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The map label for this gene is wcaJ [H]

Identifier: 17230346

GI number: 17230346

Start: 3475866

End: 3477275

Strand: Reverse

Name: wcaJ [H]

Synonym: all2854

Alternate gene names: 17230346

Gene position: 3477275-3475866 (Counterclockwise)

Preceding gene: 17230347

Following gene: 17230345

Centisome position: 54.22

GC content: 42.13

Gene sequence:

>1410_bases
ATGGTTATTACTCTTACTGCTGGAAAAAAAATCAGTAATTCACATCAGACCCGTAATATTCTCTGTGTATTGCTGCTGAT
AGGAGATATTTTGGGTTTGGTGTTGAGTTATTCCATGTGTCTATGGTTGAGATTGGGTAAGAATTTGCATGGTTTTGACC
CGTTAATTTATGTATTTTTCTTTCTAGTTCTCGCAGGGTTGTATTTAGCAGATACCTACCATCCAGATAGACAAATTGCA
GGTTTACGCGCCCCATCCCGCATTTTGATTAGTAATTTTGTAGTTGGCGCTATCATTGCAGCACTCATTTACTGTACCAG
TGCTTGGGGAACAGACTTGCTTCTGAAACGGGGAATTTGGCTTCCTAGCTTAGGGCTATTTACTATCTGGGCTATGTGGT
TAAGAATAGGAGCAGCAAACTGGGCAAAAACCCAAGCTCAACACAGTCGTTGGTTAATTTTGGGCGCAGGTAAAAAAGCC
ATCCTCTTTGGCAAAACTTTTCTCGAACGCAGTTCGTTGGGGCGCTTGATTTTTCTCACAACAGCAGAGCAGAACAGCAA
TGAGTTAACAGAAAATCATCGAGTTTCTGTAGGAAGTCTGAATGACTTATCCGAATGGAGCCAACAACCTTGGTCAGGGG
TAGTAGTGACGACTCCAACGGAGTTATCTGATGTACAAATGCAACAACTGATGCAGTTGCGTTTATCTGGGGTTTCCATT
TACGGTCTTCCCGATGTTTGTGAAACCTTGTGGTATAAACTTCCCTCATCTCTGCTTGAGGATAATTGGCTGGCTTTTGG
TAATGGTTTTAATTTAACTGCTGACAGCATTAGTCAAAAGCTCAAGCGCTCAGTAGATATTATGCTGACGTGCTGTTTAT
GTGTGTTTTTATCTCCATTGATGTTGTTGGCAGCTTTAGCAATTAAGTTAGATAGCCCTGGCCCGGTGTTCTACTCTCAA
ATACGCACTGGACTAGAAGGTAAGCCTTTTAAAGTTTACAAATTTCGCTCCATGTATCAAGATGCCGAAAAACGTGGGGC
GCAGTGGGCAGATGAACGAGACCCACGAATTACCAGAGTAGGGCGTTGGTTACGCCTAACTAGAATTGATGAATTACCAC
AGATATTAAACGTTATCTGGGGAGAAATGAGCCTTATTGGCCCTCGTCCAGAAAGACCAGAATTTGATGTCAAACTGCGT
CAAGAAATTCCCTATTATGATTTGCGTTATGTCGTCAAACCTGGTATTACAGGCTGGGCGCAGGTCATGTATCCCTACGG
CGCATCAATTGAAGATGCTTACGAAAAGCTAGCTTATGACCTCTACTACATCAAGAATTACTCCCTAGCTTTAGATTTAG
CGATCGCTCTCAAAACTATTCGAGTAGTATTATTAGGTAAAGGTAGATGA

Upstream 100 bases:

>100_bases
GTATTTGAGATTTTCACATCTACCGCTAAGAGTTCCAGAGTAATTACTTTTTGTATCTCATCTTATCTGTAATCTTGGAA
ATTTTTAGTAGCCTACATAC

Downstream 100 bases:

>100_bases
ATCAAAAAGTTTTAGTTACCGGTGGTGCTGGCTACATTGGTTCTCATGTGGTGCGCCAGCTAGGTGAAGCCGGCTACGAT
GTTGTTGTGTATGATAACTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MVITLTAGKKISNSHQTRNILCVLLLIGDILGLVLSYSMCLWLRLGKNLHGFDPLIYVFFFLVLAGLYLADTYHPDRQIA
GLRAPSRILISNFVVGAIIAALIYCTSAWGTDLLLKRGIWLPSLGLFTIWAMWLRIGAANWAKTQAQHSRWLILGAGKKA
ILFGKTFLERSSLGRLIFLTTAEQNSNELTENHRVSVGSLNDLSEWSQQPWSGVVVTTPTELSDVQMQQLMQLRLSGVSI
YGLPDVCETLWYKLPSSLLEDNWLAFGNGFNLTADSISQKLKRSVDIMLTCCLCVFLSPLMLLAALAIKLDSPGPVFYSQ
IRTGLEGKPFKVYKFRSMYQDAEKRGAQWADERDPRITRVGRWLRLTRIDELPQILNVIWGEMSLIGPRPERPEFDVKLR
QEIPYYDLRYVVKPGITGWAQVMYPYGASIEDAYEKLAYDLYYIKNYSLALDLAIALKTIRVVLLGKGR

Sequences:

>Translated_469_residues
MVITLTAGKKISNSHQTRNILCVLLLIGDILGLVLSYSMCLWLRLGKNLHGFDPLIYVFFFLVLAGLYLADTYHPDRQIA
GLRAPSRILISNFVVGAIIAALIYCTSAWGTDLLLKRGIWLPSLGLFTIWAMWLRIGAANWAKTQAQHSRWLILGAGKKA
ILFGKTFLERSSLGRLIFLTTAEQNSNELTENHRVSVGSLNDLSEWSQQPWSGVVVTTPTELSDVQMQQLMQLRLSGVSI
YGLPDVCETLWYKLPSSLLEDNWLAFGNGFNLTADSISQKLKRSVDIMLTCCLCVFLSPLMLLAALAIKLDSPGPVFYSQ
IRTGLEGKPFKVYKFRSMYQDAEKRGAQWADERDPRITRVGRWLRLTRIDELPQILNVIWGEMSLIGPRPERPEFDVKLR
QEIPYYDLRYVVKPGITGWAQVMYPYGASIEDAYEKLAYDLYYIKNYSLALDLAIALKTIRVVLLGKGR
>Mature_469_residues
MVITLTAGKKISNSHQTRNILCVLLLIGDILGLVLSYSMCLWLRLGKNLHGFDPLIYVFFFLVLAGLYLADTYHPDRQIA
GLRAPSRILISNFVVGAIIAALIYCTSAWGTDLLLKRGIWLPSLGLFTIWAMWLRIGAANWAKTQAQHSRWLILGAGKKA
ILFGKTFLERSSLGRLIFLTTAEQNSNELTENHRVSVGSLNDLSEWSQQPWSGVVVTTPTELSDVQMQQLMQLRLSGVSI
YGLPDVCETLWYKLPSSLLEDNWLAFGNGFNLTADSISQKLKRSVDIMLTCCLCVFLSPLMLLAALAIKLDSPGPVFYSQ
IRTGLEGKPFKVYKFRSMYQDAEKRGAQWADERDPRITRVGRWLRLTRIDELPQILNVIWGEMSLIGPRPERPEFDVKLR
QEIPYYDLRYVVKPGITGWAQVMYPYGASIEDAYEKLAYDLYYIKNYSLALDLAIALKTIRVVLLGKGR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=424, Percent_Identity=31.6037735849057, Blast_Score=172, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53041; Mature: 53041

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVITLTAGKKISNSHQTRNILCVLLLIGDILGLVLSYSMCLWLRLGKNLHGFDPLIYVFF
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FLVLAGLYLADTYHPDRQIAGLRAPSRILISNFVVGAIIAALIYCTSAWGTDLLLKRGIW
HHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
LPSLGLFTIWAMWLRIGAANWAKTQAQHSRWLILGAGKKAILFGKTFLERSSLGRLIFLT
CCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCEEEEEHHHHHHHHCCCCEEEEE
TAEQNSNELTENHRVSVGSLNDLSEWSQQPWSGVVVTTPTELSDVQMQQLMQLRLSGVSI
ECCCCCCCCCCCCCEECCCCHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEE
YGLPDVCETLWYKLPSSLLEDNWLAFGNGFNLTADSISQKLKRSVDIMLTCCLCVFLSPL
ECCHHHHHHHHHHCCHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLAALAIKLDSPGPVFYSQIRTGLEGKPFKVYKFRSMYQDAEKRGAQWADERDPRITRV
HHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHH
GRWLRLTRIDELPQILNVIWGEMSLIGPRPERPEFDVKLRQEIPYYDLRYVVKPGITGWA
HHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCHHHCCCCCHHEEEEECCCCCHHH
QVMYPYGASIEDAYEKLAYDLYYIKNYSLALDLAIALKTIRVVLLGKGR
HHHCCCCCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MVITLTAGKKISNSHQTRNILCVLLLIGDILGLVLSYSMCLWLRLGKNLHGFDPLIYVFF
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FLVLAGLYLADTYHPDRQIAGLRAPSRILISNFVVGAIIAALIYCTSAWGTDLLLKRGIW
HHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
LPSLGLFTIWAMWLRIGAANWAKTQAQHSRWLILGAGKKAILFGKTFLERSSLGRLIFLT
CCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCEEEEEHHHHHHHHCCCCEEEEE
TAEQNSNELTENHRVSVGSLNDLSEWSQQPWSGVVVTTPTELSDVQMQQLMQLRLSGVSI
ECCCCCCCCCCCCCEECCCCHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEE
YGLPDVCETLWYKLPSSLLEDNWLAFGNGFNLTADSISQKLKRSVDIMLTCCLCVFLSPL
ECCHHHHHHHHHHCCHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLAALAIKLDSPGPVFYSQIRTGLEGKPFKVYKFRSMYQDAEKRGAQWADERDPRITRV
HHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHH
GRWLRLTRIDELPQILNVIWGEMSLIGPRPERPEFDVKLRQEIPYYDLRYVVKPGITGWA
HHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCHHHCCCCCHHEEEEECCCCCHHH
QVMYPYGASIEDAYEKLAYDLYYIKNYSLALDLAIALKTIRVVLLGKGR
HHHCCCCCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]