Click here to switch to the map view.

The map label for this gene is prfB

Identifier: 170755895

GI number: 170755895

Start: 210656

End: 211633

Strand: Direct

Name: prfB

Synonym: CLD_0606

Alternate gene names: 170755895

Gene position: 210656-211633 (Clockwise)

Preceding gene: 170755666

Following gene: 170756784

Centisome position: 5.32

GC content: 29.75

Gene sequence:

>978_bases
ATGCAAAATCCCAATTTTTGGAATGATATAAAAGAGGCACAAAAAATAACTTCAGAAGAAAAGTATTTAAAGGATAAGTT
AATCAAATATAATCATTTAAGAAATACTATAGAAGATATTATATCTCTTTGTGAAATGTTATCAGAAGAAGATGATGATA
TGAAAAAAGAAATAATATCAGAATATAAAAATATAAAAGAAGAAATAGATAAATTTAAAATAGAAACCCTATTATCTGGA
GAGTATGATAGAAATAATGTAATAATGACCCTACATTCAGGAGTAGGAGGAACAGATGCGCAGGATTGGACAGATATGCT
ATTTAGAATGTATACAAGGTGGGCAGAAAAAGAAGGATTTACTATTAAGATTATGGATTTATTAGAAGCTGAAGATGCAG
GTATAAAAAGTGTAACCCTAAAGATTACTGGAGAATTTGTATATGGATATCTTAAGGGTGAAAAGGGAATACACAGATTG
GTTAGAATATCTCCTTTTAATGCAAATGGTAAAAGACAGACTTCCTTTGCATCTATAGAAATATTACCAGAACTAACAGA
GGATCAGGAAATAGAAATAAAGAGTGAAGACTTAAAAATAGATACCTATAGAGCAGGTGGAGCTGGAGGACAGCATGTTA
ATAAAACTGAATCTGCAGTAAGAATAACTCATATACCAACAGGAATTATAGTACAATGTCAAAATGAAAGAAGTCAGCAT
ACTAATAAAGAAACAGCTATGAAGGTTTTGAAATCAAAATTAGTAGAACTAAAGGAAAGATCTCATAAAGAAAAAATAGA
AGATCTAACAGGAGAACTAAAGGATATGGGATGGGGAAGTCAAATAAGATCTTATGTATTTCATCCTTATAATCTAGTAA
AGGACCATAGAACAGGAGCAGAAACTAGCAATGTAGATTCAGTGATGAATGGTGATATAAATATATTCATAACACAGTAT
CTAAAAGGAAATTTTTAA

Upstream 100 bases:

>100_bases
ATAAATAATTCCTATGAACTTAAAAATACAATAAAAGAAATCGGGGAGTCTCTTTGACATAGAAAAATTAAAAAATAGTA
TATCAGAATTACAAAATAAA

Downstream 100 bases:

>100_bases
AATTAAAAGCTAAAAAATAAAGGGGATGCCTTAAAATAGATTTAATTTCAACATCATAAATATAAAAATTAAATCTATTT
TATTTTGAGACATCCCCTTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MQNPNFWNDIKEAQKITSEEKYLKDKLIKYNHLRNTIEDIISLCEMLSEEDDDMKKEIISEYKNIKEEIDKFKIETLLSG
EYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGFTIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRL
VRISPFNANGKRQTSFASIEILPELTEDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGAETSNVDSVMNGDINIFITQY
LKGNF

Sequences:

>Translated_325_residues
MQNPNFWNDIKEAQKITSEEKYLKDKLIKYNHLRNTIEDIISLCEMLSEEDDDMKKEIISEYKNIKEEIDKFKIETLLSG
EYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGFTIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRL
VRISPFNANGKRQTSFASIEILPELTEDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGAETSNVDSVMNGDINIFITQY
LKGNF
>Mature_325_residues
MQNPNFWNDIKEAQKITSEEKYLKDKLIKYNHLRNTIEDIISLCEMLSEEDDDMKKEIISEYKNIKEEIDKFKIETLLSG
EYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGFTIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRL
VRISPFNANGKRQTSFASIEILPELTEDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGAETSNVDSVMNGDINIFITQY
LKGNF

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=36.4620938628159, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI34577120, Length=288, Percent_Identity=33.3333333333333, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI166795305, Length=186, Percent_Identity=36.5591397849462, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI2367172, Length=329, Percent_Identity=46.8085106382979, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1787462, Length=240, Percent_Identity=40, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=314, Percent_Identity=28.9808917197452, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=30.3514376996805, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19921226, Length=282, Percent_Identity=35.1063829787234, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37382; Mature: 37382

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPNFWNDIKEAQKITSEEKYLKDKLIKYNHLRNTIEDIISLCEMLSEEDDDMKKEIIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EYKNIKEEIDKFKIETLLSGEYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGF
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
TIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRLVRISPFNANGKRQTSFASIE
EEEEEEHHCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHEEECCCCCCCCCCCCEEEEE
ILPELTEDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
EECCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHC
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCC
ETSNVDSVMNGDINIFITQYLKGNF
CCCCCHHHHCCCEEEEEEECCCCCC
>Mature Secondary Structure
MQNPNFWNDIKEAQKITSEEKYLKDKLIKYNHLRNTIEDIISLCEMLSEEDDDMKKEIIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EYKNIKEEIDKFKIETLLSGEYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGF
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
TIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRLVRISPFNANGKRQTSFASIE
EEEEEEHHCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHEEECCCCCCCCCCCCEEEEE
ILPELTEDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
EECCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHC
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCC
ETSNVDSVMNGDINIFITQYLKGNF
CCCCCHHHHCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA