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The map label for this gene is punA

Identifier: 170756883

GI number: 170756883

Start: 1987718

End: 1988533

Strand: Reverse

Name: punA

Synonym: CLD_2776

Alternate gene names: 170756883

Gene position: 1988533-1987718 (Counterclockwise)

Preceding gene: 170757059

Following gene: 170754547

Centisome position: 50.24

GC content: 31.25

Gene sequence:

>816_bases
TTGAATTTAAAGAAAATAAAAGAAAGTGCAGATTTTGTGAAAAATAACATATCTATAATGCCTGAAATTGGAATTATTTT
AGGCTCAGGCTTAGGTGATTTAGCTGAGGAAATAGAAAATAAAGAAATTATAAAATATTCCAATATACCTAATATGCCAG
CTTCAACTATAAAAGGACATAAGGGTCAGTTTGTAGTGGGAACTTTAAAGGGCAAAAAGGTTATAATGATGCAAGGGAGA
GTGCATTATTATGAAGGAAATAAAATGAGCGATGTAGTACTTCCTGTATACATAATGAAAGAGCTAGGAGTAAAAAATAT
AATTGTTACAAATGCAGCTGGGGGAGTAAATGAAATTTATAATCCTGGAGATCTTATGATTATAAAAGATCATATTAATT
TTGCTTTTAGTAATCCTTTAATAGGTAGTAATGATGAGGAAATTGGACCAAGATTTCCAGATATGTCAGAAGCATATAAT
AAAAGTTTGATAGTCCTTGCAGAAAAAGAAGCTGAAAAATTAGGCTTTTCTCTTCAAAAGGGAGTATATGCTATGATGAC
AGGACCTTCATATGAAACACCAGCAGAAATAAAAATGCTAAGAATGTTAGGGGCAGATGCAGTAGGAATGTCTACAGTAC
CAGAGGTTATAGCAGCAAATCATTGTGGAATTAAAGTATTGGGAATATCTTGTATAACCAATATGGCAGCAGGTATATTA
GAGCAGCCACTAAACCATCAAGAAGTTATAGAAACATCTAATAAAGTAAGATCTAAATTCATATCCTTACTAAAATCAAT
ATTAGAAGTTATGTAA

Upstream 100 bases:

>100_bases
TCTAGTATATCAAAGAAGAGTAAAATAGCAGAAGTATATAAAAATGCTCATCCAAGGGCATAATTTTAATAAAGCTTTCA
AGATAAAGGAGGAGTTAATT

Downstream 100 bases:

>100_bases
AATTTTTAATAATAAAAAATGGGTTGATAAGTGATTCTTAGGGTCAAACATAGAAATGTTTTGTATAAACAAGCTCTACT
TGCTCCTAAGAATCACTTAA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNLKKIKESADFVKNNISIMPEIGIILGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGHKGQFVVGTLKGKKVIMMQGR
VHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIYNPGDLMIIKDHINFAFSNPLIGSNDEEIGPRFPDMSEAYN
KSLIVLAEKEAEKLGFSLQKGVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
EQPLNHQEVIETSNKVRSKFISLLKSILEVM

Sequences:

>Translated_271_residues
MNLKKIKESADFVKNNISIMPEIGIILGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGHKGQFVVGTLKGKKVIMMQGR
VHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIYNPGDLMIIKDHINFAFSNPLIGSNDEEIGPRFPDMSEAYN
KSLIVLAEKEAEKLGFSLQKGVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
EQPLNHQEVIETSNKVRSKFISLLKSILEVM
>Mature_271_residues
MNLKKIKESADFVKNNISIMPEIGIILGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGHKGQFVVGTLKGKKVIMMQGR
VHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIYNPGDLMIIKDHINFAFSNPLIGSNDEEIGPRFPDMSEAYN
KSLIVLAEKEAEKLGFSLQKGVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
EQPLNHQEVIETSNKVRSKFISLLKSILEVM

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=269, Percent_Identity=46.0966542750929, Blast_Score=244, Evalue=5e-65,
Organism=Homo sapiens, GI47132622, Length=259, Percent_Identity=30.1158301158301, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788746, Length=249, Percent_Identity=41.7670682730924, Blast_Score=217, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17541190, Length=250, Percent_Identity=44.4, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323238, Length=271, Percent_Identity=40.590405904059, Blast_Score=199, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24656093, Length=273, Percent_Identity=45.4212454212454, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI45552885, Length=273, Percent_Identity=45.4212454212454, Blast_Score=240, Evalue=9e-64,
Organism=Drosophila melanogaster, GI45552887, Length=273, Percent_Identity=45.4212454212454, Blast_Score=240, Evalue=9e-64,
Organism=Drosophila melanogaster, GI24656090, Length=273, Percent_Identity=45.4212454212454, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24762376, Length=240, Percent_Identity=36.25, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI221459247, Length=159, Percent_Identity=30.8176100628931, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20130079, Length=249, Percent_Identity=24.8995983935743, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29602; Mature: 29602

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKKIKESADFVKNNISIMPEIGIILGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGH
CCHHHHHHHHHHHHCCCEECCCCCEEEECCHHHHHHHHCCCCEEEECCCCCCCHHHCCCC
KGQFVVGTLKGKKVIMMQGRVHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIY
CCCEEEEEECCCEEEEEECEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCC
NPGDLMIIKDHINFAFSNPLIGSNDEEIGPRFPDMSEAYNKSLIVLAEKEAEKLGFSLQK
CCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCHHHHCCHHHC
GVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
CEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
EQPLNHQEVIETSNKVRSKFISLLKSILEVM
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNLKKIKESADFVKNNISIMPEIGIILGSGLGDLAEEIENKEIIKYSNIPNMPASTIKGH
CCHHHHHHHHHHHHCCCEECCCCCEEEECCHHHHHHHHCCCCEEEECCCCCCCHHHCCCC
KGQFVVGTLKGKKVIMMQGRVHYYEGNKMSDVVLPVYIMKELGVKNIIVTNAAGGVNEIY
CCCEEEEEECCCEEEEEECEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCC
NPGDLMIIKDHINFAFSNPLIGSNDEEIGPRFPDMSEAYNKSLIVLAEKEAEKLGFSLQK
CCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCHHHHCCHHHC
GVYAMMTGPSYETPAEIKMLRMLGADAVGMSTVPEVIAANHCGIKVLGISCITNMAAGIL
CEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
EQPLNHQEVIETSNKVRSKFISLLKSILEVM
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]