Click here to switch to the map view.

The map label for this gene is menD [H]

Identifier: 17227808

GI number: 17227808

Start: 356416

End: 358179

Strand: Direct

Name: menD [H]

Synonym: alr0312

Alternate gene names: 17227808

Gene position: 356416-358179 (Clockwise)

Preceding gene: 17227805

Following gene: 17227813

Centisome position: 5.56

GC content: 42.97

Gene sequence:

>1764_bases
ATGTCTAATTTGATGCCGATCGCCTATAAAAATATTAATCAACTTTGGGCTTATATTTTCACCGAAACACTCAAGCGGTT
GGGATTGGCTTATGCCGTAATTTGCCCTGGTTCTCGTTCCACACCCTTAGCTGTGGCCTTTGCCCAACAAGCACCCAATA
TTGAAGCAATTTCCATACTAGATGAGCGTTCTGCCGCTTTTTTCGCCTTGGGTATAGCTAAAGCAACTAATCGTCCTGTA
GCGATTGTCTGCACTTCGGGAACTGCGGGGGCAAATTTTTATCCAGCAGTCATTGAGGCTCAAGAAAGTCGTGTACCACT
GTTATTATTAACCGCCGATAGACCCCCAGAACTAAGAGATTGTCACTCTGGACAAACTATAGATCAAATGAAACTCTATG
GTAGTTACCCAAATTGGCAAGCAGAGTTAGCTTTACCAGTATCAGATATGGGAATGCTAGCTTATCTGCGACAAACGCTA
GTTCATAGTTGGTACAGGATGCAAGCGCCAACACCGGGGCCAGTACATTTAAATATCCCCTTTCGTGACCCCCTTGCACC
GATTCCTGATGGCACAGATTTGAGCTATTTACTGGCTAAGTTTCACCCAGAAGAATTTTTTGCGGGAATAACAGATACTA
CCCCACTCCCTCATCATTCCCCACTCTCCATTCCTCCAGAATGGTTACAATCACAGCGAGGAATCATCATTGCTGGTGTC
GCTCAACCACAGCAACCACAAGAATATTGTAGAGCGATCGCCCGACTTTCCCAAACTCTACAATGGCCTGTGTTAGCTGA
GGGACTTTCCCCAGTCAGAAATTACGCTGACTTCAACCCCTATTTAATTTCCACCTACGACTTGATTTTACGCAATCAAC
AATTAGCAACACGGCTAGCACCAGATATGGTAATTCAAATAGGTGATATGCCAACGAGTAAAGAACTGCGTAATTGGATA
GACACTCACCAACCCCGACGCTGGGTAATTGATCCCAGTGACCAAAACCTCGACCCTTTGCATGGAAGGACGACCCATTT
AAGAATTAGGGTGGAAGAGTTGGGATATCAGGGGGTAGAGGAAGACAAATCTTCTGTCTCAGAGTATCTACAATTGTGGT
GTAATGCTGAAACCAAAGTTAGGGTCAATGTAGATGAGACTTTGGACAAGATGGAAGACTTGGTAGAGTGTAAAGCCGCC
TGGTTACTTTCTCAAATGCTACCGCCAGAAACGCCCTTATTTATCGCCAACAGTATGCCGGTAAGGGATGTAGAGTTTTT
CTGGAAGCCGAATAATTTAAGAGTGCGATCGCATTTTAACCGAGGCGCGAACGGTATCGATGGTACATTATCCACCGCTT
TAGGAATCTCCCACCGTCAGCAAAGTAGTGTGCTAATCACAGGGGATTTAGCTTTATTACATGACACCAACGGTTTTTTA
ATTAGAAATAAGTTTGTTGGACATTTGACAATTATCTTAATCAACAATAATGGCGGGGGAATTTTTGAAATGTTACCCAT
CGCTAAATTTGAACCGCCATTTGAAGAATTTTTTGGTACGCCCCAAGATATTGATTTTGCTCAGTTATGCACCACTTATA
ATGTCCAGCATGAATTAATTCATTCCTGGGTGCATTTACAGCAGAGATTAAACCCACTACCAAATACAGGGATTCGAGTT
TTAGAATTACGCACAAATAGAAAAATAGATGCTCAATGGCGACGTGATAATTTAAGCAATTTTGCGGCGGATAATATTAT
CTAA

Upstream 100 bases:

>100_bases
AAGGCGATACACAGCATATCTAGGTTTTGGTGGGTAATACCTACCCCACTGGGTAAAATTTCTGTACTTATGAGTTGCCA
CTAGTAATAGTATTACTGCT

Downstream 100 bases:

>100_bases
CTAAGCCATTAATCACAAGGCGTAACAGTTGCCGCTTTTGTATGGTTTTCTGGATATGAGAGAATTGGTATAATTTACTT
TTCAATTACCAATTACCCAA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MSNLMPIAYKNINQLWAYIFTETLKRLGLAYAVICPGSRSTPLAVAFAQQAPNIEAISILDERSAAFFALGIAKATNRPV
AIVCTSGTAGANFYPAVIEAQESRVPLLLLTADRPPELRDCHSGQTIDQMKLYGSYPNWQAELALPVSDMGMLAYLRQTL
VHSWYRMQAPTPGPVHLNIPFRDPLAPIPDGTDLSYLLAKFHPEEFFAGITDTTPLPHHSPLSIPPEWLQSQRGIIIAGV
AQPQQPQEYCRAIARLSQTLQWPVLAEGLSPVRNYADFNPYLISTYDLILRNQQLATRLAPDMVIQIGDMPTSKELRNWI
DTHQPRRWVIDPSDQNLDPLHGRTTHLRIRVEELGYQGVEEDKSSVSEYLQLWCNAETKVRVNVDETLDKMEDLVECKAA
WLLSQMLPPETPLFIANSMPVRDVEFFWKPNNLRVRSHFNRGANGIDGTLSTALGISHRQQSSVLITGDLALLHDTNGFL
IRNKFVGHLTIILINNNGGGIFEMLPIAKFEPPFEEFFGTPQDIDFAQLCTTYNVQHELIHSWVHLQQRLNPLPNTGIRV
LELRTNRKIDAQWRRDNLSNFAADNII

Sequences:

>Translated_587_residues
MSNLMPIAYKNINQLWAYIFTETLKRLGLAYAVICPGSRSTPLAVAFAQQAPNIEAISILDERSAAFFALGIAKATNRPV
AIVCTSGTAGANFYPAVIEAQESRVPLLLLTADRPPELRDCHSGQTIDQMKLYGSYPNWQAELALPVSDMGMLAYLRQTL
VHSWYRMQAPTPGPVHLNIPFRDPLAPIPDGTDLSYLLAKFHPEEFFAGITDTTPLPHHSPLSIPPEWLQSQRGIIIAGV
AQPQQPQEYCRAIARLSQTLQWPVLAEGLSPVRNYADFNPYLISTYDLILRNQQLATRLAPDMVIQIGDMPTSKELRNWI
DTHQPRRWVIDPSDQNLDPLHGRTTHLRIRVEELGYQGVEEDKSSVSEYLQLWCNAETKVRVNVDETLDKMEDLVECKAA
WLLSQMLPPETPLFIANSMPVRDVEFFWKPNNLRVRSHFNRGANGIDGTLSTALGISHRQQSSVLITGDLALLHDTNGFL
IRNKFVGHLTIILINNNGGGIFEMLPIAKFEPPFEEFFGTPQDIDFAQLCTTYNVQHELIHSWVHLQQRLNPLPNTGIRV
LELRTNRKIDAQWRRDNLSNFAADNII
>Mature_586_residues
SNLMPIAYKNINQLWAYIFTETLKRLGLAYAVICPGSRSTPLAVAFAQQAPNIEAISILDERSAAFFALGIAKATNRPVA
IVCTSGTAGANFYPAVIEAQESRVPLLLLTADRPPELRDCHSGQTIDQMKLYGSYPNWQAELALPVSDMGMLAYLRQTLV
HSWYRMQAPTPGPVHLNIPFRDPLAPIPDGTDLSYLLAKFHPEEFFAGITDTTPLPHHSPLSIPPEWLQSQRGIIIAGVA
QPQQPQEYCRAIARLSQTLQWPVLAEGLSPVRNYADFNPYLISTYDLILRNQQLATRLAPDMVIQIGDMPTSKELRNWID
THQPRRWVIDPSDQNLDPLHGRTTHLRIRVEELGYQGVEEDKSSVSEYLQLWCNAETKVRVNVDETLDKMEDLVECKAAW
LLSQMLPPETPLFIANSMPVRDVEFFWKPNNLRVRSHFNRGANGIDGTLSTALGISHRQQSSVLITGDLALLHDTNGFLI
RNKFVGHLTIILINNNGGGIFEMLPIAKFEPPFEEFFGTPQDIDFAQLCTTYNVQHELIHSWVHLQQRLNPLPNTGIRVL
ELRTNRKIDAQWRRDNLSNFAADNII

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=551, Percent_Identity=31.9419237749546, Blast_Score=238, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001 [H]

Pfam domain/function: PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 66176; Mature: 66044

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLMPIAYKNINQLWAYIFTETLKRLGLAYAVICPGSRSTPLAVAFAQQAPNIEAISIL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCEEEEEE
DERSAAFFALGIAKATNRPVAIVCTSGTAGANFYPAVIEAQESRVPLLLLTADRPPELRD
CCCCCEEEEEEHHHCCCCCEEEEECCCCCCCCCCHHEEECCCCCCCEEEEECCCCCCHHH
CHSGQTIDQMKLYGSYPNWQAELALPVSDMGMLAYLRQTLVHSWYRMQAPTPGPVHLNIP
CCCCCCHHHHHEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
FRDPLAPIPDGTDLSYLLAKFHPEEFFAGITDTTPLPHHSPLSIPPEWLQSQRGIIIAGV
CCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEC
AQPQQPQEYCRAIARLSQTLQWPVLAEGLSPVRNYADFNPYLISTYDLILRNQQLATRLA
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHCC
PDMVIQIGDMPTSKELRNWIDTHQPRRWVIDPSDQNLDPLHGRTTHLRIRVEELGYQGVE
CCCEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHCCCCCCH
EDKSSVSEYLQLWCNAETKVRVNVDETLDKMEDLVECKAAWLLSQMLPPETPLFIANSMP
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VRDVEFFWKPNNLRVRSHFNRGANGIDGTLSTALGISHRQQSSVLITGDLALLHDTNGFL
CCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECEEEEECCCCEE
IRNKFVGHLTIILINNNGGGIFEMLPIAKFEPPFEEFFGTPQDIDFAQLCTTYNVQHELI
EEEEEEEEEEEEEEECCCCCEEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHCCHHHHHH
HSWVHLQQRLNPLPNTGIRVLELRTNRKIDAQWRRDNLSNFAADNII
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
SNLMPIAYKNINQLWAYIFTETLKRLGLAYAVICPGSRSTPLAVAFAQQAPNIEAISIL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCEEEEEE
DERSAAFFALGIAKATNRPVAIVCTSGTAGANFYPAVIEAQESRVPLLLLTADRPPELRD
CCCCCEEEEEEHHHCCCCCEEEEECCCCCCCCCCHHEEECCCCCCCEEEEECCCCCCHHH
CHSGQTIDQMKLYGSYPNWQAELALPVSDMGMLAYLRQTLVHSWYRMQAPTPGPVHLNIP
CCCCCCHHHHHEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
FRDPLAPIPDGTDLSYLLAKFHPEEFFAGITDTTPLPHHSPLSIPPEWLQSQRGIIIAGV
CCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEC
AQPQQPQEYCRAIARLSQTLQWPVLAEGLSPVRNYADFNPYLISTYDLILRNQQLATRLA
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHCC
PDMVIQIGDMPTSKELRNWIDTHQPRRWVIDPSDQNLDPLHGRTTHLRIRVEELGYQGVE
CCCEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHCCCCCCH
EDKSSVSEYLQLWCNAETKVRVNVDETLDKMEDLVECKAAWLLSQMLPPETPLFIANSMP
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VRDVEFFWKPNNLRVRSHFNRGANGIDGTLSTALGISHRQQSSVLITGDLALLHDTNGFL
CCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECEEEEECCCCEE
IRNKFVGHLTIILINNNGGGIFEMLPIAKFEPPFEEFFGTPQDIDFAQLCTTYNVQHELI
EEEEEEEEEEEEEEECCCCCEEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHCCHHHHHH
HSWVHLQQRLNPLPNTGIRVLELRTNRKIDAQWRRDNLSNFAADNII
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA