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The map label for this gene is rfbB [H]

Identifier: 17227534

GI number: 17227534

Start: 39857

End: 40927

Strand: Direct

Name: rfbB [H]

Synonym: alr0038

Alternate gene names: 17227534

Gene position: 39857-40927 (Clockwise)

Preceding gene: 17227531

Following gene: 17227535

Centisome position: 0.62

GC content: 43.32

Gene sequence:

>1071_bases
ATGATACAAAATGATACAGAACTATTGACAACATCTGTTGCGGCTATGCCTATTCTGATTACAGGTGGTGCAGGCTTTAT
TGGTTCCAATTTCGTCCATCACTGGTATGAAAAGTACCCAGGCGATCGCATGATTGTTTTGGACGCGCTCACCTATGCTG
GTAATCGCCAAAATTTAGCAGACATAGAAGGAAAAGCAAATTTACGTTTTGTAAAGGGAGATATAGGCGATCGGGCCCTT
ATAGATCAGTTACTAGAGGAAGAAAAGATTCAGGCGATCGCCCACTTTGCAGCCGAATCTCATGTTGATCGCTCAATTGT
CGCACCAGATGCTTTCATTCAGACCAATGTAGTGGGTACATTCACCTTATTAGAAGCTTTTCGCCATCACTGGACAAAAC
AAGGCAAACCTGCAAACTACCGCTTTCTCCACGTCTCTACAGATGAAGTTTACGGCAGCCTTGAACTAGATGACCCAGCT
TTTACAGAAACAACTCCTTACGCACCCAACAGTCCCTATTCCGCCTCCAAAGCAGGTAGTGATCATCTAGCACGAGCTTA
TTATCATACCTATGGTTTACCAACCTTAATTACCAACTGCTCCAATAACTACGGCCCCTATCACTTCCCCGAAAAATTAA
TACCCTTGATTTGTCTAAATATTCTCTTAGGTAAGCCTCTACCCATCTATGGAGATGGGTTAAATATCCGCGATTGGCTT
TATGTTGAAGATCATTGCCGTGCTTTAGATATTGTGATTCATCAAGGTAAGCCAGGAGAAACCTATAACATTGGCGGTAA
TAACGAAATCAAAAACATTGACCTTGTTCAGATGATCTGTGAGTTAATGGACGAATTAGCCCCTGATTTACCCGTTTCTC
CCGCCAGTAAACTAATTACCTTCGTCAAAGACCGCCCCGGACACGATCGCCGTTATGCGATCAACGCAACAAAAATCAAA
ACAGAATTAGGTTGGGAACCTCAACAAACAATCTCGACTGGATTACGCCACACCATCCAGTGGTATCTAACTCATCGCCA
TTGGTGGGAAGCACTTTTACCAAAGGAGTAG

Upstream 100 bases:

>100_bases
AGCCTAAAGATGGAAGATTTTGTCAACGGTAAACTTTATTGATATTGTTTCTTGTGAATTCTGCGGTGTTTTCTGCCCAA
CTCAAGCAAAAATAAAATCC

Downstream 100 bases:

>100_bases
GTCAAGATTGAAAGGGAAAAATCATGTTCACCAACTTTGAACAAACCATAGTTGATACAACTGAGGCTCGAATCAATCTA
GTCAAAGCTGGTCACGGCGC

Product: dTDP-glucose 4-6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MIQNDTELLTTSVAAMPILITGGAGFIGSNFVHHWYEKYPGDRMIVLDALTYAGNRQNLADIEGKANLRFVKGDIGDRAL
IDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGTFTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPA
FTETTPYAPNSPYSASKAGSDHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLITFVKDRPGHDRRYAINATKIK
TELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE

Sequences:

>Translated_356_residues
MIQNDTELLTTSVAAMPILITGGAGFIGSNFVHHWYEKYPGDRMIVLDALTYAGNRQNLADIEGKANLRFVKGDIGDRAL
IDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGTFTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPA
FTETTPYAPNSPYSASKAGSDHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLITFVKDRPGHDRRYAINATKIK
TELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE
>Mature_356_residues
MIQNDTELLTTSVAAMPILITGGAGFIGSNFVHHWYEKYPGDRMIVLDALTYAGNRQNLADIEGKANLRFVKGDIGDRAL
IDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGTFTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPA
FTETTPYAPNSPYSASKAGSDHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLITFVKDRPGHDRRYAINATKIK
TELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=38.5756676557863, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=24.5454545454545, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=60.7669616519174, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1788353, Length=343, Percent_Identity=57.1428571428571, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786974, Length=364, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788366, Length=284, Percent_Identity=25, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=37.5, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI115532424, Length=334, Percent_Identity=33.5329341317365, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=25.3012048192771, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=281, Percent_Identity=25.2669039145907, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=281, Percent_Identity=24.9110320284698, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=281, Percent_Identity=24.9110320284698, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=281, Percent_Identity=24.9110320284698, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=281, Percent_Identity=24.9110320284698, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=281, Percent_Identity=24.9110320284698, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=268, Percent_Identity=25.7462686567164, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=26.3636363636364, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=26.4623955431755, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40135; Mature: 40135

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQNDTELLTTSVAAMPILITGGAGFIGSNFVHHWYEKYPGDRMIVLDALTYAGNRQNLA
CCCCCHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCH
DIEGKANLRFVKGDIGDRALIDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGT
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHCHHHH
FTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPAFTETTPYAPNSPYSASKAGS
HHHHHHHHHHHHCCCCCCCEEEEEEEHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
DHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEE
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLIT
EECCCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
FVKDRPGHDRRYAINATKIKTELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE
HHHCCCCCCCEEEEEHEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIQNDTELLTTSVAAMPILITGGAGFIGSNFVHHWYEKYPGDRMIVLDALTYAGNRQNLA
CCCCCHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCH
DIEGKANLRFVKGDIGDRALIDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGT
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHCHHHH
FTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPAFTETTPYAPNSPYSASKAGS
HHHHHHHHHHHHCCCCCCCEEEEEEEHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
DHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEE
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLIT
EECCCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
FVKDRPGHDRRYAINATKIKTELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE
HHHCCCCCCCEEEEEHEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]