| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is hupL
Identifier: 170757099
GI number: 170757099
Start: 1961580
End: 1962995
Strand: Reverse
Name: hupL
Synonym: CLD_2800
Alternate gene names: 170757099
Gene position: 1962995-1961580 (Counterclockwise)
Preceding gene: 170754415
Following gene: 170756705
Centisome position: 49.59
GC content: 29.8
Gene sequence:
>1416_bases ATGGGGAAGATTATTACTATTGATCCTATAACTAGGATTAGTGGATTTTTAGAAATAAAGGTAATAGTAGAAGACAATAT TATTATAGATGGTGAAACCAGCGGATTATTATTTAGAGGCTTTGAAAAAATGTTAGAAGGAAGACCTCCTTTAGATTCTA TATATTTTACTGAAAGAATATGTGGTATATGTTCTACTGCCCATGCTACAGCATCTACTTTAGCTTTAGAAAATGCATTA AATATAACTCCAAGTATAAATGATATATATATAAGAGATATGATGCATGGATTTGAATTTATACAAAACCACCTAAGGCA GTTTTATTGTTTTACTGTGCCAAGCTATGTAAATCTCCCTAATATAAGACCCATATACCCTCAGGAATATAGTGATTTTA GATTGCCTAAAAAGCTAAATGAAAGAATAAGTTCTCATTATGAAGAGGGCATAAAGTATAGTAGGTTAGCCCATGAAGCC TTAGCTGTGTTGGGAGGTAAAGCGCCTCATAGTCATGGTATTTTCGTAGGAGGGGTTACTGTAAATATAGATTCTTACAA ATTAACAAAAATACAATCCATATTAAAGACAATAAAAAAATTTGTAAATATCTATATGTTAGAGGATATGAATATAATTG CTCAGTATTACCCTGAATATTTTAATCTTGGAAACACTTATGGAAATTTTATGAGTTTTGGTGTATTTGATAAATATAAG GATAAAAATATAAGCTATGTAAAACCGGGAGTAATAATAAATAATAAAGTAGAAAATTTTGATAGAGATAAAATAACAGA AAACATTCGCTATGCTTGGTATGTAAGCGATACTATAATAGAAAATCCCATTGTGAACAATGAATATACTGTAGATTTAA AAAAAGAAGGAGCTTATAGTTTCGTAAAAGCACCTAGATATGATGGTTTACCTATGGAAGTAGGTCCTTTAGCTCGATTA AAAATTGCAGGAGAATATACCGGAGGCAATTCCTCTATGGATAGAAATATAGCCCGTGTTCTAGAAACTAAAAAAATAAT AAATATATTATATAATATATCAGAAAAAATTCAATTACTATCTAATAATCAATCTGCATATAATATTCCAGAAAGCGCCT TTGGAGAAGGTCTTATAGATACTACTAGGGGAGCCTTATCACACTTTATAAAAATAGAGAATAAAACTATAAAGTACTAT AATATTATAACTCCTACAGCTTGGAATATATCTCCTAAGGATTCAAAGGGTACAAGAGGTGTAGGAGAAAAATCCTTAAT AGGCTCAAAAATAGATAATATAGAAAAACCCGTAGAAATAGGAAGAATAATTCGTTCTTTTGATCCTTGCATTTCCTGTG CCACCCATTTAATAAGTAATAATAATGAACCAGTTAAAGTTGAAGTATTAGTATAG
Upstream 100 bases:
>100_bases TTCTATAGTTAAAAAAATAAAGTATATTAATTTTTTAGTTTAAATTATTTATGCTTAAGATAACTAAGTAAATTTTGAAT TATAAAAAGGGGAGGTCATT
Downstream 100 bases:
>100_bases CTATTAAAGTTATTGCTATAGGAAATATTTTAATGGGTGATGATGGTATAGCCATAAAAATAGCTAAAAATATTGAGGGA TTTTTAAAAGAAAATTATAT
Product: [Ni/Fe] hydrogenase, large subunit
Products: NA
Alternate protein names: NiFeSe hydrogenlyase large chain [H]
Number of amino acids: Translated: 471; Mature: 470
Protein sequence:
>471_residues MGKIITIDPITRISGFLEIKVIVEDNIIIDGETSGLLFRGFEKMLEGRPPLDSIYFTERICGICSTAHATASTLALENAL NITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLPNIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEA LAVLGGKAPHSHGIFVGGVTVNIDSYKLTKIQSILKTIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYK DKNISYVKPGVIINNKVENFDRDKITENIRYAWYVSDTIIENPIVNNEYTVDLKKEGAYSFVKAPRYDGLPMEVGPLARL KIAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLLSNNQSAYNIPESAFGEGLIDTTRGALSHFIKIENKTIKYY NIITPTAWNISPKDSKGTRGVGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLISNNNEPVKVEVLV
Sequences:
>Translated_471_residues MGKIITIDPITRISGFLEIKVIVEDNIIIDGETSGLLFRGFEKMLEGRPPLDSIYFTERICGICSTAHATASTLALENAL NITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLPNIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEA LAVLGGKAPHSHGIFVGGVTVNIDSYKLTKIQSILKTIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYK DKNISYVKPGVIINNKVENFDRDKITENIRYAWYVSDTIIENPIVNNEYTVDLKKEGAYSFVKAPRYDGLPMEVGPLARL KIAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLLSNNQSAYNIPESAFGEGLIDTTRGALSHFIKIENKTIKYY NIITPTAWNISPKDSKGTRGVGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLISNNNEPVKVEVLV >Mature_470_residues GKIITIDPITRISGFLEIKVIVEDNIIIDGETSGLLFRGFEKMLEGRPPLDSIYFTERICGICSTAHATASTLALENALN ITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLPNIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEAL AVLGGKAPHSHGIFVGGVTVNIDSYKLTKIQSILKTIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYKD KNISYVKPGVIINNKVENFDRDKITENIRYAWYVSDTIIENPIVNNEYTVDLKKEGAYSFVKAPRYDGLPMEVGPLARLK IAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLLSNNQSAYNIPESAFGEGLIDTTRGALSHFIKIENKTIKYYN IITPTAWNISPKDSKGTRGVGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLISNNNEPVKVEVLV
Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]
COG id: COG0374
COG function: function code C; Ni,Fe-hydrogenase I large subunit
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]
Homologues:
Organism=Escherichia coli, GI1789368, Length=574, Percent_Identity=28.2229965156794, Blast_Score=230, Evalue=1e-61, Organism=Escherichia coli, GI1787207, Length=583, Percent_Identity=28.3018867924528, Blast_Score=222, Evalue=4e-59,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001501 - InterPro: IPR018194 [H]
Pfam domain/function: PF00374 NiFeSe_Hases [H]
EC number: =1.12.99.6 [H]
Molecular weight: Translated: 53314; Mature: 53183
Theoretical pI: Translated: 7.89; Mature: 7.89
Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGKIITIDPITRISGFLEIKVIVEDNIIIDGETSGLLFRGFEKMLEGRPPLDSIYFTERI CCCEEEECCHHHCCCEEEEEEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHH CGICSTAHATASTLALENALNITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLP HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC NIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEALAVLGGKAPHSHGIFVGGVT CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEE VNIDSYKLTKIQSILKTIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYK EEECCCHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC DKNISYVKPGVIINNKVENFDRDKITENIRYAWYVSDTIIENPIVNNEYTVDLKKEGAYS CCCCCEECCCEEECCCCCCCCHHHHHCCCEEEEEEECHHHCCCCCCCEEEEEEECCCCCH FVKAPRYDGLPMEVGPLARLKIAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLL HHCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH SNNQSAYNIPESAFGEGLIDTTRGALSHFIKIENKTIKYYNIITPTAWNISPKDSKGTRG CCCCCCCCCCHHHHCCCCHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCCCCCCCC VGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLISNNNEPVKVEVLV CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC >Mature Secondary Structure GKIITIDPITRISGFLEIKVIVEDNIIIDGETSGLLFRGFEKMLEGRPPLDSIYFTERI CCEEEECCHHHCCCEEEEEEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHH CGICSTAHATASTLALENALNITPSINDIYIRDMMHGFEFIQNHLRQFYCFTVPSYVNLP HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC NIRPIYPQEYSDFRLPKKLNERISSHYEEGIKYSRLAHEALAVLGGKAPHSHGIFVGGVT CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEE VNIDSYKLTKIQSILKTIKKFVNIYMLEDMNIIAQYYPEYFNLGNTYGNFMSFGVFDKYK EEECCCHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC DKNISYVKPGVIINNKVENFDRDKITENIRYAWYVSDTIIENPIVNNEYTVDLKKEGAYS CCCCCEECCCEEECCCCCCCCHHHHHCCCEEEEEEECHHHCCCCCCCEEEEEEECCCCCH FVKAPRYDGLPMEVGPLARLKIAGEYTGGNSSMDRNIARVLETKKIINILYNISEKIQLL HHCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH SNNQSAYNIPESAFGEGLIDTTRGALSHFIKIENKTIKYYNIITPTAWNISPKDSKGTRG CCCCCCCCCCHHHHCCCCHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCCCCCCCC VGEKSLIGSKIDNIEKPVEIGRIIRSFDPCISCATHLISNNNEPVKVEVLV CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3316183; 10378275 [H]