BacMap

Definition	Clostridium botulinum B1 str. Okra, complete genome.
Accession	NC_010516
Length	3,958,233

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The map label for this gene is crt [H]

Identifier: 170756239

GI number: 170756239

Start: 3479292

End: 3480074

Strand: Reverse

Name: crt [H]

Synonym: CLD_1329

Alternate gene names: 170756239

Gene position: 3480074-3479292 (Counterclockwise)

Preceding gene: 170755649

Following gene: 170755442

Centisome position: 87.92

GC content: 31.8

Gene sequence:

>783_bases
GTGGAAAGTAAAAATATCTTATTAGATAAAAAAAATCATATTGCTATTTTAAGTTTAAATAGAGAATCCGTACTTAATGC
TTTAAACACTCGGGTATTAAAAGAGATTAATACAATTTTAGATGATATAGAAAATGATGAAGATATTTATGTAGTTATAG
TAACTGGAAAAGGAAAGGCCTTTATAGCTGGAGCTGATATCTCAGAAATGAAAGATAAAACTCCTAAGGAAGGAAGAATA
TTTGGAGAATTAGGACAGAACATATGTAGAAAAATAGAAACCATGGAAAAGCCAACTATAGCAGCCGTAAATGGATATGC
CTTAGGCGGTGGATGCGAATTAGCAATGAGTTGTGATATAAGAATAGCTTCAGAAAAAGCTAAATTCGGTCAACCAGAAG
TAGGTTTAGGAATAACTCCAGGATTCGGAGGAACACAAAGGCTTTCCCGCTTAGTAGGGTTAAGTAAGGCAAAGGAATTG
ATACTTACCACAGATATTATAAAATCTCAGGAAGCCTTAAGCATAGGGCTTGTTAATAAAGTAGTATCAGCAGAAAGTTT
ATTAGAAGAAGCAATAGCACTAGCCGAAAAAATAGTATCTAAACCTCAATCTGCTGTAAGATATGCTAAATCAGCTATAA
ATAGGGGTTATGAAACAGATATTGAAACAGGAATGATAATTGAAAAGGATTTATTTGGTTTATGTTTTGCAACAGAAGAC
CAAAAAGAAGGAATGGAAGCTTTTTTAGAAAAAAGAAAACCAAATTTTATTAATAAATATTAA

Upstream 100 bases:

>100_bases
GCTTATTATTGTTAAAATAATAGCTATTAATTTTTGCGCAAAAACAGATTACATATACTGGCATGAAAGTTGCTTCTTAT
AAAAATAAGGGGGTGGTTAA

Downstream 100 bases:

>100_bases
ATTTTAAGGGGGCATAAAAATGAAAGTATTTATTTTAGGTGGCGGAACAATGGGAGCAGGCATCGTTGAAGTTTTTGCTA
AATCTGGCCATGAAGTTGTT

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MESKNILLDKKNHIAILSLNRESVLNALNTRVLKEINTILDDIENDEDIYVVIVTGKGKAFIAGADISEMKDKTPKEGRI
FGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDIRIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKEL
ILTTDIIKSQEALSIGLVNKVVSAESLLEEAIALAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
QKEGMEAFLEKRKPNFINKY

Sequences:

>Translated_260_residues
MESKNILLDKKNHIAILSLNRESVLNALNTRVLKEINTILDDIENDEDIYVVIVTGKGKAFIAGADISEMKDKTPKEGRI
FGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDIRIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKEL
ILTTDIIKSQEALSIGLVNKVVSAESLLEEAIALAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
QKEGMEAFLEKRKPNFINKY
>Mature_260_residues
MESKNILLDKKNHIAILSLNRESVLNALNTRVLKEINTILDDIENDEDIYVVIVTGKGKAFIAGADISEMKDKTPKEGRI
FGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDIRIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKEL
ILTTDIIKSQEALSIGLVNKVVSAESLLEEAIALAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
QKEGMEAFLEKRKPNFINKY

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=47.3684210526316, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=36.8627450980392, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI31542718, Length=239, Percent_Identity=34.7280334728033, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI68989263, Length=204, Percent_Identity=36.7647058823529, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=33.2, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI20127408, Length=215, Percent_Identity=35.8139534883721, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=29.1304347826087, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=29.1304347826087, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI70995211, Length=223, Percent_Identity=35.4260089686099, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI37594469, Length=254, Percent_Identity=31.1023622047244, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI37594471, Length=254, Percent_Identity=31.1023622047244, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI157694520, Length=200, Percent_Identity=29, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI261878539, Length=108, Percent_Identity=42.5925925925926, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI221136756, Length=258, Percent_Identity=24.4186046511628, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136753, Length=258, Percent_Identity=24.4186046511628, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI62530384, Length=257, Percent_Identity=24.5136186770428, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221307494, Length=202, Percent_Identity=28.2178217821782, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI116235487, Length=190, Percent_Identity=28.4210526315789, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4757966, Length=202, Percent_Identity=25.2475247524752, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538581, Length=202, Percent_Identity=25.2475247524752, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI25453481, Length=202, Percent_Identity=25.2475247524752, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538573, Length=202, Percent_Identity=25.2475247524752, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=25.2336448598131, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=25.2336448598131, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=25.2336448598131, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=202, Percent_Identity=24.7524752475248, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI49355787, Length=202, Percent_Identity=24.7524752475248, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI295842266, Length=255, Percent_Identity=22.3529411764706, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=40.6374501992032, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=39.5121951219512, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=34.8, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=31.9230769230769, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=36.3157894736842, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI87082183, Length=253, Percent_Identity=32.8063241106719, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=33.2, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=43.0830039525692, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=43.1451612903226, Blast_Score=197, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=39.0350877192982, Blast_Score=163, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17534483, Length=253, Percent_Identity=30.4347826086957, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=37.0165745856354, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17536985, Length=267, Percent_Identity=29.2134831460674, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=247, Percent_Identity=28.3400809716599, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=36.6666666666667, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=36.6666666666667, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=36.6666666666667, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=254, Percent_Identity=30.3149606299213, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=191, Percent_Identity=31.9371727748691, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=191, Percent_Identity=31.9371727748691, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=193, Percent_Identity=31.6062176165803, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=233, Percent_Identity=25.3218884120172, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=24.3801652892562, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=219, Percent_Identity=26.9406392694064, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=48.9878542510121, Blast_Score=227, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=48.9878542510121, Blast_Score=227, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=39.4422310756972, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21357171, Length=267, Percent_Identity=30.3370786516854, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24650670, Length=216, Percent_Identity=31.4814814814815, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=25.9842519685039, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19920382, Length=221, Percent_Identity=32.1266968325792, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24654903, Length=193, Percent_Identity=27.4611398963731, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19921000, Length=176, Percent_Identity=33.5227272727273, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583077, Length=176, Percent_Identity=33.5227272727273, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583079, Length=176, Percent_Identity=33.5227272727273, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921018, Length=189, Percent_Identity=29.6296296296296, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583165, Length=186, Percent_Identity=30.6451612903226, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28571730, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571729, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550169, Length=198, Percent_Identity=25.7575757575758, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28413; Mature: 28413

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKNILLDKKNHIAILSLNRESVLNALNTRVLKEINTILDDIENDEDIYVVIVTGKGKA
CCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
FIAGADISEMKDKTPKEGRIFGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDI
EEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCEEEEEECE
RIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKELILTTDIIKSQEALSIGLVNK
EEECCHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VVSAESLLEEAIALAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHEEEEECCC
QKEGMEAFLEKRKPNFINKY
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MESKNILLDKKNHIAILSLNRESVLNALNTRVLKEINTILDDIENDEDIYVVIVTGKGKA
CCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
FIAGADISEMKDKTPKEGRIFGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDI
EEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCEEEEEECE
RIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKELILTTDIIKSQEALSIGLVNK
EEECCHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VVSAESLLEEAIALAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHEEEEECCC
QKEGMEAFLEKRKPNFINKY
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]