDPSNPGWDVYVRGGRENTGLDALSW
AKEVEKRGAGELLVTSMDADGTQAGYDIELTRAIAESVEIPVVASGGAGNCEHIYTALTEGKAEAALLASLLHYGQLSVA
EIKNYLHESQVPVRLYTQF
>Mature_259_residues
MLSKRILPCLDVKAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRDTIIDVVYRTAEQVFIPLTVG
GGIQSLENVKALLRAGADKVSINSAAVRDPELIDRASDHFGNQCIVVAIDARRRVDPSNPGWDVYVRGGRENTGLDALSW
AKEVEKRGAGELLVTSMDADGTQAGYDIELTRAIAESVEIPVVASGGAGNCEHIYTALTEGKAEAALLASLLHYGQLSVA
EIKNYLHESQVPVRLYTQF

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=45.5598455598456, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=33.7579617834395, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_NOSS1 (Q8YT31)

Other databases:

- EMBL:   BA000019
- PIR:   AH2167
- RefSeq:   NP_486935.1
- ProteinModelPortal:   Q8YT31
- SMR:   Q8YT31
- STRING:   Q8YT31
- GeneID:   1106493
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr2895
- NMPDR:   fig|103690.1.peg.3202
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q8YT31
- ProtClustDB:   PRK02083
- BioCyc:   NSP103690:ALR2895-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27919; Mature: 27919

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKRILPCLDVKAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRD
CCCCCCCCCCCCCCCCEEECCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHH
TIIDVVYRTAEQVFIPLTVGGGIQSLENVKALLRAGADKVSINSAAVRDPELIDRASDHF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHC
GNQCIVVAIDARRRVDPSNPGWDVYVRGGRENTGLDALSWAKEVEKRGAGELLVTSMDAD
CCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCC
GTQAGYDIELTRAIAESVEIPVVASGGAGNCEHIYTALTEGKAEAALLASLLHYGQLSVA
CCCCCCCEEHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
EIKNYLHESQVPVRLYTQF
HHHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MLSKRILPCLDVKAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRD
CCCCCCCCCCCCCCCCEEECCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHH
TIIDVVYRTAEQVFIPLTVGGGIQSLENVKALLRAGADKVSINSAAVRDPELIDRASDHF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHC
GNQCIVVAIDARRRVDPSNPGWDVYVRGGRENTGLDALSWAKEVEKRGAGELLVTSMDAD
CCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCC
GTQAGYDIELTRAIAESVEIPVVASGGAGNCEHIYTALTEGKAEAALLASLLHYGQLSVA
CCCCCCCEEHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
EIKNYLHESQVPVRLYTQF
HHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840