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The map label for this gene is phoA [H]

Identifier: 17230335

GI number: 17230335

Start: 3463675

End: 3465558

Strand: Reverse

Name: phoA [H]

Synonym: all2843

Alternate gene names: 17230335

Gene position: 3465558-3463675 (Counterclockwise)

Preceding gene: 17230336

Following gene: 17230334

Centisome position: 54.03

GC content: 46.55

Gene sequence:

>1884_bases
GTGTTTGGCACACCAGGCGTTGAATCTGCCCTAGCGGCTGGTAATGGAATTAACGTCATCATCATGATTGGTGATGGGAT
GGGTTGGGAAATGGCCAGAGCCGCAGCCGTAGCTAAAGGCGCTCCCTTCTATACCAGTGGGAAAGGTTCTGGTCTGAGCT
TTCAAAAGCTAACTGGATATGGAATTGTGACAACCTACGGGACAACAGTCCAAGGTAGTACATCAACTAATCCAGATAAT
GAGACACGTTCAGCCAATAACTCGGCTCTAGATGGAAGCAATCCATTAACTGGCGCAAGTCCTGTGCGTCCCAACTTCTC
ATTCTCACCTGCTCCTTTTAACCCAGGCGATCGCGCTGATGGTAACAGTCCAGCACCCGGTAACTTGGCTGGTTATGACC
CCAGCAAAGGCGGCCCTGTACCTTGGATACCTCTATCTCCCGCTAATCCTGGTAACTACGATAAAGAGTACATCAAATAT
AGCTATCCCGACTCTGCAAACACCGCTACAACTCTATACACAGGTGTTAAGAGCTATAACAACGCAATGGGGGTAGATAT
TTACGAGCAGAAGTTAAAAACTATCCTAGAAATTGCTAAAGAAGAGGGCAAAGCTACAGGGCTAGTAACTTCTGTACCCA
TCACCCACGCTACACCAGGGGCTGCGGCTTCTTACGTCAACCGTCGTAGCAAGTACGACAGTGTTTATGACCCTACCAAG
ACCAACCAAGACAGCATCTTACAACAGATGTTGCTAGACTTTCAGCCCAACATATTGTTAGGTGGTGGTCATCCTCTCGA
TATGCAAAATAGAGGCGCGACAGGCCCAGTTAATAACTACACCTTCATCACCCAAGACACTTACGAACATCTCAAAAATA
ACCCCACTCCCACATCTAACCGTTATGGTTATACCTTCTTGGAGCGTGGGCCTAATGCTACTCAAACATTACTAAACACA
GCAGCTCAAATTGATCCCAATAGAGGCGATAAACTCTTGGGTCTATACGGCGCTCGTGGACAAAACGGTAATATTCCTCT
AAGTTCTTCCAAGGGAGACTACAGCACCACAGGTTTAGATAACTTCTCTGTCTATAGCACTGTTGTGAGAGGTGGAACCC
CCGCTACTTGCCCATCAGGACAAATCATTCCTGGCTCTATCAGTGAGTGTGTACCCGTGCTGGATGGCAATGGTAATCCT
GTGACTGGCCCCACACCTGATAGAGTCCGTCCCCTCGCTCCCGGTGAAACCGATGCTAGTTTCATCGCCAGAGAAATTAA
CGAAAACCCTACCTTGGCTGATTTAAGCAAGGCTGCTCTCAATGTCTTAGGTAAAGACAAAGATGGCTTTTGGTTAATGA
TTGAGGGTGGTGATATTGACTGGTCTGCTCATGATGACAACATGGATAACCTGATTGGAACCATGAATGACTTTGATAAA
GCAGTCCAAGAGGTGATCAGTTGGATTAACAAAAATGGTGGTTGGAGTAAGAACCTGTTAATCGTGACAGCTGACCATGA
CCATTATTTGACTCTAAATAATGACTTTGTTTCTAAGCTGACTCCTAATCCCCACCCCAATCAATCCAGAGGCTTGAAGT
ATAACGCCAAAGAAATTACTTACAACAAGCACAACCCTCAAGATGCGGGACACTTCTGGGGTTCTGATCCCACTCAGAAA
TACCTCTGGGGAAGTCACAGCCGAAGACCTGTCCCTGTTTATTTCCAAGGTGCTTATTCTTCCAACCTCGTGAGATATTT
TGGTCAACCGGTGCAGTTCACAGATAGTTCTGGTTCCTACACTGCATCTGGTATTCGCAGTGTTGTTGATCAAAGCCACA
TCTTCCAAGCCATGAAAGCTGCATTAACTCAGCCACCACTCTAG

Upstream 100 bases:

>100_bases
TTAATTAACTGCAAGGCGCACTGCAATGATTTCACTTTTGGAAAGATGTCGGCGAGCGTTCAGCTTTGCTATGGCTTGTT
TTTTCTGCACTATGGGGCTA

Downstream 100 bases:

>100_bases
TGGAGAGTTTACATAGCTCATATATATTCTGTAAATCAAGGAAAAAAATGACTAGCTCTATCCTCAAAAAAGTAGCAGGT
GTAACAGTAGGAACTACTTT

Product: alkaline phosphatase

Products: NA

Alternate protein names: High molecular weight phosphatase; H-AP [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGYGIVTTYGTTVQGSTSTNPDN
ETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRADGNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKY
SYPDSANTATTLYTGVKSYNNAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK
TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSNRYGYTFLERGPNATQTLLNT
AAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLDNFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNP
VTGPTPDRVRPLAPGETDASFIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK
AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEITYNKHNPQDAGHFWGSDPTQK
YLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSYTASGIRSVVDQSHIFQAMKAALTQPPL

Sequences:

>Translated_627_residues
MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGYGIVTTYGTTVQGSTSTNPDN
ETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRADGNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKY
SYPDSANTATTLYTGVKSYNNAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK
TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSNRYGYTFLERGPNATQTLLNT
AAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLDNFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNP
VTGPTPDRVRPLAPGETDASFIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK
AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEITYNKHNPQDAGHFWGSDPTQK
YLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSYTASGIRSVVDQSHIFQAMKAALTQPPL
>Mature_627_residues
MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGYGIVTTYGTTVQGSTSTNPDN
ETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRADGNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKY
SYPDSANTATTLYTGVKSYNNAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK
TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSNRYGYTFLERGPNATQTLLNT
AAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLDNFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNP
VTGPTPDRVRPLAPGETDASFIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK
AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEITYNKHNPQDAGHFWGSDPTQK
YLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSYTASGIRSVVDQSHIFQAMKAALTQPPL

Specific function: Has only a phosphomonoesterase activity [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted. Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI48994877, Length=112, Percent_Identity=41.0714285714286, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 67221; Mature: 67221

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGY
CCCCCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEECCCCCCCCHHHCCCC
GIVTTYGTTVQGSTSTNPDNETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRAD
EEEEECCCEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKYSYPDSANTATTLYTGVKSYN
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHC
NAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCC
TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSN
CCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCC
RYGYTFLERGPNATQTLLNTAAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLD
CCCEEHEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
NFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNPVTGPTPDRVRPLAPGETDAS
CHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHH
FIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEIT
HHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHCCCCCCCCCCCCCEECCCEEE
YNKHNPQDAGHFWGSDPTQKYLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSY
ECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHCCCCEEEECCCCCE
TASGIRSVVDQSHIFQAMKAALTQPPL
EHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGY
CCCCCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEECCCCCCCCHHHCCCC
GIVTTYGTTVQGSTSTNPDNETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRAD
EEEEECCCEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKYSYPDSANTATTLYTGVKSYN
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHC
NAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCC
TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSN
CCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCC
RYGYTFLERGPNATQTLLNTAAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLD
CCCEEHEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
NFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNPVTGPTPDRVRPLAPGETDAS
CHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHH
FIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEIT
HHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHCCCCCCCCCCCCCEECCCEEE
YNKHNPQDAGHFWGSDPTQKYLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSY
ECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHCCCCEEEECCCCCE
TASGIRSVVDQSHIFQAMKAALTQPPL
EHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 8454193 [H]