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The map label for this gene is purN

Identifier: 170757645

GI number: 170757645

Start: 3112184

End: 3112801

Strand: Reverse

Name: purN

Synonym: CLD_1666

Alternate gene names: 170757645

Gene position: 3112801-3112184 (Counterclockwise)

Preceding gene: 170756492

Following gene: 170754463

Centisome position: 78.64

GC content: 28.8

Gene sequence:

>618_bases
ATGTTTAAAATTGCAGTGCTTGTTTCAGGAGGAGGAAGCAATCTTCAATCTATAATAGATAAAATAGAAGAGAGATATAT
AAAAAATTGCAAGATAGAAATGGTTATAGGGGATAGACCTAATATTTATGGTATAGAAAGAGCAGAGAAAAAGGGGATTA
AAACTTTAACTCTAGATAGAAAAATATATAAAAACAATCTATCTAATAAAATATCTGAGTGTTTATATGGAAAAGTAGAT
TTAATAGTTTTAGCAGGATGGCTTTCTATATTAAATGAGGATTTAATAAATAAATTTGAAAATAGGATAATAAACATCCA
TCCTTCATTAATACCAAGCTTTTGCGGAGATGGAATGTATGGTATAAAAGTGCACCAAAAAGCATTAGAATACGGAGTAA
AAGTATCTGGGTGTACTGTGCATTTTGTAGATGAGGGTACAGATAGTGGACCTATAATAATACAAAAATCTGTACCAGTT
TTTGCAGAAGATACAGCAGAAATATTGCAAAAAAGAGTTTTAGAAAAAGAACATGAGGCATTACCAGAAGCTATAAAACT
TATAAGTGAAGGAAAAGTAAAATTACAAGGAAGAAAAGTTTTTATTAAAACATACTAA

Upstream 100 bases:

>100_bases
ATAATTAGTTTATAGGTTAGAGCTTTAAAATAATTAATTAGGGAATTAATTATTTATAGAATATACATGAAAAATTTTCT
TTAAATTAAAGGAGAATAAG

Downstream 100 bases:

>100_bases
ATTTATAATAATGAAAAAATTACTATTAATATAAATGAAACTAATAGAAAAATGTGTTTAGATTAGATTAAATTTATTGA
AATATAAAAAATTGTAGTTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MFKIAVLVSGGGSNLQSIIDKIEERYIKNCKIEMVIGDRPNIYGIERAEKKGIKTLTLDRKIYKNNLSNKISECLYGKVD
LIVLAGWLSILNEDLINKFENRIINIHPSLIPSFCGDGMYGIKVHQKALEYGVKVSGCTVHFVDEGTDSGPIIIQKSVPV
FAEDTAEILQKRVLEKEHEALPEAIKLISEGKVKLQGRKVFIKTY

Sequences:

>Translated_205_residues
MFKIAVLVSGGGSNLQSIIDKIEERYIKNCKIEMVIGDRPNIYGIERAEKKGIKTLTLDRKIYKNNLSNKISECLYGKVD
LIVLAGWLSILNEDLINKFENRIINIHPSLIPSFCGDGMYGIKVHQKALEYGVKVSGCTVHFVDEGTDSGPIIIQKSVPV
FAEDTAEILQKRVLEKEHEALPEAIKLISEGKVKLQGRKVFIKTY
>Mature_205_residues
MFKIAVLVSGGGSNLQSIIDKIEERYIKNCKIEMVIGDRPNIYGIERAEKKGIKTLTLDRKIYKNNLSNKISECLYGKVD
LIVLAGWLSILNEDLINKFENRIINIHPSLIPSFCGDGMYGIKVHQKALEYGVKVSGCTVHFVDEGTDSGPIIIQKSVPV
FAEDTAEILQKRVLEKEHEALPEAIKLISEGKVKLQGRKVFIKTY

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=206, Percent_Identity=40.2912621359223, Blast_Score=154, Evalue=4e-38,
Organism=Homo sapiens, GI209869995, Length=206, Percent_Identity=40.2912621359223, Blast_Score=154, Evalue=4e-38,
Organism=Homo sapiens, GI209869993, Length=206, Percent_Identity=40.2912621359223, Blast_Score=154, Evalue=4e-38,
Organism=Escherichia coli, GI1788846, Length=207, Percent_Identity=36.231884057971, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787483, Length=140, Percent_Identity=31.4285714285714, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=36.3636363636364, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320616, Length=208, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582400, Length=196, Percent_Identity=37.2448979591837, Blast_Score=145, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22990; Mature: 22990

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIAVLVSGGGSNLQSIIDKIEERYIKNCKIEMVIGDRPNIYGIERAEKKGIKTLTLDR
CEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEECCCHHHHCCCEEEEEHH
KIYKNNLSNKISECLYGKVDLIVLAGWLSILNEDLINKFENRIINIHPSLIPSFCGDGMY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCE
GIKVHQKALEYGVKVSGCTVHFVDEGTDSGPIIIQKSVPVFAEDTAEILQKRVLEKEHEA
EHHHHHHHHHHCCEECCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
LPEAIKLISEGKVKLQGRKVFIKTY
HHHHHHHHHCCCEEEECEEEEEEEC
>Mature Secondary Structure
MFKIAVLVSGGGSNLQSIIDKIEERYIKNCKIEMVIGDRPNIYGIERAEKKGIKTLTLDR
CEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEECCCHHHHCCCEEEEEHH
KIYKNNLSNKISECLYGKVDLIVLAGWLSILNEDLINKFENRIINIHPSLIPSFCGDGMY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCE
GIKVHQKALEYGVKVSGCTVHFVDEGTDSGPIIIQKSVPVFAEDTAEILQKRVLEKEHEA
EHHHHHHHHHHCCEECCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
LPEAIKLISEGKVKLQGRKVFIKTY
HHHHHHHHHCCCEEEECEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]