Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 17227550

GI number: 17227550

Start: 58472

End: 59791

Strand: Direct

Name: dacB [H]

Synonym: alr0054

Alternate gene names: 17227550

Gene position: 58472-59791 (Clockwise)

Preceding gene: 17227549

Following gene: 17227551

Centisome position: 0.91

GC content: 46.52

Gene sequence:

>1320_bases
ATGCTGGAATTGTTGAGTTCGGGTTTGGTTTCTATCTGGCTGGAAATGGCTGGTGTGCAAATCAAGCCTGCGGATGCTTT
AGATGCGCTAACTTGGCAAAGTAGCCCAGGCTTGGTTATTGCACCTGATCCGAATCCAGCAGGAATTAATACAGTCAAGG
AATATCTGAAGGGATTAGTCACGACAAAATTAATTGCTCAGAATTTAGCAGAAAGCCAAGGAATTTGGTTGCAGTCAGGG
CCGATGTTGATGGCTAATCATCAAGGAACCACTCCTTTGCCGGCTGCTTCTCTCACTAAAATTGCTACTTCACTAGCGGC
CTTTACTACTTTCGGCCCAGAACATCAATTTGAAACGACGATCGCCACTAATGCTTCAGTTGTTAATGGCGTATTACAAG
GTGATTTAATCATTAATGGCGGCGGCGACCCGATGTTTGTTTGGGAAGAAGCGATCGCCCTGGGTAATACGCTCAATAAA
ATGGGAATCAAGCAGGTAAAGGGAAATTTGGTAATTACAGGCAATTTCGCCATGAATTTCCAGCGCCATCCCCTATTAGC
TGGGCAAATGCTCAAACAATCACTAAATTCTAAAACCTGGACTCGTCCAGCAAATTATATACATTCCATTATGCCCAAGG
GGACACCAAAGCCCCAGGTAGCTATTAACGGCACAATCAAATTTGCTGCCCAGGCTAACCCCAAACAAACTTTGCTAGTG
CGTCACCGTTCCTTACCCTTGAAGCAATTAATCAAGGAGATGAATGTTTATAGTAATAATGAGATGGCGCAGATGTTGGC
AGAAGCAGTGGGAGGCCATGCTGTAGTCCAAGCCAAAGCCGCTAAATTAGCGCGAGTACCAGAGACAGAAATTCAATTAA
TCAATGGTTCGGGACTTGGCCCGGAAAATCGGATTTCTCCCAGGGCGGCTTGTGCGATGTTGATGGCGCTGCAACATGAA
GCGGCGGCGGCTCAACTCAATCTAGCGGATTTGTTTCCCATTGCTGGCTTTGATAAACGAGGCACAATGCACTCTCGACA
TTTGCCGATGGCTACTGTGATGAAAACCGGGACTTTGCGGGATGTGAGTGCTTTAGCTGGAGTTGTACCCACACGCAATC
GCGGTTTGGTTTGGTTCGCTATCATTAACCGTGGCCCTCAAATTGGCGCTTTCCGTCAACAACAAGACAAACTTTTACAA
CGTCTAGTACAGCAATTACAAGTAGCCCCTAGCACACCCACAGCTCTTACCCCCCATTCACCTAAATCTTTACCCGATCT
GGGGAATACTAACCGCAGTGAAATTGTGTATGGTGGCTAG

Upstream 100 bases:

>100_bases
AAAAAATACCAGTTATGATAACTCAATGCTGATGAACATCATAAAGCGCGTATATTAGCACAGGAAACAATATATTACAG
TCATAAGTGGGGAGTGAATC

Downstream 100 bases:

>100_bases
TAGCGTAATTCGTAATTATGCTGTTGTGAGGGTTATAAACTTTGCCGATAGGCGTTTATTTACACTGCAACTAGTATCAC
TACACGAAATTAAGCAGGTA

Product: hypothetical protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLVTTKLIAQNLAESQGIWLQSG
PMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETTIATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNK
MGIKQVKGNLVITGNFAMNFQRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVAINGTIKFAAQANPKQTLLV
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHAVVQAKAAKLARVPETEIQLINGSGLGPENRISPRAACAMLMALQHE
AAAAQLNLADLFPIAGFDKRGTMHSRHLPMATVMKTGTLRDVSALAGVVPTRNRGLVWFAIINRGPQIGAFRQQQDKLLQ
RLVQQLQVAPSTPTALTPHSPKSLPDLGNTNRSEIVYGG

Sequences:

>Translated_439_residues
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLVTTKLIAQNLAESQGIWLQSG
PMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETTIATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNK
MGIKQVKGNLVITGNFAMNFQRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVAINGTIKFAAQANPKQTLLV
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHAVVQAKAAKLARVPETEIQLINGSGLGPENRISPRAACAMLMALQHE
AAAAQLNLADLFPIAGFDKRGTMHSRHLPMATVMKTGTLRDVSALAGVVPTRNRGLVWFAIINRGPQIGAFRQQQDKLLQ
RLVQQLQVAPSTPTALTPHSPKSLPDLGNTNRSEIVYGG
>Mature_439_residues
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLVTTKLIAQNLAESQGIWLQSG
PMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETTIATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNK
MGIKQVKGNLVITGNFAMNFQRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVAINGTIKFAAQANPKQTLLV
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHAVVQAKAAKLARVPETEIQLINGSGLGPENRISPRAACAMLMALQHE
AAAAQLNLADLFPIAGFDKRGTMHSRHLPMATVMKTGTLRDVSALAGVVPTRNRGLVWFAIINRGPQIGAFRQQQDKLLQ
RLVQQLQVAPSTPTALTPHSPKSLPDLGNTNRSEIVYGG

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=98, Percent_Identity=36.734693877551, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47081; Mature: 47081

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLV
CHHHHHHHHHHHHHHHHCCEECCCHHHHHHEECCCCCEEEECCCCCCCHHHHHHHHHHHH
TTKLIAQNLAESQGIWLQSGPMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETT
HHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
IATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNKMGIKQVKGNLVITGNFAMNF
EECCHHHHHHHEECCEEEECCCCCEEHHHHHHHHCCHHHHHHHHHHCCCEEEEECEEECC
QRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVAINGTIKFAAQANPKQTLLV
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECEEEEEECCCCCCEEEE
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHAVVQAKAAKLARVPETEIQLINGSGLG
HHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCC
PENRISPRAACAMLMALQHEAAAAQLNLADLFPIAGFDKRGTMHSRHLPMATVMKTGTLR
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCH
DVSALAGVVPTRNRGLVWFAIINRGPQIGAFRQQQDKLLQRLVQQLQVAPSTPTALTPHS
HHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKSLPDLGNTNRSEIVYGG
CCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLV
CHHHHHHHHHHHHHHHHCCEECCCHHHHHHEECCCCCEEEECCCCCCCHHHHHHHHHHHH
TTKLIAQNLAESQGIWLQSGPMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETT
HHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
IATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNKMGIKQVKGNLVITGNFAMNF
EECCHHHHHHHEECCEEEECCCCCEEHHHHHHHHCCHHHHHHHHHHCCCEEEEECEEECC
QRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVAINGTIKFAAQANPKQTLLV
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECEEEEEECCCCCCEEEE
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHAVVQAKAAKLARVPETEIQLINGSGLG
HHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCC
PENRISPRAACAMLMALQHEAAAAQLNLADLFPIAGFDKRGTMHSRHLPMATVMKTGTLR
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCH
DVSALAGVVPTRNRGLVWFAIINRGPQIGAFRQQQDKLLQRLVQQLQVAPSTPTALTPHS
HHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKSLPDLGNTNRSEIVYGG
CCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]