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The map label for this gene is gutB [H]

Identifier: 17229827

GI number: 17229827

Start: 2815238

End: 2816296

Strand: Direct

Name: gutB [H]

Synonym: alr2335

Alternate gene names: 17229827

Gene position: 2815238-2816296 (Clockwise)

Preceding gene: 17229823

Following gene: 17229828

Centisome position: 43.89

GC content: 44.85

Gene sequence:

>1059_bases
GTGAAAGCACAGGTATTTCGAGGTGTAAATCAACTCTCTTATGAAGAGATCCCCCTACCGATTCTGGAACCAGATGAAGT
GCTGGTACAGGTGCAAGTAGTCGGTTTGTGTCAGTCAGATATTAAAAAAATTCGTTATCCACTTTATGAACCACCACGCA
TCTTTGGTCATGAAACAGCCGGGACTATAGCCGCAGTGGGTTCTCAGGTACAGGGTTGGCAAGTAGGACAACGGGTGGCG
GTGATGCACCACATTCCTTGTATGCGCTGTGCCTATTGTATGAATGAGAATTTTTCCATGTGTGATGTTTATAAAAACAT
CTCCACCACTGCCGGATTTAATGCCAGTGGCGGTGGCTTTGCGGAGTATGTCAAGGTTCCGGGTCATATTGTAGAGAATG
GTGGCTTAATTCCGATTCCTGACGGGATCAGTTTTGAAGAAGCGAGTTTTGTTGAACCTACAAACTGTTGCTTAAAAGCA
GTCAAAAAAGCCCAGATTGCCCCAGGACAGACTGTTCTGGTAACTGGTGCAGGGCCGATTGGCTTAATGTTTGTCATGTT
GGTGAAATATTTCGGGGCAAAAGCGATCGCCACCGATTTGCTACCATCAAGAATAGAAAAAGCCCTCAATGTGGGTGCAG
AAGCCGCATTTGATGCCCGTGATCCTGATTTACCAGCCAAAGTTCATGCCCTCACCAATGGACTAGGGGTTGATGTTACT
TTGCTAGCTGTCCCCAGTGAAAAAGCCTTCTTTCAAGCTCTAGAATGTACGAGAAAAGGCGGCAAAATCTTGTTTTTCGC
CGAATTTCCAGACGAATTAGAAATTCCCATTAACCCAAATATTCTTTATCGTCGAGAAATCGACCTAATGGGCAGTTACA
GTTCATCCTATCGCCTCCAAAATCTCTCAGCTGATATCGTCTTTAATCGCCGCATCGATGTGCAAGCATTAATTAGCGAT
CGCTATCCCCTAAAAGATTTATCAGCCGCAGTAGATCAAGCGATCGCACCCACACCAGAAACTTACAAAATTCTGATTTA
TCCGAAAATTGGGGATTAG

Upstream 100 bases:

>100_bases
GATTTTGTGCGTAATGTTTTGTCTGATAAATCTGAAACCTTATAGCTGAACGTGGACGACTAATGTTAAATCTAGTAACG
TTGAGTAGTGGAAAACCAAC

Downstream 100 bases:

>100_bases
GGACTGGGAACTGGGTATTTTTACCTCTGTCCCATGTCTGTGTTGCTCTATCTCTTGCCCCTTTGTCAATATGGAAATAC
CAATTACATTACTTTTAGTT

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKAQVFRGVNQLSYEEIPLPILEPDEVLVQVQVVGLCQSDIKKIRYPLYEPPRIFGHETAGTIAAVGSQVQGWQVGQRVA
VMHHIPCMRCAYCMNENFSMCDVYKNISTTAGFNASGGGFAEYVKVPGHIVENGGLIPIPDGISFEEASFVEPTNCCLKA
VKKAQIAPGQTVLVTGAGPIGLMFVMLVKYFGAKAIATDLLPSRIEKALNVGAEAAFDARDPDLPAKVHALTNGLGVDVT
LLAVPSEKAFFQALECTRKGGKILFFAEFPDELEIPINPNILYRREIDLMGSYSSSYRLQNLSADIVFNRRIDVQALISD
RYPLKDLSAAVDQAIAPTPETYKILIYPKIGD

Sequences:

>Translated_352_residues
MKAQVFRGVNQLSYEEIPLPILEPDEVLVQVQVVGLCQSDIKKIRYPLYEPPRIFGHETAGTIAAVGSQVQGWQVGQRVA
VMHHIPCMRCAYCMNENFSMCDVYKNISTTAGFNASGGGFAEYVKVPGHIVENGGLIPIPDGISFEEASFVEPTNCCLKA
VKKAQIAPGQTVLVTGAGPIGLMFVMLVKYFGAKAIATDLLPSRIEKALNVGAEAAFDARDPDLPAKVHALTNGLGVDVT
LLAVPSEKAFFQALECTRKGGKILFFAEFPDELEIPINPNILYRREIDLMGSYSSSYRLQNLSADIVFNRRIDVQALISD
RYPLKDLSAAVDQAIAPTPETYKILIYPKIGD
>Mature_352_residues
MKAQVFRGVNQLSYEEIPLPILEPDEVLVQVQVVGLCQSDIKKIRYPLYEPPRIFGHETAGTIAAVGSQVQGWQVGQRVA
VMHHIPCMRCAYCMNENFSMCDVYKNISTTAGFNASGGGFAEYVKVPGHIVENGGLIPIPDGISFEEASFVEPTNCCLKA
VKKAQIAPGQTVLVTGAGPIGLMFVMLVKYFGAKAIATDLLPSRIEKALNVGAEAAFDARDPDLPAKVHALTNGLGVDVT
LLAVPSEKAFFQALECTRKGGKILFFAEFPDELEIPINPNILYRREIDLMGSYSSSYRLQNLSADIVFNRRIDVQALISD
RYPLKDLSAAVDQAIAPTPETYKILIYPKIGD

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=336, Percent_Identity=26.7857142857143, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4501933, Length=345, Percent_Identity=26.0869565217391, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI71565152, Length=355, Percent_Identity=25.3521126760563, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI156523966, Length=347, Percent_Identity=23.6311239193084, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4501939, Length=347, Percent_Identity=23.6311239193084, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1788073, Length=353, Percent_Identity=28.0453257790368, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI226510992, Length=338, Percent_Identity=28.4023668639053, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788075, Length=335, Percent_Identity=27.7611940298507, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790045, Length=331, Percent_Identity=25.6797583081571, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082125, Length=365, Percent_Identity=26.5753424657534, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788407, Length=349, Percent_Identity=26.647564469914, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1787863, Length=226, Percent_Identity=35.3982300884956, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1790718, Length=311, Percent_Identity=26.6881028938907, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786825, Length=308, Percent_Identity=27.5974025974026, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081918, Length=332, Percent_Identity=26.8072289156627, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082401, Length=339, Percent_Identity=27.1386430678466, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=339, Percent_Identity=28.3185840707965, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17562878, Length=335, Percent_Identity=25.9701492537313, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17562584, Length=295, Percent_Identity=29.1525423728814, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71988145, Length=312, Percent_Identity=26.6025641025641, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=308, Percent_Identity=28.8961038961039, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI25146526, Length=360, Percent_Identity=25.2777777777778, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323099, Length=344, Percent_Identity=27.906976744186, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319955, Length=358, Percent_Identity=25.6983240223464, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322619, Length=358, Percent_Identity=25.6983240223464, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319258, Length=225, Percent_Identity=33.7777777777778, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=303, Percent_Identity=27.3927392739274, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319257, Length=366, Percent_Identity=24.8633879781421, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319621, Length=275, Percent_Identity=28.7272727272727, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=266, Percent_Identity=27.0676691729323, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=298, Percent_Identity=24.496644295302, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319520, Length=261, Percent_Identity=25.6704980842912, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320033, Length=352, Percent_Identity=23.8636363636364, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17137530, Length=354, Percent_Identity=26.8361581920904, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17737897, Length=357, Percent_Identity=27.4509803921569, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 38428; Mature: 38428

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAQVFRGVNQLSYEEIPLPILEPDEVLVQVQVVGLCQSDIKKIRYPLYEPPRIFGHETA
CCCHHHHCHHHCCHHHCCCCCCCCCCEEEEEEEEHHCHHHHHHHCCCCCCCCHHCCCCCC
GTIAAVGSQVQGWQVGQRVAVMHHIPCMRCAYCMNENFSMCDVYKNISTTAGFNASGGGF
CHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCH
AEYVKVPGHIVENGGLIPIPDGISFEEASFVEPTNCCLKAVKKAQIAPGQTVLVTGAGPI
HHHEECCCEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHH
GLMFVMLVKYFGAKAIATDLLPSRIEKALNVGAEAAFDARDPDLPAKVHALTNGLGVDVT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEE
LLAVPSEKAFFQALECTRKGGKILFFAEFPDELEIPINPNILYRREIDLMGSYSSSYRLQ
EEEECCHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCEEEEEEEEEECCCCCCEEEE
NLSADIVFNRRIDVQALISDRYPLKDLSAAVDQAIAPTPETYKILIYPKIGD
CCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MKAQVFRGVNQLSYEEIPLPILEPDEVLVQVQVVGLCQSDIKKIRYPLYEPPRIFGHETA
CCCHHHHCHHHCCHHHCCCCCCCCCCEEEEEEEEHHCHHHHHHHCCCCCCCCHHCCCCCC
GTIAAVGSQVQGWQVGQRVAVMHHIPCMRCAYCMNENFSMCDVYKNISTTAGFNASGGGF
CHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCH
AEYVKVPGHIVENGGLIPIPDGISFEEASFVEPTNCCLKAVKKAQIAPGQTVLVTGAGPI
HHHEECCCEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHH
GLMFVMLVKYFGAKAIATDLLPSRIEKALNVGAEAAFDARDPDLPAKVHALTNGLGVDVT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEE
LLAVPSEKAFFQALECTRKGGKILFFAEFPDELEIPINPNILYRREIDLMGSYSSSYRLQ
EEEECCHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCEEEEEEEEEECCCCCCEEEE
NLSADIVFNRRIDVQALISDRYPLKDLSAAVDQAIAPTPETYKILIYPKIGD
CCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]