Click here to switch to the map view.

The map label for this gene is citS [H]

Identifier: 170756137

GI number: 170756137

Start: 310481

End: 312103

Strand: Direct

Name: citS [H]

Synonym: CLD_0516

Alternate gene names: 170756137

Gene position: 310481-312103 (Clockwise)

Preceding gene: 170757488

Following gene: 170755347

Centisome position: 7.84

GC content: 29.27

Gene sequence:

>1623_bases
ATGAGAAAAGCAATGAAGCTACAAACGAAAATTACCTTATTAATTATTACAGTGGTTTTTCTTTCTATAGCTATAATTAT
TTCATTTGTTGTTTCATGGATGACTGGAAACATTGAAAATAAAGCTAAAACTAATATTATGAATGTAGCGGAGATGGTAG
CGCATTCTACACACATTGTAGATGCTTTAGAAAAAAAGGATCCTAACGGAAAGATTGGACCTTATATAAACATGCAGCTT
AAAAATTTACAACAGATTCAGTATATAATTGTAGCAGATAATGATGGAATTAGATATTCTCATCCAAATCCTAAAATGAT
AAAGAAGAAATTTGAAGGAGGGGATGAGTATAAGGTAGTAAAAGAAGGAGAAACGTATATATCTGAAGCTACAGGTACAT
TAGGAAAATCCATGAGGGCTTTTGCTCCAATATATGATAGAGAAAACAAAAGAGAGATAGGTTTTGTTTCCGTAGGCACA
CTTACTGAAAATATTGAAACGGCTAAGCATACAGCTATTTTATATATAATTTTAATTGGGTTTGGAGGGCTTATGGCAGG
AACAATAGGGGCAATATTACTTTCTAATAATATAAAAAATATTTTATTAGGACTAGAGCCAGAAGAAATAACAAATCTTT
ATAATGAGAAGATGGGAATTTTGGATACTATCCATGAAGGATTAGTAGCAGTTGATTATATGGGAAGAATTACCCTTATA
AATAATTCAGCTTTAAAAATATTAAATCTTCAACATAAAGTTGATAAGGATGAAGTCATAGGAAAAAATATAGAAAATAT
TATTGAAAATACAGGAATGATTAATATATTACAAACGGGAGAATCTGAATTTGAAAGAGAGCAGAAAATAAATAATACAA
CTATAATGACCAATAGAATTCCTATAATGAAAAGAGAAAAGATCGTGGGAGCTATTGCTAGTTTTAGGGATAAAACAGAA
GTGACTAGAATGGCAGAAGAACTTACAGGTGCCAAAAAAATGGCATGGTCTCTAAGAGCTCAAAATCATGAATTCATGAA
CAAACTTCATACTATTTCAGGACTTATACAGCTAGAAGAATATGATGAAGCTCTACAGTTTATTTCAGATATGGCTAAGA
GTAGAAATAATATAAGTAATATATTATCCCATAATATAAAAAATCCTTCTATATCTGCTTTATTGTTGTCAAAATATAAT
AAAGCTGAAGAGTGTAGAGTTAGTTTAAAAATAGATGAGAATTGTAAACTTACAAAATTACCACAGCATATGACCTCAGA
AGAAATTGTATCAATTATAGGTAATTTAATAGAGAATTCTTTAGATGAAGTTAAAAATGATGGTACTGGATTAATATATA
TAAAAATAGTAGAAAATAAAAATTATTTAAATATAATAGTTAAGGACAATGGTAGTGGAATTCCAGTAGAATATAGAGAA
AAAATATATAATCAAGGTTTTTCAACAAAGGAAGCACAGCGTGGATATGGAATGTATATAGTAAAAAAAATAATTGATGA
GACTAAAGGAACAATAAGACTTGATGTGGACAACGGTGTTATTTGGAATATAACTATACCGATGACAAGGGGTGATAAAA
TTGATTCGGGTAATGATAGTTGA

Upstream 100 bases:

>100_bases
ATTAATCTTTATATGTGTATCTATTGTCCTTTATGATTTCATGTTTTTTAAATTTGTAATTGTAAAATCTAAATTTATAT
ATTAAGGCTAAGGTGAGAAT

Downstream 100 bases:

>100_bases
AGATGATCCTATGGTTAGAGAAATAAATTCTAAATTTTTAAAAAGAGTAGAAGGATTTGTTTTGTATAAAGCAGTTTCCA
ATCTTTATGACGCTAAAAAA

Product: putative sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTHIVDALEKKDPNGKIGPYINMQL
KNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVVKEGETYISEATGTLGKSMRAFAPIYDRENKREIGFVSVGT
LTENIETAKHTAILYIILIGFGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRIPIMKREKIVGAIASFRDKTE
VTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEEYDEALQFISDMAKSRNNISNILSHNIKNPSISALLLSKYN
KAEECRVSLKIDENCKLTKLPQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
KIYNQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS

Sequences:

>Translated_540_residues
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTHIVDALEKKDPNGKIGPYINMQL
KNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVVKEGETYISEATGTLGKSMRAFAPIYDRENKREIGFVSVGT
LTENIETAKHTAILYIILIGFGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRIPIMKREKIVGAIASFRDKTE
VTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEEYDEALQFISDMAKSRNNISNILSHNIKNPSISALLLSKYN
KAEECRVSLKIDENCKLTKLPQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
KIYNQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS
>Mature_540_residues
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTHIVDALEKKDPNGKIGPYINMQL
KNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVVKEGETYISEATGTLGKSMRAFAPIYDRENKREIGFVSVGT
LTENIETAKHTAILYIILIGFGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRIPIMKREKIVGAIASFRDKTE
VTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEEYDEALQFISDMAKSRNNISNILSHNIKNPSISALLLSKYN
KAEECRVSLKIDENCKLTKLPQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
KIYNQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=540, Percent_Identity=31.6666666666667, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1786837, Length=536, Percent_Identity=26.865671641791, Blast_Score=228, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60672; Mature: 60672

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTHIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DALEKKDPNGKIGPYINMQLKNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVV
HHHHCCCCCCCCCCEEEHHHHCCCEEEEEEEECCCCEEECCCCHHHHHHHCCCCCCEEEE
KEGETYISEATGTLGKSMRAFAPIYDRENKREIGFVSVGTLTENIETAKHTAILYIILIG
ECCCHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHH
FGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
HHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEE
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRI
ECCCEEEEEHHHHCCHHHHHHCHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCEEEECCC
PIMKREKIVGAIASFRDKTEVTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YDEALQFISDMAKSRNNISNILSHNIKNPSISALLLSKYNKAEECRVSLKIDENCKLTKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCEECC
PQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
CHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHH
KIYNQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS
HHHHCCCCCCHHHCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTHIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DALEKKDPNGKIGPYINMQLKNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVV
HHHHCCCCCCCCCCEEEHHHHCCCEEEEEEEECCCCEEECCCCHHHHHHHCCCCCCEEEE
KEGETYISEATGTLGKSMRAFAPIYDRENKREIGFVSVGTLTENIETAKHTAILYIILIG
ECCCHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHH
FGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
HHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEE
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRI
ECCCEEEEEHHHHCCHHHHHHCHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCEEEECCC
PIMKREKIVGAIASFRDKTEVTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YDEALQFISDMAKSRNNISNILSHNIKNPSISALLLSKYNKAEECRVSLKIDENCKLTKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCEECC
PQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
CHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHH
KIYNQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS
HHHHCCCCCCHHHCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]