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The map label for this gene is miaA

Identifier: 170756578

GI number: 170756578

Start: 1913761

End: 1914696

Strand: Reverse

Name: miaA

Synonym: CLD_2842

Alternate gene names: 170756578

Gene position: 1914696-1913761 (Counterclockwise)

Preceding gene: 170757624

Following gene: 170755955

Centisome position: 48.37

GC content: 28.53

Gene sequence:

>936_bases
ATGATAGATTTATTAATAATTGCAGGTCCTACAGCAGTAGGGAAAACAGATATTTCCATAAAACTTGCAGAAAAACTAAA
TGGAGAGATAATATCCGCTGACTCTATGCAAATTTATAAATACATGGATATAGGTTCAGCCAAAATAACTAAGGATGAAA
TGAAAGGGATACCTCACCATCTTATAGATGTAGTAGAACCCCATGAAGAATTCAATGTATCTTCCTTTAAAGATCTAGCA
GAGAAATCTATAAAAGATATATGGAACAGAGGAAAGTTGCCCATAATAGCAGGAGGTACAGGGTTATATATTAATTCTTT
AATATACAATTATGATTTTACAGATGCAGATAGAGACGAAAAATATAGAGAATATCTTACTAAATTAGCAGAAGATAAAG
GGAATGAATATGTTCATAGCCTGCTAAAAGATATAGATGAAGAATCCTATGAAAAGTTATATCCTAATGATCTAAAAAGA
GTAGTAAGAGCCTTAGAAGTTTACAAAATCACAGGTAAATCTATAAGCGAATACATAAAAGAAAACGAGAAAAAATTATA
TGATATCCCTTATAACGTAAACTACTTTATTTTAAATATGAATAGAGAGGTACTCTATGAAAGAATTAATAAAAGAGTGG
ATATAATGATGGGTAAAGGTTTAATAGAAGAAGTTAAAAAATTAGAGTCCATGGGATATACTCCAGATATGCAATCTATG
AAAGGTATAGGGTATAAAGAAGTTTTATTCTATTTAAATGGAGATATTTCACTAGATGAAGCTATTTATTTAATAAAAAA
AGGAAGCAGAAATTATGCTAAAAGACAACTTACTTGGTTTAGAAAAGATAAAAGATCTATATGGATAGATAAAGATAAAT
ATAGCTCTGAGGAGGAAATAGTAGATAAAATTATAAAAATGGTAAAAGATAAATAG

Upstream 100 bases:

>100_bases
TCCATATACTTGTCCCCATGGAAGACCTACTATGATTAAATTTACATTAAAAGATTTAGAAAAAAAATTTAAAAGAATAC
AATGAGGGGGATAAGGAAAA

Downstream 100 bases:

>100_bases
AATTAAAAGGATTTTTAATTTATTTATAGAATTATATAAGAATAAGTTTCTAATTATTTGTATTTTTTTAGGAGGGAAAG
GTTATATGACTAAAGTTGTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHHLIDVVEPHEEFNVSSFKDLA
EKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDEKYREYLTKLAEDKGNEYVHSLLKDIDEESYEKLYPNDLKR
VVRALEVYKITGKSISEYIKENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEIVDKIIKMVKDK

Sequences:

>Translated_311_residues
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHHLIDVVEPHEEFNVSSFKDLA
EKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDEKYREYLTKLAEDKGNEYVHSLLKDIDEESYEKLYPNDLKR
VVRALEVYKITGKSISEYIKENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEIVDKIIKMVKDK
>Mature_311_residues
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHHLIDVVEPHEEFNVSSFKDLA
EKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDEKYREYLTKLAEDKGNEYVHSLLKDIDEESYEKLYPNDLKR
VVRALEVYKITGKSISEYIKENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEIVDKIIKMVKDK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=32.0388349514563, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=34.9673202614379, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI25144712, Length=274, Percent_Identity=33.9416058394161, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=35.6164383561644, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24649830, Length=268, Percent_Identity=33.2089552238806, Blast_Score=164, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36245; Mature: 36245

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHH
CEEEEEEECCCCCCCCCEEEEEHHHHCCCEEECCCEEEEEEEECCCCEECHHHHHCCCHH
LIDVVEPHEEFNVSSFKDLAEKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDE
HHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEEHHHHEECCCCCCCCHH
KYREYLTKLAEDKGNEYVHSLLKDIDEESYEKLYPNDLKRVVRALEVYKITGKSISEYIK
HHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
ENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
CCCCEEEECCCCCEEEEEECCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEI
HCCCHHHEEEEECCCCCHHHHEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
VDKIIKMVKDK
HHHHHHHHCCC
>Mature Secondary Structure
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHH
CEEEEEEECCCCCCCCCEEEEEHHHHCCCEEECCCEEEEEEEECCCCEECHHHHHCCCHH
LIDVVEPHEEFNVSSFKDLAEKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDE
HHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEEHHHHEECCCCCCCCHH
KYREYLTKLAEDKGNEYVHSLLKDIDEESYEKLYPNDLKRVVRALEVYKITGKSISEYIK
HHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
ENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
CCCCEEEECCCCCEEEEEECCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEI
HCCCHHHEEEEECCCCCHHHHEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
VDKIIKMVKDK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA