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The map label for this gene is purF

Identifier: 170757817

GI number: 170757817

Start: 3161122

End: 3162498

Strand: Reverse

Name: purF

Synonym: CLD_1630

Alternate gene names: 170757817

Gene position: 3162498-3161122 (Counterclockwise)

Preceding gene: 170756882

Following gene: 170756154

Centisome position: 79.9

GC content: 28.1

Gene sequence:

>1377_bases
ATGGTATTTGATAGAAAGCAGGATAAATTTAGGGAAGAATGTGGAGTCTTTGGCGTTTTTAAAGATTATACATCAGAACT
TGGAGAAATATTTTATCCAGGGTTAGTATCTCTTCAACATAGAGGAGAAGAAAGTTCAGGAATAAGCTATACAACTAGCA
AAGGTATGATAACTAAAAAAGCTCTTGGAATGGTGTCTAACTTGTTTTCTAAAGAGGATTTTTATAAAATGAAATATTTT
TCTGCTATAGGACATGTTAGATATTCAACTAGTGGTAATGCTTCTATTGAAAATGCACAGCCATTTCAAGAAGAAACTAT
AGATGGATCTATTTCTTTAGCTCATAATGGAAATTTATTAAATTATTTAAATGTTAAGTATGAATTAGAGAAAAAAGGAA
TGATATTTAAAAGTAATTCAGACTCTGAAATTATACTAAAGTTTATATTAGAAAAAATTAAAGAAGTAAGAGAAATAGAA
AAAGCCATAGCTTATGCTATTAATACTTTAAAGGGAGCTTTTTCAGTACTTATTTTAACCGAAGATAAGTTAATAGGTTT
TAGAGATAAAAATGGTATAAGACCTTTATGTCTAGGAAAAATAGAAGGCAATTATATTTTATCTTCAGAAAGTACTTCCA
TAGATGTTGTGGGAGGAGAATACATAAGAGATGTAGATCCAGGGGAAATAGTGGTTATAAATAAAAAAGGAATAAAATTC
ATAAAGAATAAAGAAGTTTATTGTGGTTCTCTCTGCGCATTAGAATATATATATTTTTCACGACCAGATAGTATCATAGA
TGGGATAAATCTATCACAATTTAGAATTAAATGTGGAGAAAAGTTATATGAAAAATACAAATTAAGATCAGATATTGTAA
TGGGAGTACCTGAATCAGGAAATTTTGCAGCTTTAGGTTATTCTAAGGAATCAAATATACCATATAGTATAGGTCTTATT
AAAAATTCTTATGTAGGTAGAAATTTTATAAAAGCTACAGAAAAGGAAAGAAAAAGGGATATAAATATAAAAATAAATGC
CATAAAAAGTATAGTTCAAGATAAAAGCGTTATTGTGATAGATGATTCTATTGTAAGAGGCACTAGTAGTAAAAAGGTAG
TGTCCGCATTAAAAAAAGCAGGGGCAAGGGAGGTTCACTTTATGGTGGCATCTCCTAAAATAAATTATTATTGTAATTTG
GGTATAGATATAAAAAGTAAAAAAGAGCTTCTATCTTTCAAAAAAACAAAGGAAGAAATGAGAAAGTTTATAGGGGCAGA
TAGCTTAGAATTTTTAAGTTTAATAGATATGGAACAGTGTTTAAATAAATCTAACATTTGTACAGGTTGTTTTGATGGAA
GTTATGCTAATTATTAA

Upstream 100 bases:

>100_bases
TATAGTAAATTATATATAGTATAAAAAGCTTACCTTCGGGTGGGCTTTATTTTTTTATTTTTAAATTATAATTTGATATT
TTAAGTTAGAAGGGATGCAA

Downstream 100 bases:

>100_bases
ATTTCATAGCTAGTATAGTATACATAAAAATTTATAATATAAAATAAATTTAATTAGTTTTTAACATTGATTTCTTTTTA
CTTTCTAGAGGTAATATTTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MVFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKKALGMVSNLFSKEDFYKMKYF
SAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLLNYLNVKYELEKKGMIFKSNSDSEIILKFILEKIKEVREIE
KAIAYAINTLKGAFSVLILTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKLRSDIVMGVPESGNFAALGYSKESNIPYSIGLI
KNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSVIVIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNL
GIDIKSKKELLSFKKTKEEMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY

Sequences:

>Translated_458_residues
MVFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKKALGMVSNLFSKEDFYKMKYF
SAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLLNYLNVKYELEKKGMIFKSNSDSEIILKFILEKIKEVREIE
KAIAYAINTLKGAFSVLILTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKLRSDIVMGVPESGNFAALGYSKESNIPYSIGLI
KNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSVIVIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNL
GIDIKSKKELLSFKKTKEEMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY
>Mature_458_residues
MVFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKKALGMVSNLFSKEDFYKMKYF
SAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLLNYLNVKYELEKKGMIFKSNSDSEIILKFILEKIKEVREIE
KAIAYAINTLKGAFSVLILTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKLRSDIVMGVPESGNFAALGYSKESNIPYSIGLI
KNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSVIVIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNL
GIDIKSKKELLSFKKTKEEMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=33.9250493096647, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1788651, Length=465, Percent_Identity=35.6989247311828, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1790167, Length=229, Percent_Identity=27.0742358078603, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554892, Length=477, Percent_Identity=35.0104821802935, Blast_Score=272, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6323958, Length=470, Percent_Identity=34.6808510638298, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI28573187, Length=487, Percent_Identity=35.7289527720739, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24659598, Length=483, Percent_Identity=35.6107660455487, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24659604, Length=457, Percent_Identity=35.4485776805252, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI21357745, Length=182, Percent_Identity=29.6703296703297, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51504; Mature: 51504

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKK
CCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCHHHH
ALGMVSNLFSKEDFYKMKYFSAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLL
HHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHCCCCEEEEEECCCEE
NYLNVKYELEKKGMIFKSNSDSEIILKFILEKIKEVREIEKAIAYAINTLKGAFSVLILT
EEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
EDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
CCCEEEEECCCCCCCEEEEEECCCEEEECCCCEEEEECCHHHCCCCCCCEEEEECCCCEE
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKLRSDIVMGVPESG
EECCCCEECHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
NFAALGYSKESNIPYSIGLIKNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSVIVI
CEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEEE
DDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNLGIDIKSKKELLSFKKTKEEM
ECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEECEEECCHHHHHHHHHHHHHH
RKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY
HHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MVFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKK
CCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCHHHH
ALGMVSNLFSKEDFYKMKYFSAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLL
HHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHCCCCEEEEEECCCEE
NYLNVKYELEKKGMIFKSNSDSEIILKFILEKIKEVREIEKAIAYAINTLKGAFSVLILT
EEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
EDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGIKF
CCCEEEEECCCCCCCEEEEEECCCEEEECCCCEEEEECCHHHCCCCCCCEEEEECCCCEE
IKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKLRSDIVMGVPESG
EECCCCEECHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
NFAALGYSKESNIPYSIGLIKNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSVIVI
CEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEEE
DDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNLGIDIKSKKELLSFKKTKEEM
ECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEECEEECCHHHHHHHHHHHHHH
RKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY
HHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]