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The map label for this gene is purU [H]

Identifier: 17229115

GI number: 17229115

Start: 1911264

End: 1912118

Strand: Direct

Name: purU [H]

Synonym: alr1623

Alternate gene names: 17229115

Gene position: 1911264-1912118 (Clockwise)

Preceding gene: 17229114

Following gene: 17229116

Centisome position: 29.8

GC content: 41.52

Gene sequence:

>855_bases
GTGACCAACCCGACTGCAACTCTACTAATTTCATGTCCCGACCAACGGGGATTAGTCGCTAAAATCGCTAACTTTATCTA
CGCTAATGGTGGGAATATTATTCACGCTGACCAACATACCGATTTTGCGGCTGGGTTATTCCTCACGCGCATTGAATGGC
AGTTAGAGGGTTTCAATTTGCCGCGAGAGATTATTGGGGCCGCGTTTAATGCCATCGCTCAACCTCTAGGCGCTAAGTGG
GAACTACATTTTTCTGATACAATCCCACGTATTGCCATTTGGGTAAGTCGCCAAGACCATTGCCTTTATGATTTAATTTG
GCGACAACGCGCCAAAGAAATTGCGGTTGAGATTCCTTTAATTATTAGTAACCATCCCCATTTAAAAGTAGTTGCAGATC
AATTTGGCATCGACTTTCGCCACATTCCTATTAATAAAGATAACAAAGCGGAACAGGAAGCCCAACAATTAGAATTACTG
CAACAATATGAAATTGATTTAGTTGTATTAGCAAAATATATGCAAATTGTTAGTGCAGACTTTATTACGAAATTTCCGCA
AATTATTAATATTCATCACTCATTCTTACCAGCATTCGTAGGCGCAAACCCCTACCATAGAGCCTTTGAACGCGGCGTGA
AAGTTATTGGTGCCACAGCTCACTATGCTACTCCGGAATTAGATGCTGGGCCAATTATTGAACAAGATGTAGTCAGAGTT
AGCCACCGCGATGAAGTTGAGGATTTAATTAGAAAGGGTAAGGATTTAGAAAGAGTCGTTTTAGCCAGAGCCGTGCGCCT
ACATCTGCAAAATCGGGTTTTAGTTTATGGAAATCGCACTGTAGTTTTTGAATGA

Upstream 100 bases:

>100_bases
CCTTAACCAACTCCCACCAATAGCCAATAAGCAGATATACAGATAAAATTCAGAATTCACCCTTAGGGTATTTTGAATTT
CAAATTTGGAGCGAACTGAC

Downstream 100 bases:

>100_bases
AAGTTGGGTGTAAAGACTAATCAATTCAAAATTCCAAAGCACATCTTTTCATGGTGGCTGTTGACAGTTGACAGTTAACC
GTCAACTGTCAACTGTCAAC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTNPTATLLISCPDQRGLVAKIANFIYANGGNIIHADQHTDFAAGLFLTRIEWQLEGFNLPREIIGAAFNAIAQPLGAKW
ELHFSDTIPRIAIWVSRQDHCLYDLIWRQRAKEIAVEIPLIISNHPHLKVVADQFGIDFRHIPINKDNKAEQEAQQLELL
QQYEIDLVVLAKYMQIVSADFITKFPQIINIHHSFLPAFVGANPYHRAFERGVKVIGATAHYATPELDAGPIIEQDVVRV
SHRDEVEDLIRKGKDLERVVLARAVRLHLQNRVLVYGNRTVVFE

Sequences:

>Translated_284_residues
MTNPTATLLISCPDQRGLVAKIANFIYANGGNIIHADQHTDFAAGLFLTRIEWQLEGFNLPREIIGAAFNAIAQPLGAKW
ELHFSDTIPRIAIWVSRQDHCLYDLIWRQRAKEIAVEIPLIISNHPHLKVVADQFGIDFRHIPINKDNKAEQEAQQLELL
QQYEIDLVVLAKYMQIVSADFITKFPQIINIHHSFLPAFVGANPYHRAFERGVKVIGATAHYATPELDAGPIIEQDVVRV
SHRDEVEDLIRKGKDLERVVLARAVRLHLQNRVLVYGNRTVVFE
>Mature_283_residues
TNPTATLLISCPDQRGLVAKIANFIYANGGNIIHADQHTDFAAGLFLTRIEWQLEGFNLPREIIGAAFNAIAQPLGAKWE
LHFSDTIPRIAIWVSRQDHCLYDLIWRQRAKEIAVEIPLIISNHPHLKVVADQFGIDFRHIPINKDNKAEQEAQQLELLQ
QYEIDLVVLAKYMQIVSADFITKFPQIINIHHSFLPAFVGANPYHRAFERGVKVIGATAHYATPELDAGPIIEQDVVRVS
HRDEVEDLIRKGKDLERVVLARAVRLHLQNRVLVYGNRTVVFE

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=206, Percent_Identity=31.0679611650485, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869995, Length=206, Percent_Identity=31.0679611650485, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869993, Length=206, Percent_Identity=31.0679611650485, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787483, Length=273, Percent_Identity=42.8571428571429, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1788846, Length=132, Percent_Identity=31.0606060606061, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=166, Percent_Identity=33.7349397590361, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24582400, Length=206, Percent_Identity=28.6407766990291, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32270; Mature: 32138

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPTATLLISCPDQRGLVAKIANFIYANGGNIIHADQHTDFAAGLFLTRIEWQLEGFNL
CCCCCEEEEEECCCCCCHHHHHHHHHEECCCCEEECCCCCCHHHHHHEEEEEEEECCCCC
PREIIGAAFNAIAQPLGAKWELHFSDTIPRIAIWVSRQDHCLYDLIWRQRAKEIAVEIPL
CHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEEE
IISNHPHLKVVADQFGIDFRHIPINKDNKAEQEAQQLELLQQYEIDLVVLAKYMQIVSAD
EECCCCCEEEEEHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FITKFPQIINIHHSFLPAFVGANPYHRAFERGVKVIGATAHYATPELDAGPIIEQDVVRV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHH
SHRDEVEDLIRKGKDLERVVLARAVRLHLQNRVLVYGNRTVVFE
HCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECEEEEEC
>Mature Secondary Structure 
TNPTATLLISCPDQRGLVAKIANFIYANGGNIIHADQHTDFAAGLFLTRIEWQLEGFNL
CCCCEEEEEECCCCCCHHHHHHHHHEECCCCEEECCCCCCHHHHHHEEEEEEEECCCCC
PREIIGAAFNAIAQPLGAKWELHFSDTIPRIAIWVSRQDHCLYDLIWRQRAKEIAVEIPL
CHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEEE
IISNHPHLKVVADQFGIDFRHIPINKDNKAEQEAQQLELLQQYEIDLVVLAKYMQIVSAD
EECCCCCEEEEEHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FITKFPQIINIHHSFLPAFVGANPYHRAFERGVKVIGATAHYATPELDAGPIIEQDVVRV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHH
SHRDEVEDLIRKGKDLERVVLARAVRLHLQNRVLVYGNRTVVFE
HCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]