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The map label for this gene is glcD [H]

Identifier: 170756040

GI number: 170756040

Start: 1121023

End: 1122420

Strand: Direct

Name: glcD [H]

Synonym: CLD_3557

Alternate gene names: 170756040

Gene position: 1121023-1122420 (Clockwise)

Preceding gene: 170754986

Following gene: 170757098

Centisome position: 28.32

GC content: 32.69

Gene sequence:

>1398_bases
ATGGAATATAAAAAATTAGATGTTAAAGATATAGAGTTTTTAAAAAGTGTAGCAGGACAGGAAAGAGTATATACAGGTGA
AGATATAAATGAAGACTATAGTCATGATGAACTTGGTGGAATAAGTAAAATGCCTGACGCCATGGTAGAAGTTTTAAGTA
CAGAAGAAGTATCAAAAATAATGGCCTATGCCCATAAGAATAACATTCCAGTGGTTGTAAGAGGATCAGGAACTGGACTT
GTAGGGGCATCCGTTCCAATTCATGGTGGAATAATGATAAACATGACAAAGATGAATAGGATATTAGAGATAGATGAAGA
AAATTTAACTCTTACTGTAGAGCCAGGGGTTCTTCTTATGGAGATTGGAAAATTTGTAGAAGAACATGATCTTTTTTATC
CACCAGATCCAGGTGAAAAATCAGCAACTATAGGTGGAAATATAAGTACTAATGCTGGTGGAATGAGAGCAGTAAAATAT
GGGGTTACAAGAGATTATATAAGAGGATTAGAAATAGTTCTTCCAGATGGTAAAGTTATTCAAGTTGGTGGAAAGGTAGT
TAAGAATAGTTCAGGATACAGCATAAAGGATTTAGTTTGTGGCGCAGAAGGAACTTTAGCTATAGTTACAAAAGCTATAT
TAAAACTATTACCATTGCCAAAGAAGGCTATAAGTTTGCTTATACCATTCCCAAACTTAGAAATGGCAATAAATACTGTG
CCAAAAATAATAAAATCAAAAGCAATACCAACTGCTATAGAATTCATGCAAAGGGAAGTTATATTAGCAGCAGAAAAATT
TTTAGGAAAGAAGTTCCCAGACAATTCTTCAGATGCGTATTTATTGCTAACTTTTGATGGAAATACTAAAGAAGACATAG
AAAAAGATTATGAAAAAGTGGCAGATATTTGTTTAGAAGAAGGAGCTCTTGACGTATATATTTCAGATACGGATGAAAGA
AAAGAAGCAGTATGGTCAGCCAGAGGTGCATTCCTTGAAGCAGTTAAAGCATCTACTACAGAAATGGATGAATGTGATGT
TGTAGTTCCAAGAAATAAAGTGGCAAGTTTTGTAAAATATACAGATGAACTTCAAGAACAATTTGATGTAAGGATAAGAA
GTTTTGGTCATGCAGGAGATGGAAATTTACATGTATATGTATTAAGAGATAATTTAAATAAAGAACAATGGGACAAAAAA
TTAAAGGATGTATTTGAATGTATGTACAAAAAATCTGTAGAATTAAATGGGCTAGTTTCTGGGGAGCATGGTATAGGCTT
TGCTAAAAAACCTTATTTATTTGAGCAATATGGTGAAGAATACATGGAGCTTATGAAAAATATTAAGTTAGCATTTGATC
CTAAAAATATATTAAACCCAGGTAAAGTTTGTCAGTAA

Upstream 100 bases:

>100_bases
TATAAATAATATAAAAGAAGGTAAAAGATTAACTGTATAATTGCTAGAGTATTTAAGGACCAATTAACTAACTGATAATT
CAATTTTGGAGGTAATTATT

Downstream 100 bases:

>100_bases
AATAATAAAGCTTTTTAGAGTGAAAAATGGAGAAGCACATGAATATTTTTTTAATTCATGTGTTTCTTCATTTTTTATTT
GCATGAAACATTAGTATTAG

Product: putative glycolate oxidase

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MEYKKLDVKDIEFLKSVAGQERVYTGEDINEDYSHDELGGISKMPDAMVEVLSTEEVSKIMAYAHKNNIPVVVRGSGTGL
VGASVPIHGGIMINMTKMNRILEIDEENLTLTVEPGVLLMEIGKFVEEHDLFYPPDPGEKSATIGGNISTNAGGMRAVKY
GVTRDYIRGLEIVLPDGKVIQVGGKVVKNSSGYSIKDLVCGAEGTLAIVTKAILKLLPLPKKAISLLIPFPNLEMAINTV
PKIIKSKAIPTAIEFMQREVILAAEKFLGKKFPDNSSDAYLLLTFDGNTKEDIEKDYEKVADICLEEGALDVYISDTDER
KEAVWSARGAFLEAVKASTTEMDECDVVVPRNKVASFVKYTDELQEQFDVRIRSFGHAGDGNLHVYVLRDNLNKEQWDKK
LKDVFECMYKKSVELNGLVSGEHGIGFAKKPYLFEQYGEEYMELMKNIKLAFDPKNILNPGKVCQ

Sequences:

>Translated_465_residues
MEYKKLDVKDIEFLKSVAGQERVYTGEDINEDYSHDELGGISKMPDAMVEVLSTEEVSKIMAYAHKNNIPVVVRGSGTGL
VGASVPIHGGIMINMTKMNRILEIDEENLTLTVEPGVLLMEIGKFVEEHDLFYPPDPGEKSATIGGNISTNAGGMRAVKY
GVTRDYIRGLEIVLPDGKVIQVGGKVVKNSSGYSIKDLVCGAEGTLAIVTKAILKLLPLPKKAISLLIPFPNLEMAINTV
PKIIKSKAIPTAIEFMQREVILAAEKFLGKKFPDNSSDAYLLLTFDGNTKEDIEKDYEKVADICLEEGALDVYISDTDER
KEAVWSARGAFLEAVKASTTEMDECDVVVPRNKVASFVKYTDELQEQFDVRIRSFGHAGDGNLHVYVLRDNLNKEQWDKK
LKDVFECMYKKSVELNGLVSGEHGIGFAKKPYLFEQYGEEYMELMKNIKLAFDPKNILNPGKVCQ
>Mature_465_residues
MEYKKLDVKDIEFLKSVAGQERVYTGEDINEDYSHDELGGISKMPDAMVEVLSTEEVSKIMAYAHKNNIPVVVRGSGTGL
VGASVPIHGGIMINMTKMNRILEIDEENLTLTVEPGVLLMEIGKFVEEHDLFYPPDPGEKSATIGGNISTNAGGMRAVKY
GVTRDYIRGLEIVLPDGKVIQVGGKVVKNSSGYSIKDLVCGAEGTLAIVTKAILKLLPLPKKAISLLIPFPNLEMAINTV
PKIIKSKAIPTAIEFMQREVILAAEKFLGKKFPDNSSDAYLLLTFDGNTKEDIEKDYEKVADICLEEGALDVYISDTDER
KEAVWSARGAFLEAVKASTTEMDECDVVVPRNKVASFVKYTDELQEQFDVRIRSFGHAGDGNLHVYVLRDNLNKEQWDKK
LKDVFECMYKKSVELNGLVSGEHGIGFAKKPYLFEQYGEEYMELMKNIKLAFDPKNILNPGKVCQ

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=459, Percent_Identity=31.5904139433551, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI37595754, Length=482, Percent_Identity=30.0829875518672, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI119964728, Length=431, Percent_Identity=31.554524361949, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI4501993, Length=506, Percent_Identity=26.4822134387352, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1789351, Length=449, Percent_Identity=33.630289532294, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1787977, Length=497, Percent_Identity=23.943661971831, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI48994907, Length=493, Percent_Identity=23.3265720081136, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994914, Length=167, Percent_Identity=30.5389221556886, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71992373, Length=461, Percent_Identity=31.0195227765727, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71992381, Length=461, Percent_Identity=29.7180043383948, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI71992392, Length=381, Percent_Identity=30.7086614173228, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17534361, Length=472, Percent_Identity=30.0847457627119, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17556096, Length=467, Percent_Identity=24.8394004282655, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6320023, Length=485, Percent_Identity=29.4845360824742, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6320027, Length=420, Percent_Identity=29.5238095238095, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6320764, Length=488, Percent_Identity=29.0983606557377, Blast_Score=190, Evalue=5e-49,
Organism=Drosophila melanogaster, GI18921117, Length=472, Percent_Identity=31.7796610169492, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24639277, Length=472, Percent_Identity=31.7796610169492, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24639275, Length=472, Percent_Identity=31.7796610169492, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24653753, Length=248, Percent_Identity=28.2258064516129, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 51609; Mature: 51609

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKKLDVKDIEFLKSVAGQERVYTGEDINEDYSHDELGGISKMPDAMVEVLSTEEVSKI
CCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHH
MAYAHKNNIPVVVRGSGTGLVGASVPIHGGIMINMTKMNRILEIDEENLTLTVEPGVLLM
HHHHHCCCCCEEEECCCCEEEECCCCCCCCEEEEEHHCCCEEEECCCCEEEEECCCCHHH
EIGKFVEEHDLFYPPDPGEKSATIGGNISTNAGGMRAVKYGVTRDYIRGLEIVLPDGKVI
HHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCHHHHCCEEEEECCCCEE
QVGGKVVKNSSGYSIKDLVCGAEGTLAIVTKAILKLLPLPKKAISLLIPFPNLEMAINTV
EECCEEEECCCCCCHHHEEECCCCHHHHHHHHHHHHCCCCHHHHEEEECCCCHHHHHHHH
PKIIKSKAIPTAIEFMQREVILAAEKFLGKKFPDNSSDAYLLLTFDGNTKEDIEKDYEKV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHH
ADICLEEGALDVYISDTDERKEAVWSARGAFLEAVKASTTEMDECDVVVPRNKVASFVKY
HHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHH
TDELQEQFDVRIRSFGHAGDGNLHVYVLRDNLNKEQWDKKLKDVFECMYKKSVELNGLVS
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
GEHGIGFAKKPYLFEQYGEEYMELMKNIKLAFDPKNILNPGKVCQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCHHHCCCCCCCC
>Mature Secondary Structure
MEYKKLDVKDIEFLKSVAGQERVYTGEDINEDYSHDELGGISKMPDAMVEVLSTEEVSKI
CCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHH
MAYAHKNNIPVVVRGSGTGLVGASVPIHGGIMINMTKMNRILEIDEENLTLTVEPGVLLM
HHHHHCCCCCEEEECCCCEEEECCCCCCCCEEEEEHHCCCEEEECCCCEEEEECCCCHHH
EIGKFVEEHDLFYPPDPGEKSATIGGNISTNAGGMRAVKYGVTRDYIRGLEIVLPDGKVI
HHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCHHHHCCEEEEECCCCEE
QVGGKVVKNSSGYSIKDLVCGAEGTLAIVTKAILKLLPLPKKAISLLIPFPNLEMAINTV
EECCEEEECCCCCCHHHEEECCCCHHHHHHHHHHHHCCCCHHHHEEEECCCCHHHHHHHH
PKIIKSKAIPTAIEFMQREVILAAEKFLGKKFPDNSSDAYLLLTFDGNTKEDIEKDYEKV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHH
ADICLEEGALDVYISDTDERKEAVWSARGAFLEAVKASTTEMDECDVVVPRNKVASFVKY
HHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHH
TDELQEQFDVRIRSFGHAGDGNLHVYVLRDNLNKEQWDKKLKDVFECMYKKSVELNGLVS
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
GEHGIGFAKKPYLFEQYGEEYMELMKNIKLAFDPKNILNPGKVCQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]