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The map label for this gene is ptbA

Identifier: 170756331

GI number: 170756331

Start: 3033823

End: 3034302

Strand: Reverse

Name: ptbA

Synonym: CLD_1734

Alternate gene names: 170756331

Gene position: 3034302-3033823 (Counterclockwise)

Preceding gene: 170756295

Following gene: 170757859

Centisome position: 76.66

GC content: 31.46

Gene sequence:

>480_bases
ATGTTTGGCTTTTTTAATAGAGAACACAAGATTCTTGCTCCAGTTGCAGGGAGAGTATTAGAATTATCTGAAGTGCCAGA
TGAAGTATTTGCAAGTAAATTAGCAGGAGATGGTGTTGCTATTGATTGTGAAGATGATATTATTGTGGCTCCAGCAGATG
GAGTAATTTCTTTAATATTTAAAACTAATCATGCTTTTGGAATTATATTAAAAGATGGAACAGAGCTTTTAGTTCATATT
GGTATTGACACTGTTAAATTAGAAGGCAAAGGATTTGAAAGATTATTACAGGAAGGCGATAAGGTTAAAGCAGGAGATCC
TATTATTAAGATAGATAGGGAGTTTATTAAGTCTCAAGGATATTCTTTGATTACGCCTGTGTTAATAACAAATCCTGATA
ATGTTAAAAATACAGAATATAAAACAGGTTTTAATGCTAAGCCAGGAAAGGATATTTTGATTAGCTATACTGATAAATAA

Upstream 100 bases:

>100_bases
AATCAGGCATGAGTTAGGGATAACTTATGCCTTTTTTTTATTTGAATTTTTATAAAATGTAGGCAATATCTGTATTACCT
ATATTTAGGAGGTTTTAAAA

Downstream 100 bases:

>100_bases
TTCATAAACTTATTTATAATGTTAAATATAAAGTTTATTTATTAAGGCGTAATACTAATATAATTTATGGATATTGGTAT
TACGCCTTTTGGTATAAAAA

Product: PTS system, glucose subfamily, IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MFGFFNREHKILAPVAGRVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIFKTNHAFGIILKDGTELLVHI
GIDTVKLEGKGFERLLQEGDKVKAGDPIIKIDREFIKSQGYSLITPVLITNPDNVKNTEYKTGFNAKPGKDILISYTDK

Sequences:

>Translated_159_residues
MFGFFNREHKILAPVAGRVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIFKTNHAFGIILKDGTELLVHI
GIDTVKLEGKGFERLLQEGDKVKAGDPIIKIDREFIKSQGYSLITPVLITNPDNVKNTEYKTGFNAKPGKDILISYTDK
>Mature_159_residues
MFGFFNREHKILAPVAGRVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIFKTNHAFGIILKDGTELLVHI
GIDTVKLEGKGFERLLQEGDKVKAGDPIIKIDREFIKSQGYSLITPVLITNPDNVKNTEYKTGFNAKPGKDILISYTDK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=125, Percent_Identity=49.6, Blast_Score=136, Evalue=6e-34,
Organism=Escherichia coli, GI1788757, Length=128, Percent_Identity=47.65625, Blast_Score=127, Evalue=3e-31,
Organism=Escherichia coli, GI1790159, Length=124, Percent_Identity=45.1612903225806, Blast_Score=105, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17386; Mature: 17386

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGFFNREHKILAPVAGRVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIF
CCCCCCCCCEEEECCCCCEEEHHCCCHHHHHHHHCCCCEEEECCCCEEEECCCCEEEEEE
KTNHAFGIILKDGTELLVHIGIDTVKLEGKGFERLLQEGDKVKAGDPIIKIDREFIKSQG
ECCCEEEEEEECCCEEEEEECCEEEEECCCCHHHHHHCCCCCCCCCCEEEECHHHHHCCC
YSLITPVLITNPDNVKNTEYKTGFNAKPGKDILISYTDK
CEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MFGFFNREHKILAPVAGRVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIF
CCCCCCCCCEEEECCCCCEEEHHCCCHHHHHHHHCCCCEEEECCCCEEEECCCCEEEEEE
KTNHAFGIILKDGTELLVHIGIDTVKLEGKGFERLLQEGDKVKAGDPIIKIDREFIKSQG
ECCCEEEEEEECCCEEEEEECCEEEEECCCCHHHHHHCCCCCCCCCCEEEECHHHHHCCC
YSLITPVLITNPDNVKNTEYKTGFNAKPGKDILISYTDK
CEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]