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The map label for this gene is thiC

Identifier: 170756154

GI number: 170756154

Start: 3159598

End: 3160911

Strand: Reverse

Name: thiC

Synonym: CLD_1631

Alternate gene names: 170756154

Gene position: 3160911-3159598 (Counterclockwise)

Preceding gene: 170757817

Following gene: 170755340

Centisome position: 79.86

GC content: 33.64

Gene sequence:

>1314_bases
ATGAATTATACAACTCAAATGGATGCTGCTAAAAAAGGAATAGTTACAAAAGAGATGGAAATAGTAGCTAAAAAGGAAAA
TATGAATGTGAAAGACCTTATGAAATTGGTATCTAAAGGTAAGGTTGCTATACCTGCTAATAAAAATCATAAATCTTTAG
ATCCAGAAGGAATAGGACAAGGTTTAAGAACTAAAATAAATGTCAATTTAGGAATATCTAAAGATTGCTATAACATAGAT
ATGGAATTAGAAAAAGTACAAAAAGCAATAGATATGAAGGCAGAGGCTATAATGGATTTGAGCTGCTTTGGAAAAACAGA
AGAATTTAGAAAAAGATTGATAGACATGTCACCAGCCATAATAGGTACTGTTCCTATATATGATGCAGTAGGTTTCTATG
ATAAAGAATTAAAAGATATTACATCAGAAGAATTTTTAAAGGTAGCAGAGAAGCATGCAGAGAATGGAGCAGACTTTTTA
ACAATACATGTAGGTATGAATAGAAAAACTGCAGCTACATTTAAGAAAAATCCAAGAAGAATGAACATAGTTTCAAGAGG
AGGATCCCTTTTATATGCATGGATGGAACTAAATAATAAAGAAAATCCATTCTATGAAGGATTTGATAAACTTTTAGATA
TATGTGAAAAGTATGATGTTACATTAAGCTTAGGGGATGCTTGTCGTCCAGGCTGTATAGAGGATTCTACAGATGCAAGC
CAGATAGAAGAGCTTATAGCCTTAGGAGAACTTACCAAAAGAGCCTGGGAGAGGAATGTACAAGTTATAATAGAGGGACC
AGGACACATGACTTTAGATGAAATAGAAACAAATATGAAAATAGAGAAAAAATTATGTCATGGAGCTCCGTTCTATGTTT
TAGGACCAATAGTTACAGATATAGCTCCAGGTTATGATCACATAACTTCAGCTATAGGTGGTGCTATTGCAGCAACTCAT
GGTGCAGATTTCTTATGCTATGTAACCCCAGCAGAGCATTTAAGATTACCAAACTTAGATGATATGAAGGAAGGTATAAT
AGCGACAAAGATAGCAGCTCATGCAGCAGATCTAGCTAAAGGAGTAAAGGGAGCAAGAGATTGGGATAATGCTATGGCTA
AAGCTAGAAGAGATTTAGACTGGGAAAGAATGTTTGAGTTATCTATAGATGAAGAAAAAGCTAGAAGATATAGAGAAGAA
TCTAAGGCAAAAAGTAAAGATTCTTGTACAATGTGCGGAAAAATGTGTGCAGTAAGAAATATGAACAGAGTTACAGAAGG
AAAAGACTTAAACATGTTAAGAGATGATGATTAA

Upstream 100 bases:

>100_bases
TCATTATATTGCAATAAACTTAGAGAAATTAATGTACATATAAATAAATAAAATCCCTGTAGCATATTTTACAGGAGATA
TGAAAAGGAGAAATGTTATT

Downstream 100 bases:

>100_bases
TGTATAGGGGTGATTATAATGGCAAATATAAATTTGAGTTTACAAGTGCTACCCGTAGTTAGTGAAGAAGAGATATATCC
AGTAGTGGATAAAGTTATAG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMKLVSKGKVAIPANKNHKSLDPEGIGQGLRTKINVNLGISKDCYNID
MELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAIIGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFL
TIHVGMNRKTAATFKKNPRRMNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAIAATH
GADFLCYVTPAEHLRLPNLDDMKEGIIATKIAAHAADLAKGVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREE
SKAKSKDSCTMCGKMCAVRNMNRVTEGKDLNMLRDDD

Sequences:

>Translated_437_residues
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMKLVSKGKVAIPANKNHKSLDPEGIGQGLRTKINVNLGISKDCYNID
MELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAIIGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFL
TIHVGMNRKTAATFKKNPRRMNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAIAATH
GADFLCYVTPAEHLRLPNLDDMKEGIIATKIAAHAADLAKGVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREE
SKAKSKDSCTMCGKMCAVRNMNRVTEGKDLNMLRDDD
>Mature_437_residues
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMKLVSKGKVAIPANKNHKSLDPEGIGQGLRTKINVNLGISKDCYNID
MELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAIIGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFL
TIHVGMNRKTAATFKKNPRRMNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAIAATH
GADFLCYVTPAEHLRLPNLDDMKEGIIATKIAAHAADLAKGVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREE
SKAKSKDSCTMCGKMCAVRNMNRVTEGKDLNMLRDDD

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=447, Percent_Identity=43.6241610738255, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 48873; Mature: 48873

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMKLVSKGKVAIPANKNHKSLDPEGIGQ
CCCCCCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHCC
GLRTKINVNLGISKDCYNIDMELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAI
CCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
IGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFLTIHVGMNRKTAATFKKNPRR
EECCHHHHHHCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCE
MNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGAIAATHGADFLCYVTPAEHLRLPNLDDMKEGIIATKIAAHAADLAK
CCCCHHHHHHHHCCHHEECCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
GVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREESKAKSKDSCTMCGKMCAVRN
HCCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
MNRVTEGKDLNMLRDDD
CHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMKLVSKGKVAIPANKNHKSLDPEGIGQ
CCCCCCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHCC
GLRTKINVNLGISKDCYNIDMELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAI
CCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
IGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFLTIHVGMNRKTAATFKKNPRR
EECCHHHHHHCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCE
MNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGAIAATHGADFLCYVTPAEHLRLPNLDDMKEGIIATKIAAHAADLAK
CCCCHHHHHHHHCCHHEECCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
GVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREESKAKSKDSCTMCGKMCAVRN
HCCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
MNRVTEGKDLNMLRDDD
CHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA