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The map label for this gene is gloA [H]

Identifier: 17229813

GI number: 17229813

Start: 2795099

End: 2795536

Strand: Direct

Name: gloA [H]

Synonym: alr2321

Alternate gene names: 17229813

Gene position: 2795099-2795536 (Clockwise)

Preceding gene: 17229805

Following gene: 17229814

Centisome position: 43.58

GC content: 43.38

Gene sequence:

>438_bases
ATGCGTTTACTACATACAATGCTACGGGTGGGTAACCTGGAAGAGTCTTTGAAATTCTACTGTGATGTCCTGGGCATGAA
GTTACTACGGAAAAAGGATTACCCAGGAGGAGAATTTACCCTAGCTTTTATTGGCTACGGCGATGAGAGTGATAACACAG
TTATAGAACTAACCTACAATTGGGGAGTAGAGAAGTACGAATTAGGTAATGCTTACGGTCATATTGCTCTTGGTGTAGAT
GATATTTATGCCACCTGTGAGTCTATTAAAACTCAGGGCGGTAAGGTGGTTAGGGAACCAGGGCCAATGAAGCATGGCTC
GACTGTGATTGCTTTCGTCGAAGACCCAGATGGATACAAGATAGAACTAATCCAGTTGAGTAATCAAGGCGCGACAGTGA
AACAAGATTCATCAGAACAGCTAGTGAAACAGGGTTAG

Upstream 100 bases:

>100_bases
CTGCCCTCTGCTCCCTTGCCTCTTATGCCCCCAATCTCCAATCCCTCTGTACAATGTTGGCAGAAATCAATATTTTACAG
AAAACAACCTTAAGGTGATT

Downstream 100 bases:

>100_bases
AGAATGGAGGTTGATGAGTAATGAGTGGGGAGTTAATAAAATTACTGCTGATTTTTTCCCTACTCACTCATCCTGAAAGG
TGTAGGTTTTTTACACTACA

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MRLLHTMLRVGNLEESLKFYCDVLGMKLLRKKDYPGGEFTLAFIGYGDESDNTVIELTYNWGVEKYELGNAYGHIALGVD
DIYATCESIKTQGGKVVREPGPMKHGSTVIAFVEDPDGYKIELIQLSNQGATVKQDSSEQLVKQG

Sequences:

>Translated_145_residues
MRLLHTMLRVGNLEESLKFYCDVLGMKLLRKKDYPGGEFTLAFIGYGDESDNTVIELTYNWGVEKYELGNAYGHIALGVD
DIYATCESIKTQGGKVVREPGPMKHGSTVIAFVEDPDGYKIELIQLSNQGATVKQDSSEQLVKQG
>Mature_145_residues
MRLLHTMLRVGNLEESLKFYCDVLGMKLLRKKDYPGGEFTLAFIGYGDESDNTVIELTYNWGVEKYELGNAYGHIALGVD
DIYATCESIKTQGGKVVREPGPMKHGSTVIAFVEDPDGYKIELIQLSNQGATVKQDSSEQLVKQG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=157, Percent_Identity=38.2165605095541, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI217330598, Length=137, Percent_Identity=31.3868613138686, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787940, Length=130, Percent_Identity=65.3846153846154, Blast_Score=180, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=41.726618705036, Blast_Score=100, Evalue=9e-23,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=38.3561643835616, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920404, Length=134, Percent_Identity=29.8507462686567, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16097; Mature: 16097

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGNLEESLKFYCDVLGMKLLRKKDYPGGEFTLAFIGYGDESDNTVIELTYN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WGVEKYELGNAYGHIALGVDDIYATCESIKTQGGKVVREPGPMKHGSTVIAFVEDPDGYK
CCCEEEECCCCEEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCE
IELIQLSNQGATVKQDSSEQLVKQG
EEEEEECCCCCEEECCCHHHHHHCC
>Mature Secondary Structure
MRLLHTMLRVGNLEESLKFYCDVLGMKLLRKKDYPGGEFTLAFIGYGDESDNTVIELTYN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WGVEKYELGNAYGHIALGVDDIYATCESIKTQGGKVVREPGPMKHGSTVIAFVEDPDGYK
CCCEEEECCCCEEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCE
IELIQLSNQGATVKQDSSEQLVKQG
EEEEEECCCCCEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]