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The map label for this gene is pfl

Identifier: 170756881

GI number: 170756881

Start: 3492652

End: 3494880

Strand: Reverse

Name: pfl

Synonym: CLD_1316

Alternate gene names: 170756881

Gene position: 3494880-3492652 (Counterclockwise)

Preceding gene: 170757732

Following gene: 170755341

Centisome position: 88.29

GC content: 29.97

Gene sequence:

>2229_bases
ATGTTTTATGATTCATGGAAAAAATTTAATGAAGGTTCATGGGAAAATAATATAAATGTAAGAGAGTTTATACAAAATAA
TTATACGCCTTATTATGGAGATCATAGCTTTTTAAAGGAATCTACTGAAAAAACGAAAGATCTTTGGAAACAATGCGAAA
CATTAATAGCAGAAGAAATAAAAAAAGGAATATTAGATGTAGATCTAGATAATATTTCTGCTATAAATGCTTTTGATGCA
GGATATATAGACAAGGATAATGAAACCATAGTAGGTCTTCAGACAGATAAACCACTAAAAAGAATTATAAATCCTTTTGG
TGGTATAAGAATGGTAAAACAAGCTTTAGAAGCCTATGATTATAAGTTAAACCCAGACATAAAAGATATATTTACCAAAT
ATAGAAAAACCCATAATGATGGTGTTTTTGATGCTTATACTGAAGAGATGAGAAAAGCCAGAAGTGCAGGATTATTAACA
GGGCTTCCAGATGCCTATGGAAGAGGGAGAATAATAGGTGATTATAGAAGAATACCATTATATGGGGTAGATTTTTTAAT
AAAAAATAAAGAAGAAGATTTAAAAGCTGTTAAAGGGGAAATGAATGAAACTACCATAAGAAAAAGAGAAGAGATAAGCG
AACAAATAAAAGCTTTAATAGCTATGAAAGAAATGGCACTAAAGTATGGAATAGACATAAGCAAGCCAGCTAAAAATGCA
GAAGAAGCAGTACAATTTTTATATTTTGGTTATTTAGCAGGAGTTAAAGAAAATAATGGGGCGGCGATGTCCTTAGGAAG
AGTAAGTTCATTTATAGATATATATATAGAAAGAGACTTAAAACAGGGAATACTAACAGAAGAAAAAGCGCAAGAAATTA
TAGATCAGTTTGTTATAAAATTAAGATTAGTAAGACATCTTAGAACACCAGAGTATAATGATCTTTTTGCAGGAGATCCT
AATTGGATTACAGAAGCTATAGGAGGAATGGGATTAAATGGAGAAACTTTAGTTACTAAAACATCCTATAGATTTTTAAA
TACTTTAAATAATTTAGGACCAGCACCAGAACCTAATATGACAGTATTATGGTCACAAAACTTGCCAGAAAACTTTAAAA
AATTCTGTGCAGAAATGTCTATAAAGACAGATTCTATTCAATATGAAAATGACGATTTAATGAGAGATATATATGGAGAT
GATTATGGTATAGCTTGTTGTGTATCAGCTATGGCCTTGGGAAAACAGATGCAATTTTTCGGTGCAAGATGCAATTTAGC
CAAAGCTTTATTGTATTCCATAAATGGTGGAGTAGATGAAAAGAAGAATATTAAAGTAATAGATAATATAAATGCAATAG
AAGATACTGTGTTAGATTATGAAAAAGTTAAGGAAAATTATTTTAAAGTATTAGAATATATAGCAGATTTATATGTAAAT
ACTATGAATATAATACATTATATGCATGATAAATATGCTTATGAAGGTGGGCTTATGGCACTTCATGATACAGAAGTAGA
AAGACTTATGGCCTTTGGTGTAGCTGGATTGTCTGTTGTAGCAGATTCATTAAGTGCTATAAAATATGCAAAAGTGAAAC
CAATAAGAGAAAATGGTATTGCTGTAGATTTTGAAATAGAAGGGGATTTCCCTAAATACGGTAATGATGATGATAGAGCT
GATGAAATAGCAGTGGAGATAGTTAATAAATTTATTAATGAATTAAAGAAAAATAAAACTTATAGAGATGCAAAACATAC
ACTTTCAGTTTTAACAATAACTTCTAATGTAGTGTATGGTAAGAAAACAGGTTCAACACCAGATGGAAGAAAATCGGGAG
AAGCATTTGCTCCAGGCGCTAATCCTATGCATGGAAGAGATAAAAATGGTGCTTTAGCATCATTAAATTCTGTAGCTAAA
ATACCTTACAAAAATGTTTGTGAAGATGGAGTATCAAATACATTTTCTATAGTTCCAGATGCATTGGGTAAAAGTGAAGA
AGAAAGAATAAATAATTTAGTATCTATATTAGATGGATATTTTGTACAAAATGCTCATCATTTAAATGTTAATGTGTTAA
ATAGAGATCTATTAATAGATGCAATGGAACATCCAGAAAAATATCCATCACTTACTATAAGGGTATCAGGTTATGCAGTT
CATTTTAATAGATTGACTAAAGCACAACAATTAGAAGTGATAAGCAGAACTTTCCATAAAGACATGTAA

Upstream 100 bases:

>100_bases
TAGGAGTAAATTGTGCTTTGCAAATTAAAGTTAGACTTGGTTTTTTAGGCAATATAAGCTCTAAATGCTTTTAAATATAT
TAAATTTTAGGAGGTTTGCT

Downstream 100 bases:

>100_bases
ATTATTTCCATATTATAATTATTGGGATTAATGTTTCTAGTCTTTTTAGATGAGATATATTATAGGTTCTTATGCTGTAT
ATAAGGGGTGTTATAGCCCT

Product: formate acetyltransferase 1

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEIKKGILDVDLDNISAINAFDA
GYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYDYKLNPDIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLT
GLPDAYGRGRIIGDYRRIPLYGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEEKAQEIIDQFVIKLRLVRHLRTPEYNDLFAGDP
NWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNMTVLWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGD
DYGIACCVSAMALGKQMQFFGARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTVLDYEKVKENYFKVLEYIADLYVN
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPIRENGIAVDFEIEGDFPKYGNDDDRA
DEIAVEIVNKFINELKKNKTYRDAKHTLSVLTITSNVVYGKKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAK
IPYKNVCEDGVSNTFSIVPDALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HFNRLTKAQQLEVISRTFHKDM

Sequences:

>Translated_742_residues
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEIKKGILDVDLDNISAINAFDA
GYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYDYKLNPDIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLT
GLPDAYGRGRIIGDYRRIPLYGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEEKAQEIIDQFVIKLRLVRHLRTPEYNDLFAGDP
NWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNMTVLWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGD
DYGIACCVSAMALGKQMQFFGARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTVLDYEKVKENYFKVLEYIADLYVN
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPIRENGIAVDFEIEGDFPKYGNDDDRA
DEIAVEIVNKFINELKKNKTYRDAKHTLSVLTITSNVVYGKKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAK
IPYKNVCEDGVSNTFSIVPDALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HFNRLTKAQQLEVISRTFHKDM
>Mature_742_residues
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEIKKGILDVDLDNISAINAFDA
GYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYDYKLNPDIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLT
GLPDAYGRGRIIGDYRRIPLYGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEEKAQEIIDQFVIKLRLVRHLRTPEYNDLFAGDP
NWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNMTVLWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGD
DYGIACCVSAMALGKQMQFFGARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTVLDYEKVKENYFKVLEYIADLYVN
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPIRENGIAVDFEIEGDFPKYGNDDDRA
DEIAVEIVNKFINELKKNKTYRDAKHTLSVLTITSNVVYGKKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAK
IPYKNVCEDGVSNTFSIVPDALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HFNRLTKAQQLEVISRTFHKDM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=751, Percent_Identity=60.9853528628495, Blast_Score=955, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=751, Percent_Identity=59.9201065246338, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=665, Percent_Identity=26.1654135338346, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1790388, Length=730, Percent_Identity=24.2465753424658, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788933, Length=61, Percent_Identity=63.9344262295082, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 83962; Mature: 83962

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEI
CCCCCHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKGILDVDLDNISAINAFDAGYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYD
HHCCEECCCCCCCEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHC
YKLNPDIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLTGLPDAYGRGRIIGDYRRIPL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEECCE
YGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
ECCHHEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEEKAQEIIDQFVIK
HHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LRLVRHLRTPEYNDLFAGDPNWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNM
HHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCE
TVLWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGDDYGIACCVSAMALGKQMQFF
EEEECCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTVLDYEKVKENYFKVLEYIADLYVN
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPIRENGI
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AVDFEIEGDFPKYGNDDDRADEIAVEIVNKFINELKKNKTYRDAKHTLSVLTITSNVVYG
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECCEEEC
KKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAKIPYKNVCEDGVSNTFSIVPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCHHHHCCH
ALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCCCCEEEEEEEEEE
HFNRLTKAQQLEVISRTFHKDM
EHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEI
CCCCCHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKGILDVDLDNISAINAFDAGYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYD
HHCCEECCCCCCCEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHC
YKLNPDIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLTGLPDAYGRGRIIGDYRRIPL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEECCE
YGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
ECCHHEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEEKAQEIIDQFVIK
HHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LRLVRHLRTPEYNDLFAGDPNWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNM
HHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCE
TVLWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGDDYGIACCVSAMALGKQMQFF
EEEECCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTVLDYEKVKENYFKVLEYIADLYVN
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPIRENGI
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AVDFEIEGDFPKYGNDDDRADEIAVEIVNKFINELKKNKTYRDAKHTLSVLTITSNVVYG
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECCEEEC
KKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAKIPYKNVCEDGVSNTFSIVPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCHHHHCCH
ALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCCCCEEEEEEEEEE
HFNRLTKAQQLEVISRTFHKDM
EHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]