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The map label for this gene is menE [H]

Identifier: 17227531

GI number: 17227531

Start: 35433

End: 36884

Strand: Direct

Name: menE [H]

Synonym: alr0035

Alternate gene names: 17227531

Gene position: 35433-36884 (Clockwise)

Preceding gene: 17227530

Following gene: 17227534

Centisome position: 0.55

GC content: 40.91

Gene sequence:

>1452_bases
ATGGAACAACCCTTAGCCTATCTGAATCGCCTCACTCACCATGATTGGCTGATTGGTTATGACAGCCGAGAATTTCACCA
AAAAGCCACCGAACTCTACTTACAACTTAGTCAATTATCAACTCCAGAGACATCATCAAAAATCATCCTAGCCGAGCGTG
AATCCGTAAAATTTCTAGCTAGTTTTATCGCCGCCTGTGCTGCTAATTGTCAAGTTTTCCTCTGTAACCCAGACTGGGGC
GAACAAGAATGGCATCAAGTATTTAATTTAGTCCAACCCGATATTATTTTAGGCTTAGAGACTAGAGAGGGAATCTTAAG
AGGCAGGGGGGCAGGGAGCGGGGAGCAGGGGGAAAATATCTTACTAACTCAGCACGCTGCTCAACTCCCGTTACCACTCG
AAGCGCAGCTATGCCCCCAAGGCTTTACAGCACGCATCATGATTCCCACCGGTGGTTCATCGGGACAAATTAAATTTGCC
ATCCACACTTGGGAAACCCTCACCGCTTCAGTGCAGGGATTTACAGAATATTTTCAAATCAAACAAGTTAACTCATATTG
TGTTTTACCGCTATATCATGTCAGTGGTTTAATGCAGTTTATGCGTTCTTTTACCACAGGCGGTAAGCTAGTAATTCAAC
CATTTAAATCATTAGAACTCAGTCAAATATCCCAAATTAACCCTACAGAATTTTTCATATCCTTAGTACCGACACAGTTA
CAACGCCTATTACAAAACTTAGAATTAACTACATGGTTATCACAATTCAACACCGTACTTTTAGGTGGTGCGCCGGCTTG
GGATGAATTATTAGAAAAAGCCAAATTCCATAACATTCGATTAGCGCCCACCTATGGAATGACAGAAACCGCCTCCCAAA
TTGCCACCCTTAAACCAGATGATTTTTTGAATGGTAAAATCAACAGTGGTCAAATTCTTCCCCATGCCCAAGTGACAATT
TGTAATCAACAAGGGGAAGTTCTACCAGCAAATCACATAGGAAATATCACAATTCAAACTCAATCTTTATCCCTTGGTTA
TTATCCCCAAATTGTACAGAAACAAAATTATTTACAGGTAGATGATTTAGGTTTTTTAGATGAGCATGGTTATTTAAATA
TTGTCGGCAGAAACAGCGACAAAATCATCACAGGTGGAGAAAACATCTACCCCATAGAAATAGAATCAGCCATCCGCGCT
ACACAAATGGTAGCTGATGTTTGCGTAATTGGCATACCAGATAAGCAATGGGGACAAGCATTAACAGCAATTTATATTCC
CAAAGACACCAATACATCCATCAGTGAAATCAAAACTGCCATCTCCCATCAACTCAGCAGATTTAAAATCCCCAAACATT
GGATTCCATTAAATACCTTACCCCGTAACCCCCAAGGTAAAATCAATCGCCAACAACTACAACAAATAGCTGTGTTAAGC
ATAGGGGCATAG

Upstream 100 bases:

>100_bases
CACTCCAACTAGCAGCAGAATTATCTCGTGACAACAGGGCTGTTGGTTTTGGCGTTAACCACCTTTTTCACCAAGAAGCA
GAAAACTGGCTGCAAAGTTT

Downstream 100 bases:

>100_bases
ATAAGCAGTAGAGGGGAGAAATCCTCTTAAAAAAAACTCCGCGTACCTCCGCGCCTCCCTCCGCGCCCCTCTGCGTTTAA
AAACACTCTACCTAATTAGA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MEQPLAYLNRLTHHDWLIGYDSREFHQKATELYLQLSQLSTPETSSKIILAERESVKFLASFIAACAANCQVFLCNPDWG
EQEWHQVFNLVQPDIILGLETREGILRGRGAGSGEQGENILLTQHAAQLPLPLEAQLCPQGFTARIMIPTGGSSGQIKFA
IHTWETLTASVQGFTEYFQIKQVNSYCVLPLYHVSGLMQFMRSFTTGGKLVIQPFKSLELSQISQINPTEFFISLVPTQL
QRLLQNLELTTWLSQFNTVLLGGAPAWDELLEKAKFHNIRLAPTYGMTETASQIATLKPDDFLNGKINSGQILPHAQVTI
CNQQGEVLPANHIGNITIQTQSLSLGYYPQIVQKQNYLQVDDLGFLDEHGYLNIVGRNSDKIITGGENIYPIEIESAIRA
TQMVADVCVIGIPDKQWGQALTAIYIPKDTNTSISEIKTAISHQLSRFKIPKHWIPLNTLPRNPQGKINRQQLQQIAVLS
IGA

Sequences:

>Translated_483_residues
MEQPLAYLNRLTHHDWLIGYDSREFHQKATELYLQLSQLSTPETSSKIILAERESVKFLASFIAACAANCQVFLCNPDWG
EQEWHQVFNLVQPDIILGLETREGILRGRGAGSGEQGENILLTQHAAQLPLPLEAQLCPQGFTARIMIPTGGSSGQIKFA
IHTWETLTASVQGFTEYFQIKQVNSYCVLPLYHVSGLMQFMRSFTTGGKLVIQPFKSLELSQISQINPTEFFISLVPTQL
QRLLQNLELTTWLSQFNTVLLGGAPAWDELLEKAKFHNIRLAPTYGMTETASQIATLKPDDFLNGKINSGQILPHAQVTI
CNQQGEVLPANHIGNITIQTQSLSLGYYPQIVQKQNYLQVDDLGFLDEHGYLNIVGRNSDKIITGGENIYPIEIESAIRA
TQMVADVCVIGIPDKQWGQALTAIYIPKDTNTSISEIKTAISHQLSRFKIPKHWIPLNTLPRNPQGKINRQQLQQIAVLS
IGA
>Mature_483_residues
MEQPLAYLNRLTHHDWLIGYDSREFHQKATELYLQLSQLSTPETSSKIILAERESVKFLASFIAACAANCQVFLCNPDWG
EQEWHQVFNLVQPDIILGLETREGILRGRGAGSGEQGENILLTQHAAQLPLPLEAQLCPQGFTARIMIPTGGSSGQIKFA
IHTWETLTASVQGFTEYFQIKQVNSYCVLPLYHVSGLMQFMRSFTTGGKLVIQPFKSLELSQISQINPTEFFISLVPTQL
QRLLQNLELTTWLSQFNTVLLGGAPAWDELLEKAKFHNIRLAPTYGMTETASQIATLKPDDFLNGKINSGQILPHAQVTI
CNQQGEVLPANHIGNITIQTQSLSLGYYPQIVQKQNYLQVDDLGFLDEHGYLNIVGRNSDKIITGGENIYPIEIESAIRA
TQMVADVCVIGIPDKQWGQALTAIYIPKDTNTSISEIKTAISHQLSRFKIPKHWIPLNTLPRNPQGKINRQQLQQIAVLS
IGA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=304, Percent_Identity=30.9210526315789, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI42544132, Length=265, Percent_Identity=26.0377358490566, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI115511026, Length=214, Percent_Identity=28.5046728971963, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI122937307, Length=257, Percent_Identity=25.6809338521401, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI38505220, Length=260, Percent_Identity=25.7692307692308, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI157311624, Length=252, Percent_Identity=24.2063492063492, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI157311622, Length=252, Percent_Identity=24.2063492063492, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI27477105, Length=142, Percent_Identity=30.9859154929577, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788595, Length=335, Percent_Identity=30.7462686567164, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788107, Length=353, Percent_Identity=26.628895184136, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI145693145, Length=346, Percent_Identity=23.6994219653179, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI221142682, Length=206, Percent_Identity=29.6116504854369, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786810, Length=342, Percent_Identity=24.2690058479532, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=306, Percent_Identity=26.797385620915, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=304, Percent_Identity=27.9605263157895, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=347, Percent_Identity=31.1239193083574, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=473, Percent_Identity=24.7357293868922, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=353, Percent_Identity=24.3626062322946, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=352, Percent_Identity=23.5795454545455, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=353, Percent_Identity=24.3626062322946, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=307, Percent_Identity=26.7100977198697, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=301, Percent_Identity=24.9169435215947, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=311, Percent_Identity=26.0450160771704, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=345, Percent_Identity=23.768115942029, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=307, Percent_Identity=28.0130293159609, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581924, Length=313, Percent_Identity=27.1565495207668, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21355181, Length=503, Percent_Identity=22.8628230616302, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18859661, Length=311, Percent_Identity=26.6881028938907, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24653035, Length=221, Percent_Identity=27.6018099547511, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656500, Length=361, Percent_Identity=24.3767313019391, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19922652, Length=360, Percent_Identity=22.2222222222222, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356441, Length=266, Percent_Identity=27.8195488721804, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI161076582, Length=219, Percent_Identity=27.3972602739726, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648253, Length=235, Percent_Identity=25.9574468085106, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648255, Length=235, Percent_Identity=26.3829787234043, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281365686, Length=186, Percent_Identity=29.5698924731183, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20130357, Length=203, Percent_Identity=26.6009852216749, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356947, Length=246, Percent_Identity=25.609756097561, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17933690, Length=227, Percent_Identity=25.9911894273128, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 53916; Mature: 53916

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQPLAYLNRLTHHDWLIGYDSREFHQKATELYLQLSQLSTPETSSKIILAERESVKFLA
CCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHH
SFIAACAANCQVFLCNPDWGEQEWHQVFNLVQPDIILGLETREGILRGRGAGSGEQGENI
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCCCCEE
LLTQHAAQLPLPLEAQLCPQGFTARIMIPTGGSSGQIKFAIHTWETLTASVQGFTEYFQI
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECHHHHHHHHHHHHHHHE
KQVNSYCVLPLYHVSGLMQFMRSFTTGGKLVIQPFKSLELSQISQINPTEFFISLVPTQL
EECCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHH
QRLLQNLELTTWLSQFNTVLLGGAPAWDELLEKAKFHNIRLAPTYGMTETASQIATLKPD
HHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHEECCCC
DFLNGKINSGQILPHAQVTICNQQGEVLPANHIGNITIQTQSLSLGYYPQIVQKQNYLQV
CCCCCCCCCCEECCCCEEEEECCCCCEEECCCCCEEEEEEEEECCCCCHHHHCCCCCEEE
DDLGFLDEHGYLNIVGRNSDKIITGGENIYPIEIESAIRATQMVADVCVIGIPDKQWGQA
CCCCEECCCCEEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHHHEEEEECCCHHHCCE
LTAIYIPKDTNTSISEIKTAISHQLSRFKIPKHWIPLNTLPRNPQGKINRQQLQQIAVLS
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
IGA
CCC
>Mature Secondary Structure
MEQPLAYLNRLTHHDWLIGYDSREFHQKATELYLQLSQLSTPETSSKIILAERESVKFLA
CCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHH
SFIAACAANCQVFLCNPDWGEQEWHQVFNLVQPDIILGLETREGILRGRGAGSGEQGENI
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCCCCEE
LLTQHAAQLPLPLEAQLCPQGFTARIMIPTGGSSGQIKFAIHTWETLTASVQGFTEYFQI
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECHHHHHHHHHHHHHHHE
KQVNSYCVLPLYHVSGLMQFMRSFTTGGKLVIQPFKSLELSQISQINPTEFFISLVPTQL
EECCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHH
QRLLQNLELTTWLSQFNTVLLGGAPAWDELLEKAKFHNIRLAPTYGMTETASQIATLKPD
HHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHEECCCC
DFLNGKINSGQILPHAQVTICNQQGEVLPANHIGNITIQTQSLSLGYYPQIVQKQNYLQV
CCCCCCCCCCEECCCCEEEEECCCCCEEECCCCCEEEEEEEEECCCCCHHHHCCCCCEEE
DDLGFLDEHGYLNIVGRNSDKIITGGENIYPIEIESAIRATQMVADVCVIGIPDKQWGQA
CCCCEECCCCEEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHHHEEEEECCCHHHCCE
LTAIYIPKDTNTSISEIKTAISHQLSRFKIPKHWIPLNTLPRNPQGKINRQQLQQIAVLS
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
IGA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA