| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is cphA
Identifier: 170755898
GI number: 170755898
Start: 155502
End: 158123
Strand: Direct
Name: cphA
Synonym: CLD_0666
Alternate gene names: 170755898
Gene position: 155502-158123 (Clockwise)
Preceding gene: 170755043
Following gene: 170754962
Centisome position: 3.93
GC content: 29.75
Gene sequence:
>2622_bases ATGAAAATAGAAAATATAAGAGTATTTGAGGGACGAAATATTTATTCTCATAAAAAATGTATAAGAATGGATGTGGACTT GGAAGGGTATAGTAATATATCTAGCAAAGAAATACAAGAATTTAACAAGACTCTTTTGAATTATGTTCCAGAATTAAGAG AACATTGCTGTTGTGTAGGTAAAAAAGGTGGCTTTGTAGAAAGACTTTATGAAGGAACTTACTTATCTCATATATGTGAA CACGTAATAATAGCATTACAAAATAGAATTGGGATAGATGTAAGTTATGGTAAAGCAAGAGAAATAGAAGGAGAAAAATA TTATATAATTTATCAATATAAATATAAAAATATGGCTATTGAGTGTGGAAAAATAGCTGTAGATTTAATAAACAATATAA TCAATGGTAAAAGATATAATATAAAAACTAAAACAAGAGAATTGGTATGTTTACTGAAGACTGAAGAACTTGGTCCCAGT ACTTTATCCATAATACAAGAAGCTAAAAAAAGAAATATTCCCGTTACCAAAATAGGTGAAGATAGCATGTTTCAATTAGG ATATGGTATCAAAGGAGAAACTATAGAGGCAACTATATGTAATAGCACTAGTGCTGTATCTGTAGATATAGCTTGCGATA AATTACTATCTAAAAATATATTGATGGATCAGTGTATACCTGTTGCCGAAGGATATAAAGTAAAAAATTATATTGATTTA CTGTTTAAAGCAGAAAAATTAGGCTATCCAGTAGTTTTAAAACCACGTTTTGGTAACCAAGGCAAGGGTGTAGTAGTAAA TATAAAAAACCAAAAGGAATTAGTAAATGCTTACAGTATAGTAAACAAAAAATTCCAAGACATAATGATAGAGAAATATA TCAATGGCAAAGATTACAGGGCATGTGTTGTAGATGGAAAGGTGGTAGCTGTAGCCCAACGAATTCCACCATACATAATA GGCAATGGTAAAAGTACAATTTATGAATTGATAAAGGAATTAAATAGGGATGAAAGAAGAGGAGATGGACATGAAAAACC ATTAACTAAAGTAAAAATAGATAAAGATCTTAAAAATAATATAAATAAAGAAGGATATACTTTAGGCTATATACTTCCTG AGGGATATAAATTAGAATTAAGGCATAATGCCAATTTATCTACTGGAGGAGTAGCTATAGATTGTACTGACTTAATATGC ACTGAAACAAGAGAGGTTTGCGAGAGAGTTGCTAAAGCTATAGGGTTAAATATATGTGGTATAGATATATGTTGTAGTGA TATAAGTAAGCCACTTAAAGAGAACGAAGGGATAATGGAAGTAAATGCGGCACCTGGCATAAGAATGCATCAATATCCTT ATAAAGGGAAAAGCAGAAATGTAGCAAAGGCCATAGTAGATATGATGTTTAAGGAATATGATGGCAATATACCTATTATA TCAATTACAGGAACTAATGGAAAAACTACAACTACTAGATTGATAGCTCATATTTTAAGTTTTTCAGGTAAGAAGGTGGG AATGACAACCACCGGTGGTATATATATAAACAATAAATGTATAAACAAGGGAGATACTACAGGATACTATAGTGCTAAGA CTGTATTAACCAATAAAGAGGTAGAAGTAGCTGTTTTAGAATTGGCAAGGGGAGGATTAATTAGAGATGGTTTGCCTTAT GATTTGGCGGATGTAGGTATAATAACTAATGTTACAGAGGATCATTTGGGTCTAGGAGGTATAAATACCTTAGAGGATAT GGCTTACGTTAAAGCATTAGTAGGTGAAGCTGTTAAAAAAGATGGATATGTAGTAATAAATGCTGATGATGAAGCTAGCA TAAGCATTATAAATAGGATAAAAAGCAAAATTATATTATTTACTAAGAATAAAAATAACCCCATCATATCCCAACATTTA GACAATAAAAATTTAGTGTTATATTTAGATGAAGATACTATATATTTAAAGAAATTAAATAAAAATGAGGAAATAATAAA TGTTAACAAAATACCGATAACCTTAGGGGGAAAACTAATTTATAATGTAGAAAATGCAATGGCAGCTATAGCAGCATTAA TAGCATTAGGGCTAGATGTAAATACTATTAGACAAGGCCTAGAAAGTTTTAGTAATGAAGAACAAAATCCTGGTAGATTT AATATGTATAATGTACATGGAACAAATGTAATATTAGATTATGGACACAACATTGAAGGATATAAAGTGGTTTTAGAAAG TATTAAAAAAATTAACCATAAAAGAATAATAGGAGTTGTAGGAGTGCCAGGAGATAGAACTAATAGTAGCACATTAAAAG TAGGTAATATTTGTGGAGAAAATTTTGATTATGTCTATATAAAGGAAGATAGGGACAAAAGAGGAAGAAAACATGGAGAA ATAGCAGATTTATTGAAAAAAGGAATTTTAGAAACGGGATTTAAAAATTCTAAGTTAAATATAATATTAGATGAGGAAGA AGCTCTTAAAAAGGCTATAGAATTCTCTAACCCAGGAGATTTAGTTATAATGTTTTTTGAAGAATTTGAACCGTCAGAAA ATATTGTAAAAGATAAAATTAAAAAAGGCAAGATAACAAAACGTGAAACAGCTTTAGCATAG
Upstream 100 bases:
>100_bases TTACATGTACTTAAAAATAGAGATAAGTTTGATTTGAACTATAGAAGACCTATATAACAAAGCATATATGTTTTTGGAAA AGAGGAGGAAATGTTACAAC
Downstream 100 bases:
>100_bases GATTAAAGCTACAATTTATGAATATCATGATTGTAGCTTTAATTTTTTTGATGTATTATATATTCTATAATATATATTTT TGGAGGAATAAAATGTTATC
Product: cyanophycin synthetase
Products: NA
Alternate protein names: Cyanophycin synthase [H]
Number of amino acids: Translated: 873; Mature: 873
Protein sequence:
>873_residues MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIQEFNKTLLNYVPELREHCCCVGKKGGFVERLYEGTYLSHICE HVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAIECGKIAVDLINNIINGKRYNIKTKTRELVCLLKTEELGPS TLSIIQEAKKRNIPVTKIGEDSMFQLGYGIKGETIEATICNSTSAVSVDIACDKLLSKNILMDQCIPVAEGYKVKNYIDL LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQKELVNAYSIVNKKFQDIMIEKYINGKDYRACVVDGKVVAVAQRIPPYII GNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNNINKEGYTLGYILPEGYKLELRHNANLSTGGVAIDCTDLIC TETREVCERVAKAIGLNICGIDICCSDISKPLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII SITGTNGKTTTTRLIAHILSFSGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKEVEVAVLELARGGLIRDGLPY DLADVGIITNVTEDHLGLGGINTLEDMAYVKALVGEAVKKDGYVVINADDEASISIINRIKSKIILFTKNKNNPIISQHL DNKNLVLYLDEDTIYLKKLNKNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFSNEEQNPGRF NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGENFDYVYIKEDRDKRGRKHGE IADLLKKGILETGFKNSKLNIILDEEEALKKAIEFSNPGDLVIMFFEEFEPSENIVKDKIKKGKITKRETALA
Sequences:
>Translated_873_residues MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIQEFNKTLLNYVPELREHCCCVGKKGGFVERLYEGTYLSHICE HVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAIECGKIAVDLINNIINGKRYNIKTKTRELVCLLKTEELGPS TLSIIQEAKKRNIPVTKIGEDSMFQLGYGIKGETIEATICNSTSAVSVDIACDKLLSKNILMDQCIPVAEGYKVKNYIDL LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQKELVNAYSIVNKKFQDIMIEKYINGKDYRACVVDGKVVAVAQRIPPYII GNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNNINKEGYTLGYILPEGYKLELRHNANLSTGGVAIDCTDLIC TETREVCERVAKAIGLNICGIDICCSDISKPLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII SITGTNGKTTTTRLIAHILSFSGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKEVEVAVLELARGGLIRDGLPY DLADVGIITNVTEDHLGLGGINTLEDMAYVKALVGEAVKKDGYVVINADDEASISIINRIKSKIILFTKNKNNPIISQHL DNKNLVLYLDEDTIYLKKLNKNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFSNEEQNPGRF NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGENFDYVYIKEDRDKRGRKHGE IADLLKKGILETGFKNSKLNIILDEEEALKKAIEFSNPGDLVIMFFEEFEPSENIVKDKIKKGKITKRETALA >Mature_873_residues MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIQEFNKTLLNYVPELREHCCCVGKKGGFVERLYEGTYLSHICE HVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAIECGKIAVDLINNIINGKRYNIKTKTRELVCLLKTEELGPS TLSIIQEAKKRNIPVTKIGEDSMFQLGYGIKGETIEATICNSTSAVSVDIACDKLLSKNILMDQCIPVAEGYKVKNYIDL LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQKELVNAYSIVNKKFQDIMIEKYINGKDYRACVVDGKVVAVAQRIPPYII GNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNNINKEGYTLGYILPEGYKLELRHNANLSTGGVAIDCTDLIC TETREVCERVAKAIGLNICGIDICCSDISKPLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII SITGTNGKTTTTRLIAHILSFSGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKEVEVAVLELARGGLIRDGLPY DLADVGIITNVTEDHLGLGGINTLEDMAYVKALVGEAVKKDGYVVINADDEASISIINRIKSKIILFTKNKNNPIISQHL DNKNLVLYLDEDTIYLKKLNKNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFSNEEQNPGRF NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGENFDYVYIKEDRDKRGRKHGE IADLLKKGILETGFKNSKLNIILDEEEALKKAIEFSNPGDLVIMFFEEFEPSENIVKDKIKKGKITKRETALA
Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) [H]
COG id: COG1181
COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ATP-grasp domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011761 - InterPro: IPR013651 - InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR011810 - InterPro: IPR004101 - InterPro: IPR013221 [H]
Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK [H]
EC number: =6.3.2.29; =6.3.2.30 [H]
Molecular weight: Translated: 97545; Mature: 97545
Theoretical pI: Translated: 8.55; Mature: 8.55
Prosite motif: PS50975 ATP_GRASP ; PS01011 FOLYLPOLYGLU_SYNT_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.2 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 2.2 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIQEFNKTLLNYVPELREHCCCVG CCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEC KKGGFVERLYEGTYLSHICEHVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAI CCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCEEEEEEEEEECCEEE ECGKIAVDLINNIINGKRYNIKTKTRELVCLLKTEELGPSTLSIIQEAKKRNIPVTKIGE EHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECC DSMFQLGYGIKGETIEATICNSTSAVSVDIACDKLLSKNILMDQCIPVAEGYKVKNYIDL CCCEEECCCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCHHHHHCCCCCCCEEHHHHHHH LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQKELVNAYSIVNKKFQDIMIEKYINGKDYR HHHHHHCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE ACVVDGKVVAVAQRIPPYIIGNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNN EEEECCEEEEEHHHCCCEEEECCHHHHHHHHHHHCCHHHCCCCCCCCCEEEEECCHHHCC INKEGYTLGYILPEGYKLELRHNANLSTGGVAIDCTDLICTETREVCERVAKAIGLNICG CCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEE IDICCSDISKPLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII HHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEE SITGTNGKTTTTRLIAHILSFSGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKE EEECCCCCHHHHHHHHHHHHCCCCEECEEECCCEEECCEECCCCCCCCEEEEEEEEECCC VEVAVLELARGGLIRDGLPYDLADVGIITNVTEDHLGLGGINTLEDMAYVKALVGEAVKK CEEEEEEHHCCCCCCCCCCCCHHHCCEEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHHC DGYVVINADDEASISIINRIKSKIILFTKNKNNPIISQHLDNKNLVLYLDEDTIYLKKLN CCEEEEECCCCCHHHHHHHHCCEEEEEEECCCCCCHHEECCCCCEEEEECCCEEEEEECC KNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFSNEEQNPGRF CCCCEEEEEEEEEEECCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCE NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGE EEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCC NFDYVYIKEDRDKRGRKHGEIADLLKKGILETGFKNSKLNIILDEEEALKKAIEFSNPGD CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCC LVIMFFEEFEPSENIVKDKIKKGKITKRETALA EEEEEEECCCCCHHHHHHHHHCCCCCCHHHCCC >Mature Secondary Structure MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIQEFNKTLLNYVPELREHCCCVG CCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEC KKGGFVERLYEGTYLSHICEHVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAI CCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCEEEEEEEEEECCEEE ECGKIAVDLINNIINGKRYNIKTKTRELVCLLKTEELGPSTLSIIQEAKKRNIPVTKIGE EHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECC DSMFQLGYGIKGETIEATICNSTSAVSVDIACDKLLSKNILMDQCIPVAEGYKVKNYIDL CCCEEECCCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCHHHHHCCCCCCCEEHHHHHHH LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQKELVNAYSIVNKKFQDIMIEKYINGKDYR HHHHHHCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE ACVVDGKVVAVAQRIPPYIIGNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNN EEEECCEEEEEHHHCCCEEEECCHHHHHHHHHHHCCHHHCCCCCCCCCEEEEECCHHHCC INKEGYTLGYILPEGYKLELRHNANLSTGGVAIDCTDLICTETREVCERVAKAIGLNICG CCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEE IDICCSDISKPLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII HHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEE SITGTNGKTTTTRLIAHILSFSGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKE EEECCCCCHHHHHHHHHHHHCCCCEECEEECCCEEECCEECCCCCCCCEEEEEEEEECCC VEVAVLELARGGLIRDGLPYDLADVGIITNVTEDHLGLGGINTLEDMAYVKALVGEAVKK CEEEEEEHHCCCCCCCCCCCCHHHCCEEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHHC DGYVVINADDEASISIINRIKSKIILFTKNKNNPIISQHLDNKNLVLYLDEDTIYLKKLN CCEEEEECCCCCHHHHHHHHCCEEEEEEECCCCCCHHEECCCCCEEEEECCCEEEEEECC KNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFSNEEQNPGRF CCCCEEEEEEEEEEECCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCE NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGE EEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCC NFDYVYIKEDRDKRGRKHGEIADLLKKGILETGFKNSKLNIILDEEEALKKAIEFSNPGD CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCC LVIMFFEEFEPSENIVKDKIKKGKITKRETALA EEEEEEECCCCCHHHHHHHHHCCCCCCHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8905231 [H]