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The map label for this gene is thiD

Identifier: 170756299

GI number: 170756299

Start: 569080

End: 569880

Strand: Reverse

Name: thiD

Synonym: CLD_0291

Alternate gene names: 170756299

Gene position: 569880-569080 (Counterclockwise)

Preceding gene: 170755892

Following gene: 170755281

Centisome position: 14.4

GC content: 32.71

Gene sequence:

>801_bases
ATGAAAAAAGTTTTAACTATAGCCGGCTCAGATAGCTCGGGAGGTGCTGGCATTCAAGCAGATATTAAAACTATGAGCTC
TTTAGGAGTATATGCTATGAGCATAATAACTGCTGTAACCGCTCAAAATTCCATAGGTGTACAAGATGTTCATGAAGTGC
CAAAGAACATGGTTGAAGCCCAAATCAAATCTGTATTTGATGATATAGATGTAGATGCTGTAAAAATAGGTATGCTGTCC
AATTCTGAAACTATAAAATCTATAAAAGAATACTTAGAAAAATATAAAGCTAAAAATATAGTTTTAGATCCTGTTATGGT
TTCTAAAAGTGGTTATTTTCTTCTTAAGCCAGAGGCTATAGAAGAACTAAAAAAATTAATTTCTATAACTAACATTGTAA
CCCCTAATATACCAGAGGCTGAAGTTCTAAGCGCCATGAAAATCAATTCAGAAGATGATATGAAGAAAGCTGCTACTATA
ATCCAAGCTATGGGAGTTAAAAACGTTTTAGTTAAAGGTGGCCATAGATGCAGTGATGCAAATGACATACTTCTTTATGA
AGATAAATTTATAACAATCCCTGGTAACAGAATAGAAACAAAAAATACTCATGGTACTGGCTGTACATTATCCTCTGCTA
TAGCTTCTTATTTAGCTAAAGGCTTTAACATAGAAAAATCAGTATCTTTATCTAAAGAATATATTACAAAAGCTATCGAG
AATTCCTTCCCTATTGGTGAAGGCGTAGGTCCTGTTGGACATTTTATAGAGCTTTATAAAAAAGCACATTTAGATTTCTA
A

Upstream 100 bases:

>100_bases
TTAAACTTACTTAAAATAAATCTATTATTATTAAATAACATTGACTTTACTATTGCAGAAACAAACTTAAATTAATTAAT
TACCTAGGAGGTTTTTTATC

Downstream 100 bases:

>100_bases
GAATTTCTTCTGTGATACTTATTTAAGTTTAATTAATAAGAATCTATAAATTAAACTTAAAAACATGGACAATCATTATT
ATGGTAAAGCTACATGAAAT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKKVLTIAGSDSSGGAGIQADIKTMSSLGVYAMSIITAVTAQNSIGVQDVHEVPKNMVEAQIKSVFDDIDVDAVKIGMLS
NSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAIEELKKLISITNIVTPNIPEAEVLSAMKINSEDDMKKAATI
IQAMGVKNVLVKGGHRCSDANDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
NSFPIGEGVGPVGHFIELYKKAHLDF

Sequences:

>Translated_266_residues
MKKVLTIAGSDSSGGAGIQADIKTMSSLGVYAMSIITAVTAQNSIGVQDVHEVPKNMVEAQIKSVFDDIDVDAVKIGMLS
NSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAIEELKKLISITNIVTPNIPEAEVLSAMKINSEDDMKKAATI
IQAMGVKNVLVKGGHRCSDANDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
NSFPIGEGVGPVGHFIELYKKAHLDF
>Mature_266_residues
MKKVLTIAGSDSSGGAGIQADIKTMSSLGVYAMSIITAVTAQNSIGVQDVHEVPKNMVEAQIKSVFDDIDVDAVKIGMLS
NSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAIEELKKLISITNIVTPNIPEAEVLSAMKINSEDDMKKAATI
IQAMGVKNVLVKGGHRCSDANDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
NSFPIGEGVGPVGHFIELYKKAHLDF

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=269, Percent_Identity=43.1226765799257, Blast_Score=223, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6325378, Length=273, Percent_Identity=35.5311355311355, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324517, Length=276, Percent_Identity=36.9565217391304, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324997, Length=281, Percent_Identity=35.5871886120996, Blast_Score=123, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28760; Mature: 28760

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLTIAGSDSSGGAGIQADIKTMSSLGVYAMSIITAVTAQNSIGVQDVHEVPKNMVEA
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QIKSVFDDIDVDAVKIGMLSNSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAI
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEECCEEECCCCEEEECHHHH
EELKKLISITNIVTPNIPEAEVLSAMKINSEDDMKKAATIIQAMGVKNVLVKGGHRCSDA
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCC
NDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
CCEEEEECEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NSFPIGEGVGPVGHFIELYKKAHLDF
CCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKVLTIAGSDSSGGAGIQADIKTMSSLGVYAMSIITAVTAQNSIGVQDVHEVPKNMVEA
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QIKSVFDDIDVDAVKIGMLSNSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAI
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEECCEEECCCCEEEECHHHH
EELKKLISITNIVTPNIPEAEVLSAMKINSEDDMKKAATIIQAMGVKNVLVKGGHRCSDA
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCC
NDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
CCEEEEECEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NSFPIGEGVGPVGHFIELYKKAHLDF
CCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]