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The map label for this gene is cobQ

Identifier: 17229869

GI number: 17229869

Start: 2858433

End: 2859914

Strand: Direct

Name: cobQ

Synonym: alr2377

Alternate gene names: 17229869

Gene position: 2858433-2859914 (Clockwise)

Preceding gene: 17229868

Following gene: 17229870

Centisome position: 44.57

GC content: 46.15

Gene sequence:

>1482_bases
GTGGCTATGAAATCAATAATGGTAGTGGGAACAACATCCCACGCTGGAAAATCACTGATAAGTACGGCTATTTGTCGCAT
TTTGTCACGGCGTGGTTGGCGAGTAGCACCCTTCAAGGGGCAAAATATGGCATTAAATGCCTATGTCACAGCCAATGGGG
GAGAAATTGGCTACGCACAAGCAGTACAAGCCTGGGCAGCAGGGGTGGTACCGTGGGTAGAAATGAATCCAATTCTACTC
AAACCTCAAGGAGATATGACTTCTCAAGTCATTATTAGAGGTAGACCTGTAGGCAGAGTCAATGCTGCTGATTATTATGA
GCAGTATTTTGAACCTGGTTGGCGGGCAATTGAAGAATCTCTACAACATTTAGCAACCGAATTTGATTTAGTCGTTTGTG
AAGGCGCGGGTAGTCCAGCAGAAATCAACCTTAAGCACCGCGACTTAACTAATATGCGGGTAGCAAAATATTTGAATGCC
CCAACTCTATTAGTAGTTGATATTGACCGTGGTGGTGCTTTTGCTCACGTAGTCGGCACACTAGAATTATTAGACCCAGA
AGAACGCCAACTAATTAAAGGTGTAGTCATTAACAAATTCCGGGGACAGCGATCGCTTCTAGACCCAGGAATCAAATGGT
TAGAAGAACGGACTGGTATTCCTGTGGTCGGTGTAATTCCCTACTTACAAGAAGTTTTCCCCGCCGAAGATTCCCTAGAC
TTGTTAGAACGCAAAACACACAAAGCCCACGCAGATCTCCAAATCACAGTTGTTCGCTTACCGCGAATTGCCAACTTTAC
CGACTTCGACCCTCTAGAATCAGAACCCACCGTTGCGGTCAAATACATTAGCCCGAAACAAGATTTAGGACACCCAGATG
CAGTAATTTTGCCAGGGACAAAAACTACAATTGCCGACTTGATATTATTGCAAAAGACTGGCATGGCAGAAGCAATCCAG
AACTACGCAGCCTCCGGGGGTACAGTTTTGGGTATTTGCGGTGGCTACCAAATTCTGGGGCAAATGATCGCCGATCCAGA
AGGTATCGAAGGCCAAGCTGGTAGATATCAGGGCTTGAATCTCTTACCAATTAGAACCGTAATCACCGGACAAAAGATTG
CTCGTCAGCGCCAAGTTAGCTCCAATTTCCCGCAACAGGGTTTACCAGTCAACGGCTTTGAGATTCACCAAGGGCGATCG
CGCGTGGAACCACAAGGCGATAGCCAAGCTTTCCAACCTCTATTCGATGACGTTAACTTAGGCTTAGTAGATAGCTGTCA
ATCTGTTTGGGGTAGTTATCTACACGGATTGTTTGACAATGGCCCCTGGCGGCGTGCTTGGTTAAATCGTCTTCGTCAAC
AGCGCGGTTTAAAATCTCTACCTACAGGTGTGGCTAACTACCGCGAACAACGAGAGCAGATGTTAGATAATATAGCTACT
GAAGTAGAAAACCATTTAGACTTGACCCCCTTTCTGCCCTAG

Upstream 100 bases:

>100_bases
CATCAATTTACTTCAGTAGCTGCCCAAACTTCTGACATTTGAGCAGTAAATTGCTATATTATCCCCCAACTATCTCACCC
CTTATTTTGAATAACTAAAA

Downstream 100 bases:

>100_bases
CGAGGCTAGTATCTCATGAATGTTTGCGTCCGATTCTTGCCAGATGATGTCACCACTAATGCTGAAGTGGGAGAAGCCCT
ATTAGATGTAGCAGATCGGG

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MAMKSIMVVGTTSHAGKSLISTAICRILSRRGWRVAPFKGQNMALNAYVTANGGEIGYAQAVQAWAAGVVPWVEMNPILL
KPQGDMTSQVIIRGRPVGRVNAADYYEQYFEPGWRAIEESLQHLATEFDLVVCEGAGSPAEINLKHRDLTNMRVAKYLNA
PTLLVVDIDRGGAFAHVVGTLELLDPEERQLIKGVVINKFRGQRSLLDPGIKWLEERTGIPVVGVIPYLQEVFPAEDSLD
LLERKTHKAHADLQITVVRLPRIANFTDFDPLESEPTVAVKYISPKQDLGHPDAVILPGTKTTIADLILLQKTGMAEAIQ
NYAASGGTVLGICGGYQILGQMIADPEGIEGQAGRYQGLNLLPIRTVITGQKIARQRQVSSNFPQQGLPVNGFEIHQGRS
RVEPQGDSQAFQPLFDDVNLGLVDSCQSVWGSYLHGLFDNGPWRRAWLNRLRQQRGLKSLPTGVANYREQREQMLDNIAT
EVENHLDLTPFLP

Sequences:

>Translated_493_residues
MAMKSIMVVGTTSHAGKSLISTAICRILSRRGWRVAPFKGQNMALNAYVTANGGEIGYAQAVQAWAAGVVPWVEMNPILL
KPQGDMTSQVIIRGRPVGRVNAADYYEQYFEPGWRAIEESLQHLATEFDLVVCEGAGSPAEINLKHRDLTNMRVAKYLNA
PTLLVVDIDRGGAFAHVVGTLELLDPEERQLIKGVVINKFRGQRSLLDPGIKWLEERTGIPVVGVIPYLQEVFPAEDSLD
LLERKTHKAHADLQITVVRLPRIANFTDFDPLESEPTVAVKYISPKQDLGHPDAVILPGTKTTIADLILLQKTGMAEAIQ
NYAASGGTVLGICGGYQILGQMIADPEGIEGQAGRYQGLNLLPIRTVITGQKIARQRQVSSNFPQQGLPVNGFEIHQGRS
RVEPQGDSQAFQPLFDDVNLGLVDSCQSVWGSYLHGLFDNGPWRRAWLNRLRQQRGLKSLPTGVANYREQREQMLDNIAT
EVENHLDLTPFLP
>Mature_492_residues
AMKSIMVVGTTSHAGKSLISTAICRILSRRGWRVAPFKGQNMALNAYVTANGGEIGYAQAVQAWAAGVVPWVEMNPILLK
PQGDMTSQVIIRGRPVGRVNAADYYEQYFEPGWRAIEESLQHLATEFDLVVCEGAGSPAEINLKHRDLTNMRVAKYLNAP
TLLVVDIDRGGAFAHVVGTLELLDPEERQLIKGVVINKFRGQRSLLDPGIKWLEERTGIPVVGVIPYLQEVFPAEDSLDL
LERKTHKAHADLQITVVRLPRIANFTDFDPLESEPTVAVKYISPKQDLGHPDAVILPGTKTTIADLILLQKTGMAEAIQN
YAASGGTVLGICGGYQILGQMIADPEGIEGQAGRYQGLNLLPIRTVITGQKIARQRQVSSNFPQQGLPVNGFEIHQGRSR
VEPQGDSQAFQPLFDDVNLGLVDSCQSVWGSYLHGLFDNGPWRRAWLNRLRQQRGLKSLPTGVANYREQREQMLDNIATE
VENHLDLTPFLP

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_NOSS1 (Q8YUG9)

Other databases:

- EMBL:   BA000019
- PIR:   AB2103
- RefSeq:   NP_486417.1
- ProteinModelPortal:   Q8YUG9
- STRING:   Q8YUG9
- GeneID:   1105974
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr2377
- NMPDR:   fig|103690.1.peg.2684
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   AHTDLNP
- ProtClustDB:   PRK00784
- BioCyc:   NSP103690:ALR2377-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 54364; Mature: 54233

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 333-333 ACT_SITE 435-435

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMKSIMVVGTTSHAGKSLISTAICRILSRRGWRVAPFKGQNMALNAYVTANGGEIGYAQ
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECCCCCCHHH
AVQAWAAGVVPWVEMNPILLKPQGDMTSQVIIRGRPVGRVNAADYYEQYFEPGWRAIEES
HHHHHHHCCCCEEECCCEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHH
LQHLATEFDLVVCEGAGSPAEINLKHRDLTNMRVAKYLNAPTLLVVDIDRGGAFAHVVGT
HHHHHHHCCEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEEHH
LELLDPEERQLIKGVVINKFRGQRSLLDPGIKWLEERTGIPVVGVIPYLQEVFPAEDSLD
HHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCHH
LLERKTHKAHADLQITVVRLPRIANFTDFDPLESEPTVAVKYISPKQDLGHPDAVILPGT
HHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCEEEECCC
KTTIADLILLQKTGMAEAIQNYAASGGTVLGICGGYQILGQMIADPEGIEGQAGRYQGLN
HHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCE
LLPIRTVITGQKIARQRQVSSNFPQQGLPVNGFEIHQGRSRVEPQGDSQAFQPLFDDVNL
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCC
GLVDSCQSVWGSYLHGLFDNGPWRRAWLNRLRQQRGLKSLPTGVANYREQREQMLDNIAT
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHH
EVENHLDLTPFLP
HHHHCCCCCCCCC
>Mature Secondary Structure 
AMKSIMVVGTTSHAGKSLISTAICRILSRRGWRVAPFKGQNMALNAYVTANGGEIGYAQ
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECCCCCCHHH
AVQAWAAGVVPWVEMNPILLKPQGDMTSQVIIRGRPVGRVNAADYYEQYFEPGWRAIEES
HHHHHHHCCCCEEECCCEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHH
LQHLATEFDLVVCEGAGSPAEINLKHRDLTNMRVAKYLNAPTLLVVDIDRGGAFAHVVGT
HHHHHHHCCEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEEHH
LELLDPEERQLIKGVVINKFRGQRSLLDPGIKWLEERTGIPVVGVIPYLQEVFPAEDSLD
HHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCHH
LLERKTHKAHADLQITVVRLPRIANFTDFDPLESEPTVAVKYISPKQDLGHPDAVILPGT
HHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCEEEECCC
KTTIADLILLQKTGMAEAIQNYAASGGTVLGICGGYQILGQMIADPEGIEGQAGRYQGLN
HHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCE
LLPIRTVITGQKIARQRQVSSNFPQQGLPVNGFEIHQGRSRVEPQGDSQAFQPLFDDVNL
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCC
GLVDSCQSVWGSYLHGLFDNGPWRRAWLNRLRQQRGLKSLPTGVANYREQREQMLDNIAT
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHH
EVENHLDLTPFLP
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840