| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
Click here to switch to the map view.
The map label for this gene is mtbP [H]
Identifier: 170757755
GI number: 170757755
Start: 2334618
End: 2336303
Strand: Reverse
Name: mtbP [H]
Synonym: CLD_2435
Alternate gene names: 170757755
Gene position: 2336303-2334618 (Counterclockwise)
Preceding gene: 170754282
Following gene: 170756489
Centisome position: 59.02
GC content: 30.25
Gene sequence:
>1686_bases ATGTTAAAAGTAAGAACATTATTTAGTGGAATAGGTTCACCTGAAAGAGCTTTAAAAGATTTACAAATACCATATGAGTT AGTTGATTTTTGTGAAGTAGACAAATATGCAGTGAAAAGTTACTGTTCAGTTCATGGAGTTTCAGAGGAAAAGAATCTAG GTGATATTACAAAAGTTTGGGGAAGAAACCTTCCTTATGCAGATTTATTAGTTTGGGGGTTCCCATGCCCAGACATTAGT GTAGCAGGTAAACAGAAAGGAATAAAAGAAGGAGAAACTAGGAGTGGATTATATTACGAAGGATTTAGGATATTAAAAGA AACTAGACCTAAATATTCAATTATAGAAAATGTTAAAAACTTAGTAGGAAAGAGGTTTAAAGCTGATTTTGAAAGTATGC TTGAGGATATAGAAAGTTTAGGATATAACAACTATTGGAAAGTACTCAATGCTAAAGATTATGGGATACCTCAAAACAGA GAACGAGTGTTTATAGTGAGTATTAGAAAAGATATTGATACTGGGATCTTCAGTTTCCCTAAAGGATTTGACAATGGATT AAGACTTAAAGACTTTTTAGAGAATGAAGTTGATGAAAAGTATTATATAAGTGAAAAAGCCACTCAAGGGCTATTAAGGG CATTAAAAAGACCACATACACCTAAAATGCTTAATAAAGATTCGGAGTATTCACCAACATTAGATACAAGAGTTGGAGCG TTAACACATAGAAGTCCTTATATGTGCATTCCATGCATAACACCAGATAGGGCGGAAAAGCGTCAGAATGGCAGAAGATT TAAAGAAAATGGAGATCCTATGTTTACTCTAACAAGTCAAGATAGACATGGAATCTTACAAGTTGGAAACATAGTTGACA CAGGTAATTGGGGTAATCCGCAAAGAGGTAGAATTTATTCTCCGGATGGAATAAGTCCAGCACTAAATACCGTTGGTGGC GGAGGATTAGAACCTAAGATATTGAAATACAAAAGAACTGAGTACAGAATTCGCAAATTAACTCCAAAAGAATGTTGGAG ATTGATGGGTTTTACAGATGAAGATTTTCAAAAGGCTAAAGATGAAATTAGACCAATACTCAATGGCATTTGTATAAATA AAAATGAGTTTATAGCCTTAGATGGATATAGATTAGCACTAAGAAAAGGAAATTTTGAAACTAAAGAACCGGTTATTGTA GCAGCAGATTTAATAAAAGTAATTAACAAAGTTAAATATGAATGTAATGTTAAAATTTACTACAATGATAATTATGTTAA ATTTAAGTTTGACGATTTGGAAATAGTTGGTTATAGACGACAAGGCAATTATATAAATTATAATTCTATAATTCCAGAGA ATTATAACACTAAAGTTACATTAAAAATTAAACCTATACTTGATATTTTAAAAGATTATAAAAAGAATAAGTTTAAAGTT GTAGATTTAGATTTTCAAAATGACAAGTTAATTATTAAAGCTAATAACGATATTGCAACAGTAGAAGAAAGTATAACTAT AGAGTTACAAGGTGAACCTTTAGAAATTTCATTTAATGCAGATTATTTAATAGATGCATTTAAGAATTATGACAATGCAA CTTTAGAATTAACATATTGTGTAAATCCAATGGTAATAAGAGAAAATAATAAATTAGATTTAGTGCTACCAGTTAGAAAG AAATAG
Upstream 100 bases:
>100_bases TAATTTGAAATTATTGTGTACTAACTGAGATTAAAAAATAAATAGGAGTAATTCTAGATAAAAAGTGTGGCTACACCTCC TGACGAGGAGGAAGTATTAA
Downstream 100 bases:
>100_bases GGAGCTTAAAAGGCAGGTGAGAAACAGTGTTTAAAGGATACATTCCCACAGGTGGTAAAGATGGTAAAAGACCAACAGAA GAATATAAAGATAGAACAGA
Product: C-5 cytosine-specific DNA methylase
Products: NA
Alternate protein names: M.Phi3TI; Cytosine-specific methyltransferase Phi3TI [H]
Number of amino acids: Translated: 561; Mature: 561
Protein sequence:
>561_residues MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVWGRNLPYADLLVWGFPCPDIS VAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKNLVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNR ERVFIVSIRKDIDTGIFSFPKGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNPQRGRIYSPDGISPALNTVGG GGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAKDEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIV AADLIKVINKVKYECNVKIYYNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYCVNPMVIRENNKLDLVLPVRK K
Sequences:
>Translated_561_residues MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVWGRNLPYADLLVWGFPCPDIS VAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKNLVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNR ERVFIVSIRKDIDTGIFSFPKGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNPQRGRIYSPDGISPALNTVGG GGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAKDEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIV AADLIKVINKVKYECNVKIYYNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYCVNPMVIRENNKLDLVLPVRK K >Mature_561_residues MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVWGRNLPYADLLVWGFPCPDIS VAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKNLVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNR ERVFIVSIRKDIDTGIFSFPKGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNPQRGRIYSPDGISPALNTVGG GGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAKDEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIV AADLIKVINKVKYECNVKIYYNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYCVNPMVIRENNKLDLVLPVRK K
Specific function: This enzyme methylates the first cytosine within the sequences GGCC and GCNGC [H]
COG id: COG0270
COG function: function code L; Site-specific DNA methylase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the C5-methyltransferase family [H]
Homologues:
Organism=Escherichia coli, GI1788271, Length=208, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=5e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR018117 - InterPro: IPR001525 [H]
Pfam domain/function: PF00145 DNA_methylase [H]
EC number: =2.1.1.37 [H]
Molecular weight: Translated: 64656; Mature: 64656
Theoretical pI: Translated: 9.03; Mature: 9.03
Prosite motif: PS00094 C5_MTASE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVW CEEEEEHHHCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHH GRNLPYADLLVWGFPCPDISVAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKN CCCCCHHHEEEECCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH LVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNRERVFIVSIRKDIDTGIFSFP HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHCCC KGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA CCCCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHH LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNP EECCCCCEEEECCCCCHHHHHHCCCCHHCCCCEEEEEECCCCCCEEEECCEEECCCCCCC QRGRIYSPDGISPALNTVGGGGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAK CCCEEECCCCCCHHHHCCCCCCCCCHHEEEECCHHHEECCCHHHHHHHCCCCCHHHHHHH DEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIVAADLIKVINKVKYECNVKIY HHHHHHHCCEEECCCCEEEECCEEEEEECCCCCCCCCEEEHHHHHHHHHHCCEEEEEEEE YNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV ECCCEEEEEECCEEEEEEEECCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEE VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYC EEEECCCCEEEEEECCCEEEEEEEEEEEECCCEEEEEECCHHHHHHHHCCCCCEEEEEEE VNPMVIRENNKLDLVLPVRKK CCEEEEECCCCEEEEEECCCC >Mature Secondary Structure MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVW CEEEEEHHHCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHH GRNLPYADLLVWGFPCPDISVAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKN CCCCCHHHEEEECCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH LVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNRERVFIVSIRKDIDTGIFSFP HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHCCC KGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA CCCCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHH LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNP EECCCCCEEEECCCCCHHHHHHCCCCHHCCCCEEEEEECCCCCCEEEECCEEECCCCCCC QRGRIYSPDGISPALNTVGGGGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAK CCCEEECCCCCCHHHHCCCCCCCCCHHEEEECCHHHEECCCHHHHHHHCCCCCHHHHHHH DEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIVAADLIKVINKVKYECNVKIY HHHHHHHCCEEECCCCEEEECCEEEEEECCCCCCCCCEEEHHHHHHHHHHCCEEEEEEEE YNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV ECCCEEEEEECCEEEEEEEECCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEE VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYC EEEECCCCEEEEEECCCEEEEEEEEEEEECCCEEEEEECCHHHHHHHHCCCCCEEEEEEE VNPMVIRENNKLDLVLPVRKK CCEEEEECCCCEEEEEECCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]