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The map label for this gene is leuC2 [H]

Identifier: 170756378

GI number: 170756378

Start: 529951

End: 531876

Strand: Direct

Name: leuC2 [H]

Synonym: CLD_0325

Alternate gene names: 170756378

Gene position: 529951-531876 (Clockwise)

Preceding gene: 170757427

Following gene: 170755526

Centisome position: 13.39

GC content: 31.57

Gene sequence:

>1926_bases
TTGGGTTTAACATTAACAGAAAAGATAATAAAATCACATTTAGTAAAAGGAAATATGACTAAGGGAGAAGAAATTGCTAT
TAAAATAGATAATACTTTAACTCAGGATTCTACGGGAACTATGGCCTATCTTCAATTTGAAGCTTTAGGTATAGATAAAG
TAAAGACTAAAAGATCAGTGGCTTATATAGACCACAATATGTTGCAAAGTGGACCAGAGAATGCGGATGATCATTTATAT
ATACAAACAGTAGCTAAAAAACATGGAATTTATTTTTCAAAACCAGGTAATGGAATATGTCATCAAGTTAATTTGGAAAG
ATTTGGTGTACCTGGAGAAACTTTATTAGGATCCGATAGCCATACTCCAACAGGAGGAGGTATAGGAATGTTAGCTATAG
GTGCTGGTGGATTGGATGTAGCAGTAGCTATGGCAGGTGGAGAATATTATATAAATATGCCTAAAGTAGTTAAGGTAAAT
TTAATAGGAAAATTAAAACCTTTTGTATCTGCAAAAGATATTATCTTAGAAGTTTTAAGAAGATTATCTGTAAAGGGTGG
AGTAGGAAAAATATTTGAATATACAGGAGAAGGAGTAAAAGCTCTTACAGTTCCAGAGAGAGCAACTATAACTAATATGG
GAGCAGAACTTGGAGCTACTACATCAATCTTTCCATCAGATGAAATTACTAAAGATTTTTTAAAGGCTCAAGAAAGAGAA
GAAGATTATAAGTATTTAGTAGCAGATGAGGATGCTGTTTATGATGAAGAATTAGAAATAAATTTATCAGATCTAAAACC
TTTAGCTGCATGTCCACATAGTCCTGATAATATAGTACCTGTATCAGAACTTAAAAATATAAAAGTTAATCAAGTAGCTA
TAGGTAGCTGTACAAATTCATCTTATAGAGATATGTTAAGAACAGCCAGTATACTAAAAGGAAAGACTGTATCAGAAAAT
GTATCTTTAGTTATAGCCCCAGGTTCTAAGCAAGTTCTAAACATGATGGCTAATAATGGAGCTTTAACAGATTTAGTTAT
GGCAGGAGCAAGAATACTAGAATGTGCCTGTGGACCATGTATAGGAATGGGACAATCTCCTTGTACAGATGCTGTATCTT
TAAGAACTTTTAATAGAAATTTTGAGGGTAGATCTGGAACAAAATCTGCTAATGTGTATTTAGTAAGTCCAGAAGTGGCA
GCAGTTTCAGCATTAACAGGATATATAACAGACCCAACTGAGTTAACTGATTTTGAAGATATAGAAATGCCTAATAAATT
TTTGATAAATGATAATTTAATAATAGAACCAGCTACAGAAGGAGAAGTTGTAGAGGTTAAAAGAGGACCAAATATAAAAC
CTTTCCCTAAAGCAGAAGCTTTAAAAGAATTAGTAACTGGAAAGGCATTAACTAAAGTAGGGGACAATATAACAACAGAT
CATATAATGCCATCTAATGCTAAACTTTTACCTTATAGATCTAATGTACCATATTTATCAAACTTTTGCTTAACTCCTTG
TGATGAAGAATTTCCTGAAAAAGCTAAGGAAAATGGAGGAGGTATAATAGTCGCAGGAACAAATTACGGACAAGGTTCTA
GTAGAGAACATGCAGCCTTAGCTCCCGTTTATTTAGGAATAAAAGCAGTTTTAGCAAAATCCTTTGCTAGAATTCATAAA
GCAAATCTAATAAATAATGGTATATTACCTTTAGTTTTTGAAAGAGAAGAAGATTATGATTCTATAGATGAAATGGATGA
ATTAGTAATAGAAAATGTACTAAATCAAGTGGAAATAGGTAAAATAGATATAATAAATAAAACTAAGGGCATAAAATATA
AAACTTTATTAGAAGCAACAGATAGACAAAAGAAGATGATTTTAAAGGGCGGATTATTAAATTTAATAAAAAGTTCTCAA
CAATAG

Upstream 100 bases:

>100_bases
AAACCCTATAAATAAAAACTAAAGAGAGTATAAATAAAAAATTAGTAAGTAAAAAACAAAAGATAAATATTAAAGTATAA
ATTGATTGGGGGAATAACAG

Downstream 100 bases:

>100_bases
CTACAAAAATTTGGTTAAGTTTTCGGAGGGATATGATGAAACACAATATAACTTTAATACCAGGAGATGGTATAGGACCA
GAAGTAACTGAAGCTGCAAG

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MGLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSVAYIDHNMLQSGPENADDHLY
IQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVN
LIGKLKPFVSAKDIILEVLRRLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
EDYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNSSYRDMLRTASILKGKTVSEN
VSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPCIGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVA
AVSALTGYITDPTELTDFEDIEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAALAPVYLGIKAVLAKSFARIHK
ANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIGKIDIINKTKGIKYKTLLEATDRQKKMILKGGLLNLIKSSQ
Q

Sequences:

>Translated_641_residues
MGLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSVAYIDHNMLQSGPENADDHLY
IQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVN
LIGKLKPFVSAKDIILEVLRRLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
EDYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNSSYRDMLRTASILKGKTVSEN
VSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPCIGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVA
AVSALTGYITDPTELTDFEDIEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAALAPVYLGIKAVLAKSFARIHK
ANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIGKIDIINKTKGIKYKTLLEATDRQKKMILKGGLLNLIKSSQ
Q
>Mature_640_residues
GLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSVAYIDHNMLQSGPENADDHLYI
QTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVNL
IGKLKPFVSAKDIILEVLRRLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQEREE
DYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNSSYRDMLRTASILKGKTVSENV
SLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPCIGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVAA
VSALTGYITDPTELTDFEDIEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTDH
IMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAALAPVYLGIKAVLAKSFARIHKA
NLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIGKIDIINKTKGIKYKTLLEATDRQKKMILKGGLLNLIKSSQQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=722, Percent_Identity=35.0415512465374, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI8659555, Length=732, Percent_Identity=26.9125683060109, Blast_Score=192, Evalue=8e-49,
Organism=Homo sapiens, GI41352693, Length=660, Percent_Identity=27.4242424242424, Blast_Score=180, Evalue=5e-45,
Organism=Escherichia coli, GI87081781, Length=723, Percent_Identity=27.5242047026279, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=28.3261802575107, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1787531, Length=633, Percent_Identity=27.3301737756714, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI2367097, Length=461, Percent_Identity=25.8134490238612, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25149337, Length=715, Percent_Identity=35.5244755244755, Blast_Score=320, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI32564738, Length=679, Percent_Identity=35.9351988217968, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI25149342, Length=565, Percent_Identity=35.3982300884956, Blast_Score=255, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI17568399, Length=770, Percent_Identity=26.4935064935065, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6322261, Length=712, Percent_Identity=34.2696629213483, Blast_Score=345, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6323335, Length=728, Percent_Identity=33.2417582417582, Blast_Score=329, Evalue=9e-91,
Organism=Saccharomyces cerevisiae, GI6320440, Length=611, Percent_Identity=32.8968903436989, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321429, Length=670, Percent_Identity=27.3134328358209, Blast_Score=193, Evalue=6e-50,
Organism=Drosophila melanogaster, GI281365315, Length=724, Percent_Identity=35.4972375690608, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI17864292, Length=724, Percent_Identity=35.4972375690608, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI28571643, Length=720, Percent_Identity=34.1666666666667, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI161076999, Length=688, Percent_Identity=36.046511627907, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24645686, Length=759, Percent_Identity=26.0869565217391, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI17137564, Length=724, Percent_Identity=25.9668508287293, Blast_Score=167, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 69385; Mature: 69253

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSV
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCE
AYIDHNMLQSGPENADDHLYIQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDS
EEEEHHHHHCCCCCCCCEEEEEEEHHHCCEEEECCCCCCEEEECHHHCCCCCCCEECCCC
HTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVNLIGKLKPFVSAKDIILEVLR
CCCCCCCEEEEEEECCCCEEEEEEECCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHH
RLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
HHCCCCCCCHHHHCCCCCEEEEECCCCHHHHHCCHHHCCCEECCCCHHHHHHHHHHHHCC
EDYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNS
CCCEEEEECCCCCCCCEEEEEHHHCCHHHCCCCCCCCEEEHHHHCCCEEEEEEEECCCCH
SYRDMLRTASILKGKTVSENVSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPC
HHHHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
IGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVAAVSALTGYITDPTELTDFED
CCCCCCCCCCHHEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHH
IEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
CCCCCEEEECCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCC
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAAL
CCCCCCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCH
APVYLGIKAVLAKSFARIHKANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
KIDIINKTKGIKYKTLLEATDRQKKMILKGGLLNLIKSSQQ
EEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSV
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCE
AYIDHNMLQSGPENADDHLYIQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDS
EEEEHHHHHCCCCCCCCEEEEEEEHHHCCEEEECCCCCCEEEECHHHCCCCCCCEECCCC
HTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVNLIGKLKPFVSAKDIILEVLR
CCCCCCCEEEEEEECCCCEEEEEEECCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHH
RLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
HHCCCCCCCHHHHCCCCCEEEEECCCCHHHHHCCHHHCCCEECCCCHHHHHHHHHHHHCC
EDYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNS
CCCEEEEECCCCCCCCEEEEEHHHCCHHHCCCCCCCCEEEHHHHCCCEEEEEEEECCCCH
SYRDMLRTASILKGKTVSENVSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPC
HHHHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
IGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVAAVSALTGYITDPTELTDFED
CCCCCCCCCCHHEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHH
IEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
CCCCCEEEECCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCC
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAAL
CCCCCCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCH
APVYLGIKAVLAKSFARIHKANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
KIDIINKTKGIKYKTLLEATDRQKKMILKGGLLNLIKSSQQ
EEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]