Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 17229209

GI number: 17229209

Start: 2066137

End: 2068170

Strand: Reverse

Name: ligA

Synonym: all1717

Alternate gene names: 17229209

Gene position: 2068170-2066137 (Counterclockwise)

Preceding gene: 17229211

Following gene: 17229208

Centisome position: 32.25

GC content: 44.25

Gene sequence:

>2034_bases
ATGATACAAACTCACTCTGAAGTCAAGCGCGTAGAGGAACTGCGCCGGTTGTTGCAACAAGCTAGCTATGCTTATTATGT
CTCGGATGCACCTATCATGGAGGATGCAGTTTATGACCAACTGTATCGGGAACTGCAACAACTAGAAATTCAGCATCCAG
AATTAGTTACACCAGATAGTCCGACACAGCGCATCGGGGAAAGGCCAGCAACACAGTTTCTCTCGGTACGTCACAATATT
CCCTTATACAGTTTAGAGAATGCCTTTAATGTAGAGGAGTTGCAAGCCTGGGATCAACGTTGGCGGCGACAAGTTGCGCC
AACGGAGGCGGAATATGTGGCTGAACTGAAAATTGATGGTTCGGCGATCGCTCTCACTTACCAAAATGGTATTTTAGTTA
GGGGTGCAACTAGGGGTGATGGGGTGACGGGGGAAGATATCACCCAAAATGTCCGAACTATTCGCTCCATTCCCTTGCGG
TTGAATTTTGATGGTATAGAAAAAGTTGAAAAGGTAGAAGTCAGGGGTGAGGCTTTTTTACCTTTGGAAGTGTTTAAACA
AATCAATGAAGAAAGACAAAAAGCCGGGGAACAGTTATTTGCTAATCCGCGTAATGCGGCGGCTGGTACACTCAGACAGC
TAGACTCCAGAATTGTGGCACGACGGCGGTTAGACTTTTTCGCCTATACCCTGCATATTTCGGGGATGGATGACGCGAGT
ATTGCTAATACCCAATGGGAAGCGTTGGAATTGTTGCAAAAAATGGGTTTTCGGGTGAATCCTAACCACAAGCTTTGTCA
ATCTTTAGCTGAGGTGGCAGATTATTATAAATACTGGGATACAGAAAGATTAAATTTACCCTACATGACCGATGGGGTAG
TATTAAAGCTCAATTCCTTTAAGCTCCAAGAACAGTTAGGTTTTACCCAAAGATTTCCCCGGTGGGCTGTGGCGTTGAAG
TATCCCGCCGAAGAAGCACCCACCCGTGTAGAAAATATTGCGGTGAATGTGGGGAGGACGGGGGCGTTAACACCATTGGC
TCAAATGCGTCCAGTGCAGTTGGCGGGAACTACTGTTTCTAGAGCTACTTTACATAATAGCGATCGCATCGCGCAATTAG
ACATCCGCATCGGTGATACTGTCATTGTCCGTAAGGCGGGGGAAATCATCCCCGAAGTTGTGAGGGTAATTAAGGAACTG
CGCCCGGCTGACACCCAACCCTTTATTATGCCTACTCATTGTCCTGTTTGTGGACAGCCTGTGGTGCGAGAGACGGGCGA
GGCGGTGACTCGCTGTGTTAATGCTTCTTGTCCAGCAATTCTCAAAGGCTCAATTGAACACTGGGTGAGTCGTGATGCTT
TAGATATTAAAGGTGTGGGGGAAAAACTTGTATATCAGCTTGTAGATAAAGGCTTGGTGCATTCCGTCGCTGATTTATAT
GACTTGACAACAGAGCGATTATTTGCATTAGAAAGAATGGGAGAAAAATCGGCACAGAAATTAATTGAGGCGATCGCTCA
ATCAAAAAATCAGCCTTGGTCAAGGGTATTATATGGTTTGGGCATCCGTCATGTGGGTAGTGTTAATGCCCAATTATTGA
GCGAAAAATACCGCGCAGTCGCAGAATTATCTTCAGCCAAACAATCAGATATAGAAGGTATTTATGGGATTGGTGCAGAA
ATTGCCCAGTCTGTTTACCAATGGTTTCGCATTAATGCCAATCAAAGTTTAATTGAACGCTTACAAGCGGCAGGGTTAAA
ACTTGCGAACACAGAAGAAATACCAGTCATGAATAATGGTATCCTAAAACTAAATGGTAAAACCTTTGTAGTCACAGGTA
CCTTGCCAACCTTAAAACGGGATGAAGTTAAAGCTTTAATTCAAAAAGCTGGGGGTAAAGTGACTGATTCCGTAAGTAAA
AAAACCGATTATTTGGTTGTAGGTGAAGATGCAGGCTCCAAATTAGAAAAAGCACAAGCATTGGGGATTGCCCAGTTAAC
TGAAACACAGTTGTTAGAAATTTTAGAAGAATAA

Upstream 100 bases:

>100_bases
GAACCACAGACTATTAATCTAGGGTTTAGTATCACAAGCCTGCTACTGGCAGGTTTTATCGTCTTTATATTTTATCCGCT
AGGTATTTTGCAAAAGTCTA

Downstream 100 bases:

>100_bases
AGACTAAGGATATTTTAAGCATTGAACTCTAGATATCGGGTCTACATCAACCAAATAAACTAAATTAAAATTTTTCCTCT
TCCTAAGAGGATGTTTTAAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MIQTHSEVKRVEELRRLLQQASYAYYVSDAPIMEDAVYDQLYRELQQLEIQHPELVTPDSPTQRIGERPATQFLSVRHNI
PLYSLENAFNVEELQAWDQRWRRQVAPTEAEYVAELKIDGSAIALTYQNGILVRGATRGDGVTGEDITQNVRTIRSIPLR
LNFDGIEKVEKVEVRGEAFLPLEVFKQINEERQKAGEQLFANPRNAAAGTLRQLDSRIVARRRLDFFAYTLHISGMDDAS
IANTQWEALELLQKMGFRVNPNHKLCQSLAEVADYYKYWDTERLNLPYMTDGVVLKLNSFKLQEQLGFTQRFPRWAVALK
YPAEEAPTRVENIAVNVGRTGALTPLAQMRPVQLAGTTVSRATLHNSDRIAQLDIRIGDTVIVRKAGEIIPEVVRVIKEL
RPADTQPFIMPTHCPVCGQPVVRETGEAVTRCVNASCPAILKGSIEHWVSRDALDIKGVGEKLVYQLVDKGLVHSVADLY
DLTTERLFALERMGEKSAQKLIEAIAQSKNQPWSRVLYGLGIRHVGSVNAQLLSEKYRAVAELSSAKQSDIEGIYGIGAE
IAQSVYQWFRINANQSLIERLQAAGLKLANTEEIPVMNNGILKLNGKTFVVTGTLPTLKRDEVKALIQKAGGKVTDSVSK
KTDYLVVGEDAGSKLEKAQALGIAQLTETQLLEILEE

Sequences:

>Translated_677_residues
MIQTHSEVKRVEELRRLLQQASYAYYVSDAPIMEDAVYDQLYRELQQLEIQHPELVTPDSPTQRIGERPATQFLSVRHNI
PLYSLENAFNVEELQAWDQRWRRQVAPTEAEYVAELKIDGSAIALTYQNGILVRGATRGDGVTGEDITQNVRTIRSIPLR
LNFDGIEKVEKVEVRGEAFLPLEVFKQINEERQKAGEQLFANPRNAAAGTLRQLDSRIVARRRLDFFAYTLHISGMDDAS
IANTQWEALELLQKMGFRVNPNHKLCQSLAEVADYYKYWDTERLNLPYMTDGVVLKLNSFKLQEQLGFTQRFPRWAVALK
YPAEEAPTRVENIAVNVGRTGALTPLAQMRPVQLAGTTVSRATLHNSDRIAQLDIRIGDTVIVRKAGEIIPEVVRVIKEL
RPADTQPFIMPTHCPVCGQPVVRETGEAVTRCVNASCPAILKGSIEHWVSRDALDIKGVGEKLVYQLVDKGLVHSVADLY
DLTTERLFALERMGEKSAQKLIEAIAQSKNQPWSRVLYGLGIRHVGSVNAQLLSEKYRAVAELSSAKQSDIEGIYGIGAE
IAQSVYQWFRINANQSLIERLQAAGLKLANTEEIPVMNNGILKLNGKTFVVTGTLPTLKRDEVKALIQKAGGKVTDSVSK
KTDYLVVGEDAGSKLEKAQALGIAQLTETQLLEILEE
>Mature_677_residues
MIQTHSEVKRVEELRRLLQQASYAYYVSDAPIMEDAVYDQLYRELQQLEIQHPELVTPDSPTQRIGERPATQFLSVRHNI
PLYSLENAFNVEELQAWDQRWRRQVAPTEAEYVAELKIDGSAIALTYQNGILVRGATRGDGVTGEDITQNVRTIRSIPLR
LNFDGIEKVEKVEVRGEAFLPLEVFKQINEERQKAGEQLFANPRNAAAGTLRQLDSRIVARRRLDFFAYTLHISGMDDAS
IANTQWEALELLQKMGFRVNPNHKLCQSLAEVADYYKYWDTERLNLPYMTDGVVLKLNSFKLQEQLGFTQRFPRWAVALK
YPAEEAPTRVENIAVNVGRTGALTPLAQMRPVQLAGTTVSRATLHNSDRIAQLDIRIGDTVIVRKAGEIIPEVVRVIKEL
RPADTQPFIMPTHCPVCGQPVVRETGEAVTRCVNASCPAILKGSIEHWVSRDALDIKGVGEKLVYQLVDKGLVHSVADLY
DLTTERLFALERMGEKSAQKLIEAIAQSKNQPWSRVLYGLGIRHVGSVNAQLLSEKYRAVAELSSAKQSDIEGIYGIGAE
IAQSVYQWFRINANQSLIERLQAAGLKLANTEEIPVMNNGILKLNGKTFVVTGTLPTLKRDEVKALIQKAGGKVTDSVSK
KTDYLVVGEDAGSKLEKAQALGIAQLTETQLLEILEE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=125, Percent_Identity=40, Blast_Score=78, Evalue=4e-14,
Organism=Escherichia coli, GI1788750, Length=674, Percent_Identity=45.9940652818991, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI87082305, Length=575, Percent_Identity=23.8260869565217, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_NOSS1 (Q8YW98)

Other databases:

- EMBL:   BA000019
- PIR:   AG2020
- RefSeq:   NP_485757.1
- HSSP:   P26996
- ProteinModelPortal:   Q8YW98
- STRING:   Q8YW98
- GeneID:   1105309
- GenomeReviews:   BA000019_GR
- KEGG:   ana:all1717
- NMPDR:   fig|103690.1.peg.2024
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q8YW98
- ProtClustDB:   PRK07956
- BioCyc:   NSP103690:ALL1717-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75793; Mature: 75793

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 117-117 BINDING 115-115 BINDING 138-138 BINDING 177-177 BINDING 296-296 BINDING 320-320

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTHSEVKRVEELRRLLQQASYAYYVSDAPIMEDAVYDQLYRELQQLEIQHPELVTPDS
CCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PTQRIGERPATQFLSVRHNIPLYSLENAFNVEELQAWDQRWRRQVAPTEAEYVAELKIDG
HHHHHCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHEEEEEECC
SAIALTYQNGILVRGATRGDGVTGEDITQNVRTIRSIPLRLNFDGIEKVEKVEVRGEAFL
CEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCEEC
PLEVFKQINEERQKAGEQLFANPRNAAAGTLRQLDSRIVARRRLDFFAYTLHISGMDDAS
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
IANTQWEALELLQKMGFRVNPNHKLCQSLAEVADYYKYWDTERLNLPYMTDGVVLKLNSF
CCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCH
KLQEQLGFTQRFPRWAVALKYPAEEAPTRVENIAVNVGRTGALTPLAQMRPVQLAGTTVS
HHHHHCCHHHCCCCEEEEEECCCCCCCCHHHHEEEECCCCCCCCCHHHCCCEEEECCCHH
RATLHNSDRIAQLDIRIGDTVIVRKAGEIIPEVVRVIKELRPADTQPFIMPTHCPVCGQP
HHHHCCCCCEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCH
VVRETGEAVTRCVNASCPAILKGSIEHWVSRDALDIKGVGEKLVYQLVDKGLVHSVADLY
HHHHHHHHHHHHHCCCCCHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DLTTERLFALERMGEKSAQKLIEAIAQSKNQPWSRVLYGLGIRHVGSVNAQLLSEKYRAV
HHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHCCHHHHHHHHHHHHH
AELSSAKQSDIEGIYGIGAEIAQSVYQWFRINANQSLIERLQAAGLKLANTEEIPVMNNG
HHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEECCCCCCCEECCC
ILKLNGKTFVVTGTLPTLKRDEVKALIQKAGGKVTDSVSKKTDYLVVGEDAGSKLEKAQA
EEEECCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCEEEEECCCCCHHHHHHH
LGIAQLTETQLLEILEE
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIQTHSEVKRVEELRRLLQQASYAYYVSDAPIMEDAVYDQLYRELQQLEIQHPELVTPDS
CCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PTQRIGERPATQFLSVRHNIPLYSLENAFNVEELQAWDQRWRRQVAPTEAEYVAELKIDG
HHHHHCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHEEEEEECC
SAIALTYQNGILVRGATRGDGVTGEDITQNVRTIRSIPLRLNFDGIEKVEKVEVRGEAFL
CEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCEEC
PLEVFKQINEERQKAGEQLFANPRNAAAGTLRQLDSRIVARRRLDFFAYTLHISGMDDAS
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
IANTQWEALELLQKMGFRVNPNHKLCQSLAEVADYYKYWDTERLNLPYMTDGVVLKLNSF
CCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCH
KLQEQLGFTQRFPRWAVALKYPAEEAPTRVENIAVNVGRTGALTPLAQMRPVQLAGTTVS
HHHHHCCHHHCCCCEEEEEECCCCCCCCHHHHEEEECCCCCCCCCHHHCCCEEEECCCHH
RATLHNSDRIAQLDIRIGDTVIVRKAGEIIPEVVRVIKELRPADTQPFIMPTHCPVCGQP
HHHHCCCCCEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCH
VVRETGEAVTRCVNASCPAILKGSIEHWVSRDALDIKGVGEKLVYQLVDKGLVHSVADLY
HHHHHHHHHHHHHCCCCCHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DLTTERLFALERMGEKSAQKLIEAIAQSKNQPWSRVLYGLGIRHVGSVNAQLLSEKYRAV
HHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHCCHHHHHHHHHHHHH
AELSSAKQSDIEGIYGIGAEIAQSVYQWFRINANQSLIERLQAAGLKLANTEEIPVMNNG
HHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEECCCCCCCEECCC
ILKLNGKTFVVTGTLPTLKRDEVKALIQKAGGKVTDSVSKKTDYLVVGEDAGSKLEKAQA
EEEECCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCEEEEECCCCCHHHHHHH
LGIAQLTETQLLEILEE
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840