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The map label for this gene is nirA [H]

Identifier: 17228103

GI number: 17228103

Start: 704679

End: 706289

Strand: Direct

Name: nirA [H]

Synonym: alr0607

Alternate gene names: 17228103

Gene position: 704679-706289 (Clockwise)

Preceding gene: 17228096

Following gene: 17228104

Centisome position: 10.99

GC content: 45.62

Gene sequence:

>1611_bases
ATGACAGATACAGTAACTACCCCCAAAGCCAGCCTCAATAAGTTTGAGAAATTCAAAGCCGAAAAAGATGGACTTGCCAT
CAAGTCAGAGATCGAAAAAATTGCCTCTTTGGGATGGGAAGCAATGGACGCAACAGACCGAGATCATCGCCTCAAATGGG
TGGGTGTATTCTTTCGCCCAGTCACCCCTGGTAAATTTATGATGCGGATGCGGATGCCGAATGGTATCCTCACCAGCGAT
CAGATGCGTGTTTTAGCCGAAGTGGTGCAGCGTTACGGAGATGACGGCAACGCTGATATTACAACTAGGCAGAATATTCA
ACTACGAGGTATCAGAATAGAAGACTTACCGCACATATTCAATAAATTTCATGCAGTAGGTTTAACCAGTGTGCAGTCAG
GGATGGACAACATCCGTAACATCACAGGCGACCCGATAGCGGGGTTAGATGCGGATGAGTTGTATGACACCCGTGAGTTA
GTGCAGCAAATTCAGGATATGCTCACCAACAAAGGAGAAGGCAATCGAGAGTTTAGTAATTTGCCTCGTAAATTTAATAT
TGCGATCGCCGGTGGACGGGATAATTCAGTTCATGCGGAAATCAACGATTTAGCCTTTGTTCCAGCATTTAAAGAAGGGA
TTGGAGATTGGGTATTGGGGAATGGGGAAGAATCATCTACTTACCAAAAAGTCTTTGGATTTAACGTGTTAGTTGGTGGT
TTCTTTTCTGCTAAACGCTGTGAGGCGGCGATTCCTTTGAATGCTTGGGTAACTCCGGAAGAAGTCTTACCCTTATGTAG
AGCAATTTTAGAGGTCTATCGTGACAATGGACTCAGGGCTAATCGGCTCAAGTCTCGCTTGATGTGGCTAATTGATGAAT
GGGGTATAGATAAGTTTCGGGCAGAAGTCGAACAGCGTTTGGGTAAATCCTTACTCCCCGCAGCCCCCAAAGACGAAATT
GATTGGGAAAAACGCGACCATATCGGAGTCTATAAGCAAAAGCAAGAGGGATTGAACTATGTAGGGTTACACATCCCTGT
AGGTAGATTGTATGCCGAGGATATGTTTGAATTGGCTCGGATAGCCGATGTATACGGTAGCGGTGAAATCCGCATGACTG
TTGAACAAAACATCATCATTCCCAACATTACCGACTCGCGGTTAAGGACTTTGTTGACAGATCCCTTACTAGAGAGATTT
TCTCTTGATCCTGGAGCATTGACGCGATCGCTAGTTTCCTGCACGGGCGCACAATTTTGCAACTTCGCCCTCATCGAAAC
CAAAAACCGCGCCCTAGAAATGATTAAAGGCTTAGAAGCAGAATTGACCTTTACTCGTCCAGTGCGAATCCATTGGACAG
GTTGCCCCAACTCCTGCGGACAGCCCCAAGTTGCAGACATTGGCTTAATGGGAACAAAAGCTCGTAAAAACGGTAAAGCC
GTGGAAGGTGTTGACATCTATATGGGTGGCAAAGTCGGCAAAGATGCACATTTAGGTAGCTGTGTACAAAAAGGCATCCC
CTGCGAAGACTTGCACCTAGTATTACGAGACTTACTCATTACTAATTTTGGAGCCAAACCCAGACAGGAAGCCTTAGTTA
CCAGCCAATAA

Upstream 100 bases:

>100_bases
TCTTTCAAGATTACTAAATTGCTATCACTAGACCTCGTAGAACTAGCAAAGACTACGGGTGGATTGATCTTGAGCAAAAA
AACTTTATGAGAACCAGCTC

Downstream 100 bases:

>100_bases
GGGAGCAGGGGAGAAAACAATTCAAAATTCAAAATTCAAAAAAAGAGTGGGGTAGTGAGCATGGGGAAGAAAATTGACTA
TTGACTGTTGACTAATGACA

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MTDTVTTPKASLNKFEKFKAEKDGLAIKSEIEKIASLGWEAMDATDRDHRLKWVGVFFRPVTPGKFMMRMRMPNGILTSD
QMRVLAEVVQRYGDDGNADITTRQNIQLRGIRIEDLPHIFNKFHAVGLTSVQSGMDNIRNITGDPIAGLDADELYDTREL
VQQIQDMLTNKGEGNREFSNLPRKFNIAIAGGRDNSVHAEINDLAFVPAFKEGIGDWVLGNGEESSTYQKVFGFNVLVGG
FFSAKRCEAAIPLNAWVTPEEVLPLCRAILEVYRDNGLRANRLKSRLMWLIDEWGIDKFRAEVEQRLGKSLLPAAPKDEI
DWEKRDHIGVYKQKQEGLNYVGLHIPVGRLYAEDMFELARIADVYGSGEIRMTVEQNIIIPNITDSRLRTLLTDPLLERF
SLDPGALTRSLVSCTGAQFCNFALIETKNRALEMIKGLEAELTFTRPVRIHWTGCPNSCGQPQVADIGLMGTKARKNGKA
VEGVDIYMGGKVGKDAHLGSCVQKGIPCEDLHLVLRDLLITNFGAKPRQEALVTSQ

Sequences:

>Translated_536_residues
MTDTVTTPKASLNKFEKFKAEKDGLAIKSEIEKIASLGWEAMDATDRDHRLKWVGVFFRPVTPGKFMMRMRMPNGILTSD
QMRVLAEVVQRYGDDGNADITTRQNIQLRGIRIEDLPHIFNKFHAVGLTSVQSGMDNIRNITGDPIAGLDADELYDTREL
VQQIQDMLTNKGEGNREFSNLPRKFNIAIAGGRDNSVHAEINDLAFVPAFKEGIGDWVLGNGEESSTYQKVFGFNVLVGG
FFSAKRCEAAIPLNAWVTPEEVLPLCRAILEVYRDNGLRANRLKSRLMWLIDEWGIDKFRAEVEQRLGKSLLPAAPKDEI
DWEKRDHIGVYKQKQEGLNYVGLHIPVGRLYAEDMFELARIADVYGSGEIRMTVEQNIIIPNITDSRLRTLLTDPLLERF
SLDPGALTRSLVSCTGAQFCNFALIETKNRALEMIKGLEAELTFTRPVRIHWTGCPNSCGQPQVADIGLMGTKARKNGKA
VEGVDIYMGGKVGKDAHLGSCVQKGIPCEDLHLVLRDLLITNFGAKPRQEALVTSQ
>Mature_535_residues
TDTVTTPKASLNKFEKFKAEKDGLAIKSEIEKIASLGWEAMDATDRDHRLKWVGVFFRPVTPGKFMMRMRMPNGILTSDQ
MRVLAEVVQRYGDDGNADITTRQNIQLRGIRIEDLPHIFNKFHAVGLTSVQSGMDNIRNITGDPIAGLDADELYDTRELV
QQIQDMLTNKGEGNREFSNLPRKFNIAIAGGRDNSVHAEINDLAFVPAFKEGIGDWVLGNGEESSTYQKVFGFNVLVGGF
FSAKRCEAAIPLNAWVTPEEVLPLCRAILEVYRDNGLRANRLKSRLMWLIDEWGIDKFRAEVEQRLGKSLLPAAPKDEID
WEKRDHIGVYKQKQEGLNYVGLHIPVGRLYAEDMFELARIADVYGSGEIRMTVEQNIIIPNITDSRLRTLLTDPLLERFS
LDPGALTRSLVSCTGAQFCNFALIETKNRALEMIKGLEAELTFTRPVRIHWTGCPNSCGQPQVADIGLMGTKARKNGKAV
EGVDIYMGGKVGKDAHLGSCVQKGIPCEDLHLVLRDLLITNFGAKPRQEALVTSQ

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=470, Percent_Identity=23.8297872340426, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322597, Length=493, Percent_Identity=25.1521298174442, Blast_Score=110, Evalue=5e-25,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 59893; Mature: 59761

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTVTTPKASLNKFEKFKAEKDGLAIKSEIEKIASLGWEAMDATDRDHRLKWVGVFFRP
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEEEEC
VTPGKFMMRMRMPNGILTSDQMRVLAEVVQRYGDDGNADITTRQNIQLRGIRIEDLPHIF
CCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEHHHHHHH
NKFHAVGLTSVQSGMDNIRNITGDPIAGLDADELYDTRELVQQIQDMLTNKGEGNREFSN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
LPRKFNIAIAGGRDNSVHAEINDLAFVPAFKEGIGDWVLGNGEESSTYQKVFGFNVLVGG
CCCEEEEEEECCCCCCEEEECCCEEECCHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHC
FFSAKRCEAAIPLNAWVTPEEVLPLCRAILEVYRDNGLRANRLKSRLMWLIDEWGIDKFR
CCCHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
AEVEQRLGKSLLPAAPKDEIDWEKRDHIGVYKQKQEGLNYVGLHIPVGRLYAEDMFELAR
HHHHHHHCHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
IADVYGSGEIRMTVEQNIIIPNITDSRLRTLLTDPLLERFSLDPGALTRSLVSCTGAQFC
HHHHCCCCEEEEEEECCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHH
NFALIETKNRALEMIKGLEAELTFTRPVRIHWTGCPNSCGQPQVADIGLMGTKARKNGKA
CEEEEECHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCC
VEGVDIYMGGKVGKDAHLGSCVQKGIPCEDLHLVLRDLLITNFGAKPRQEALVTSQ
CCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
TDTVTTPKASLNKFEKFKAEKDGLAIKSEIEKIASLGWEAMDATDRDHRLKWVGVFFRP
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEEEEC
VTPGKFMMRMRMPNGILTSDQMRVLAEVVQRYGDDGNADITTRQNIQLRGIRIEDLPHIF
CCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEHHHHHHH
NKFHAVGLTSVQSGMDNIRNITGDPIAGLDADELYDTRELVQQIQDMLTNKGEGNREFSN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
LPRKFNIAIAGGRDNSVHAEINDLAFVPAFKEGIGDWVLGNGEESSTYQKVFGFNVLVGG
CCCEEEEEEECCCCCCEEEECCCEEECCHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHC
FFSAKRCEAAIPLNAWVTPEEVLPLCRAILEVYRDNGLRANRLKSRLMWLIDEWGIDKFR
CCCHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
AEVEQRLGKSLLPAAPKDEIDWEKRDHIGVYKQKQEGLNYVGLHIPVGRLYAEDMFELAR
HHHHHHHCHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
IADVYGSGEIRMTVEQNIIIPNITDSRLRTLLTDPLLERFSLDPGALTRSLVSCTGAQFC
HHHHCCCCEEEEEEECCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHH
NFALIETKNRALEMIKGLEAELTFTRPVRIHWTGCPNSCGQPQVADIGLMGTKARKNGKA
CEEEEECHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCC
VEGVDIYMGGKVGKDAHLGSCVQKGIPCEDLHLVLRDLLITNFGAKPRQEALVTSQ
CCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766873 [H]