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The map label for this gene is purB

Identifier: 170755650

GI number: 170755650

Start: 2512367

End: 2513797

Strand: Reverse

Name: purB

Synonym: CLD_2245

Alternate gene names: 170755650

Gene position: 2513797-2512367 (Counterclockwise)

Preceding gene: 170755446

Following gene: 170757318

Centisome position: 63.51

GC content: 31.24

Gene sequence:

>1431_bases
ATGAGAGATATTTATAATAATCCCTTAAACAAAAGATATTCATCTAAAAAAATGAGCTATTTATTTTCAGAAGAAATGAA
ATTTAAAACTTGGAGAAAATTATGGGTAGCTCTAGCAGAAAGCGAAAAGGAATTAGGTCTTAACATTACAGAAGAGCAAA
TAAATGAATTAAAAAAGTATATAGATGATATAAATTATGAAGTAGCGGAAGAAAGAGAAAAGGAAGTTCGACATGATGTT
ATGAGTCATGTATATGCTTATGGCGTTCAATGCCCTAAAGCAAAGGGTATAATACATTTAGGAGCTACTAGTTGTTATGT
AGGTGATAACACAGATTTAATTATAATGAAAGAAGCTATGATTTTAATAAAGAAGAAAATTATAAATGTTATAAATAATT
TGAAAAAGTTTGCCTTAGAATATAAGCATGTACCAACTTTAGGATTTACTCATTTTCAACCGGCTCAACTTACTACAGTA
GGTAAAAGAGCAACCCTTTGGATGCAAGATTTAGTATTAGATTTAGAACAATTAGAATTTGTTATAGATACTTTAAGATT
TAGAGGGGTTAAAGGAACTACAGGTACCCAAGCCAGCTTTATGGAATTGTTTGATGGAGATGAAAGTAAAGTAAAAGCTT
TAGATAAAAAGGTAGCAGAAAAGATGGGCTTCCCAAAGGAATTTATGGTAACGGGCCAAACATATCCAAGAAAAGTTGAT
TCAACTATATTAAATACATTATCAGAAATAGCTCAAAGTGCATACAAATTTAGCAATGACTTAAGATTACTTCAAAGCAT
GAAGGAGATGGAAGAACCCTTCGAAAAAAATCAAATAGGTTCATCAGCTATGGCCTATAAGAGAAATCCAATGAGATCAG
AAAGAATGGGAGCCTTAGCAAGATACGTTGTAGTAGATGCTTTAAATCCAGCTATAACCTCTTCTACGCAATGGTTTGAA
AGAACACTAGATGATTCTGCTAATAAGAGAATATCTATAGCAGAAGCTTTCCTAGCTTTAGATGGAGTGCTTAAACTATA
TATGAATATATCAGAGAATATGGTAGTATACGAAAAAGTTATAGAAAGTCATGTGAAAACAGAACTTCCATTTATGGCTA
CGGAAAATATAATGATGGAAGCTGTTAAAAAGGGTGGAGATAGACAAGAGCTTCACGAAGTTATAAGAACTCATTCTATG
GAAGCTGCAAGAAGAGTTAAGCAGGAAGGATTATCTAATGATCTAATAGAAAGAATAATAGAAGATAAATCCTTTGAATT
AACCAAAGAAGAAATATTAGCTTTAATAGATCCTAAAAAATTTACAGGAAGAGCAGAAGGTCAAGTAGTAGATTTTATAG
AAGAAGTAGTAAATCCAATATTAGAACAGAACAAAGCAATGCTAGGAGAAAAAGCAGAAATAAATGTATAA

Upstream 100 bases:

>100_bases
AATGTACGAATATAAAGTTGAAAAAATACTATAATATATGTTATTATATGAATAAATAGAATAAATATATACATATTGTA
CGCATAGAGGAGATGGAGTA

Downstream 100 bases:

>100_bases
TATTTAGTATAAAAATTTTAAAAGGAGCCATAATCTTGGCTCCTTTTAAAATTTTTATATTAATCTTTGAGCTAAAGCAT
TTTCTAAGTTTAATTTTGAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MRDIYNNPLNKRYSSKKMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKYIDDINYEVAEEREKEVRHDV
MSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAMILIKKKIINVINNLKKFALEYKHVPTLGFTHFQPAQLTTV
GKRATLWMQDLVLDLEQLEFVIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALARYVVVDALNPAITSSTQWFE
RTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKVIESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSM
EAARRVKQEGLSNDLIERIIEDKSFELTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV

Sequences:

>Translated_476_residues
MRDIYNNPLNKRYSSKKMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKYIDDINYEVAEEREKEVRHDV
MSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAMILIKKKIINVINNLKKFALEYKHVPTLGFTHFQPAQLTTV
GKRATLWMQDLVLDLEQLEFVIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALARYVVVDALNPAITSSTQWFE
RTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKVIESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSM
EAARRVKQEGLSNDLIERIIEDKSFELTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV
>Mature_476_residues
MRDIYNNPLNKRYSSKKMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKYIDDINYEVAEEREKEVRHDV
MSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAMILIKKKIINVINNLKKFALEYKHVPTLGFTHFQPAQLTTV
GKRATLWMQDLVLDLEQLEFVIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALARYVVVDALNPAITSSTQWFE
RTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKVIESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSM
EAARRVKQEGLSNDLIERIIEDKSFELTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=474, Percent_Identity=58.0168776371308, Blast_Score=582, Evalue=1e-166,
Organism=Homo sapiens, GI183227688, Length=387, Percent_Identity=60.7235142118863, Blast_Score=513, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17508577, Length=465, Percent_Identity=41.9354838709677, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32564234, Length=345, Percent_Identity=46.9565217391304, Blast_Score=321, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6323391, Length=478, Percent_Identity=54.18410041841, Blast_Score=518, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24647570, Length=457, Percent_Identity=55.7986870897155, Blast_Score=529, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 54608; Mature: 54608

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00163 FUMARATE_LYASES ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIYNNPLNKRYSSKKMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
IDDINYEVAEEREKEVRHDVMSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAM
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEEHHHHH
ILIKKKIINVINNLKKFALEYKHVPTLGFTHFQPAQLTTVGKRATLWMQDLVLDLEQLEF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
VIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
HHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCHHHH
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCHHHHHHHHH
RYVVVDALNPAITSSTQWFERTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKV
HHHHHHHCCCHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSMEAARRVKQEGLSNDLIERII
HHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EDKSFELTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV
CCCCCCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRDIYNNPLNKRYSSKKMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
IDDINYEVAEEREKEVRHDVMSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAM
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEEHHHHH
ILIKKKIINVINNLKKFALEYKHVPTLGFTHFQPAQLTTVGKRATLWMQDLVLDLEQLEF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
VIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
HHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCHHHH
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCHHHHHHHHH
RYVVVDALNPAITSSTQWFERTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKV
HHHHHHHCCCHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSMEAARRVKQEGLSNDLIERII
HHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EDKSFELTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV
CCCCCCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]