Click here to switch to the map view.

The map label for this gene is lacA [C]

Identifier: 17228051

GI number: 17228051

Start: 646398

End: 646952

Strand: Direct

Name: lacA [C]

Synonym: alr0555

Alternate gene names: 17228051

Gene position: 646398-646952 (Clockwise)

Preceding gene: 17228050

Following gene: 17228052

Centisome position: 10.08

GC content: 32.79

Gene sequence:

>555_bases
ATGGGAAATGATGATAAATATGGTATCTCAGGTGTGACAACTTTTGAATATACCAAAATATTGGGATTAGAAAATATTGA
ATTTGGCAATAACATCATAATTGATGATTTTGTTTTTATTTATGCTAAAAAGCCTATAAAAATAGGTAACTATGTACATA
TTGCATCTTTTGCTTCAATCACAGGAGGTGAAAGATTGGTTATGGAAGATTTCACAGCCTTATCTTCAGGATCAAGAATT
TTAACAGGCACAGATGACTTTAAAGAGTGGGGTTTTGGAAATTCAACTATTAGGGAAAAATATAGAAATACAAAAAGAAA
GCCAGTACATATAGGTAAGTTCTGCATAATTGGAGCAAATAGCGTAATTTTACCAGGTGTGAATGTAGGTGAAGGCGCTA
CGATTGGAGCAGGTTCAGTTGTTACTAAAGACTTAGCTCCTTGGGGAATATATCTAGGAAATCGCAAAATAGGTGACAGA
GATAAGTCAGGTGTGCTTAAAAATTACAATAACTTTTTATTAGAAAATAACCCATCCAAGGAGATTTACTGTTAA

Upstream 100 bases:

>100_bases
GAAGAAAAATGTTCAGTAAGTAATTGCTAAGTAGTAACTATCTATGTTGAATCATCATAATGATATCTAGCTTAATTATT
CAAATTAAAGGTGTAAAGAT

Downstream 100 bases:

>100_bases
TTAAATTGGATATAATTTTCGCGGAATATTTTTAAGTATGCTGATACTGATTGATGTTCACGTATTTTTTTAATATCTGA
AAAAACGAATAAGTATCTTC

Product: hypothetical protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: Transferase Hexapeptide Repeat Containing Protein; Hexapaptide Repeat-Containing Transferase; TDP-4-Oxo-6-Deoxy-D-Glucose Transaminase; Galactoside O-Acetyltransferase; O-Acetyltransferase; Acetyltransferase RfbO CysE/LacA/LpxA/NodL Family; CysE/LacA/LpxA/NodL Family O-Acyltransferase; LPS Biosynthesis O-Acetyl Transferase; Pseudogene; Lipopolysaccharide Biosynthesis O-Acetyl Transferase; DTDP-Viosamine N-Acetyltransferase

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MGNDDKYGISGVTTFEYTKILGLENIEFGNNIIIDDFVFIYAKKPIKIGNYVHIASFASITGGERLVMEDFTALSSGSRI
LTGTDDFKEWGFGNSTIREKYRNTKRKPVHIGKFCIIGANSVILPGVNVGEGATIGAGSVVTKDLAPWGIYLGNRKIGDR
DKSGVLKNYNNFLLENNPSKEIYC

Sequences:

>Translated_184_residues
MGNDDKYGISGVTTFEYTKILGLENIEFGNNIIIDDFVFIYAKKPIKIGNYVHIASFASITGGERLVMEDFTALSSGSRI
LTGTDDFKEWGFGNSTIREKYRNTKRKPVHIGKFCIIGANSVILPGVNVGEGATIGAGSVVTKDLAPWGIYLGNRKIGDR
DKSGVLKNYNNFLLENNPSKEIYC
>Mature_183_residues
GNDDKYGISGVTTFEYTKILGLENIEFGNNIIIDDFVFIYAKKPIKIGNYVHIASFASITGGERLVMEDFTALSSGSRIL
TGTDDFKEWGFGNSTIREKYRNTKRKPVHIGKFCIIGANSVILPGVNVGEGATIGAGSVVTKDLAPWGIYLGNRKIGDRD
KSGVLKNYNNFLLENNPSKEIYC

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20228; Mature: 20097

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNDDKYGISGVTTFEYTKILGLENIEFGNNIIIDDFVFIYAKKPIKIGNYVHIASFASI
CCCCCCCCCCEEEEEHEEEEECCCCEECCCEEEEEEEEEEEECCCEEECCEEEEEEEEEC
TGGERLVMEDFTALSSGSRILTGTDDFKEWGFGNSTIREKYRNTKRKPVHIGKFCIIGAN
CCCCEEEEHHHHHHCCCCEEEECCCCHHHCCCCCHHHHHHHHCCCCCCEEECCEEEEECC
SVILPGVNVGEGATIGAGSVVTKDLAPWGIYLGNRKIGDRDKSGVLKNYNNFLLENNPSK
EEEECCCCCCCCCEECCCCEEECCCCCCEEEECCCCCCCCCCCCCHHCCCCEEEECCCCC
EIYC
CCCC
>Mature Secondary Structure 
GNDDKYGISGVTTFEYTKILGLENIEFGNNIIIDDFVFIYAKKPIKIGNYVHIASFASI
CCCCCCCCCEEEEEHEEEEECCCCEECCCEEEEEEEEEEEECCCEEECCEEEEEEEEEC
TGGERLVMEDFTALSSGSRILTGTDDFKEWGFGNSTIREKYRNTKRKPVHIGKFCIIGAN
CCCCEEEEHHHHHHCCCCEEEECCCCHHHCCCCCHHHHHHHHCCCCCCEEECCEEEEECC
SVILPGVNVGEGATIGAGSVVTKDLAPWGIYLGNRKIGDRDKSGVLKNYNNFLLENNPSK
EEEECCCCCCCCCEECCCCEEECCCCCCEEEECCCCCCCCCCCCCHHCCCCEEEECCCCC
EIYC
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA