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The map label for this gene is purD

Identifier: 170755490

GI number: 170755490

Start: 3109081

End: 3110322

Strand: Reverse

Name: purD

Synonym: CLD_1668

Alternate gene names: 170755490

Gene position: 3110322-3109081 (Counterclockwise)

Preceding gene: 170754463

Following gene: 170755752

Centisome position: 78.58

GC content: 30.92

Gene sequence:

>1242_bases
ATGAAAATATTGATAATAGGTTCTGGTGGAAGAGAGCATGCTATAGCTTGGAAGATAGGTAAAAATACTTTAGTAGAAAA
AATATTTTGTGCTCCAGGGAATGGATGCACTGCACTAGAGCATAAGTGTGAAAATATAAATGTTTCATCCGATGAAGAAC
TATTAGAATTTGCTTTGAAAAATAATGTGGATTTAACTATTGTAGGTCCAGAAGTACCTTTAATTAATGGCATAGTAGAT
TTATTTAAAGATAAGGGACTTAATATATTTGGACCAGATAAAAAAGCCGCTTTATTAGAGGGAAGTAAGGCTTTTGCAAA
GGACTTTATGAAAAAATATAATATTAAAACTGCAGATTATGAAGTTTTTGAAGAAGAGAGTAAGGCTTTAGAATACTTAA
AAAATAGTGAGCATCCAGTGGTAATAAAAGCAGATGGATTAGCAGCAGGTAAGGGTGTTGTTATAAGCAATTCTTTTGAG
GAAAGTAAAAGTACTTTAGAAGAATTTATGGCTAAAGATAAATTTAAAGGTGCAGGTAAAAAAGTTGTTATAGAAGAATT
TTTAGAAGGTCCAGAGGCTTCTATACTATCTATAACTGATGGGGAAACTATAAGACCTTTTATATCCTCCAAGGATCATA
AACAAATATATGATGGTGGATTAGGACCGAATACAGGAGGAATGGGAGTTATAGCTCCAAACCCTTACTGTACAGAAGAA
GTATTAAAGGATTTTAATGAAAATATATTAAAGCCAACCTTAAAAGGGCTACAGAAAGAAAATCTAAAATTTTCAGGAAT
AATTTTCTTTGGAATAATGATAACTAAAAAAGGACCATATCTTCTTGAATATAACTTAAGAATGGGAGATCCAGAAACAG
AAGCTGTTCTTCCTTTGATGGAAAGTGATTTACTAGATTTAATATTAATGTCAATAAATAAAGAATTAAAAAGTACAAAC
ATAAAGTGGAAAAAAGCTTATTCCACTTGTGTTATAGCAGCATCTAAAGGATATCCTGAAAGTTATGAAAAGGGGTTTCT
AATAAATGGGGTAGAAAATTCGGAGGGAATATTTGGAGCAGGAGTTAAATTAAAAGATAATAGACTGTTAACTAATGGAG
GAAGAGTACTGTGCACTCAAGCGATAGGAGAAACTTTAGAACAGTCTATAAATACGGCTTATGAAAAGATGGAAAAAATA
AATTTTGAAGGTATATATTATAGAAAAGACATAGGAAAATAA

Upstream 100 bases:

>100_bases
AGTACTGAGATGAAAAGAGATATTTTTTATAAAGAAATTTCATCTCAATATTAGAATTGATTAATGTATGGGAATTAAAT
ATATTAAAGGGGTAAAAAAC

Downstream 100 bases:

>100_bases
TATTAATAATAAATAGCAGATACATTATGATATAAACAATTATGTGTATCTGCTATTAGTAATTTTTAAATAATAAAAAT
GGGATTAACACAAATTTTTC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKILIIGSGGREHAIAWKIGKNTLVEKIFCAPGNGCTALEHKCENINVSSDEELLEFALKNNVDLTIVGPEVPLINGIVD
LFKDKGLNIFGPDKKAALLEGSKAFAKDFMKKYNIKTADYEVFEEESKALEYLKNSEHPVVIKADGLAAGKGVVISNSFE
ESKSTLEEFMAKDKFKGAGKKVVIEEFLEGPEASILSITDGETIRPFISSKDHKQIYDGGLGPNTGGMGVIAPNPYCTEE
VLKDFNENILKPTLKGLQKENLKFSGIIFFGIMITKKGPYLLEYNLRMGDPETEAVLPLMESDLLDLILMSINKELKSTN
IKWKKAYSTCVIAASKGYPESYEKGFLINGVENSEGIFGAGVKLKDNRLLTNGGRVLCTQAIGETLEQSINTAYEKMEKI
NFEGIYYRKDIGK

Sequences:

>Translated_413_residues
MKILIIGSGGREHAIAWKIGKNTLVEKIFCAPGNGCTALEHKCENINVSSDEELLEFALKNNVDLTIVGPEVPLINGIVD
LFKDKGLNIFGPDKKAALLEGSKAFAKDFMKKYNIKTADYEVFEEESKALEYLKNSEHPVVIKADGLAAGKGVVISNSFE
ESKSTLEEFMAKDKFKGAGKKVVIEEFLEGPEASILSITDGETIRPFISSKDHKQIYDGGLGPNTGGMGVIAPNPYCTEE
VLKDFNENILKPTLKGLQKENLKFSGIIFFGIMITKKGPYLLEYNLRMGDPETEAVLPLMESDLLDLILMSINKELKSTN
IKWKKAYSTCVIAASKGYPESYEKGFLINGVENSEGIFGAGVKLKDNRLLTNGGRVLCTQAIGETLEQSINTAYEKMEKI
NFEGIYYRKDIGK
>Mature_413_residues
MKILIIGSGGREHAIAWKIGKNTLVEKIFCAPGNGCTALEHKCENINVSSDEELLEFALKNNVDLTIVGPEVPLINGIVD
LFKDKGLNIFGPDKKAALLEGSKAFAKDFMKKYNIKTADYEVFEEESKALEYLKNSEHPVVIKADGLAAGKGVVISNSFE
ESKSTLEEFMAKDKFKGAGKKVVIEEFLEGPEASILSITDGETIRPFISSKDHKQIYDGGLGPNTGGMGVIAPNPYCTEE
VLKDFNENILKPTLKGLQKENLKFSGIIFFGIMITKKGPYLLEYNLRMGDPETEAVLPLMESDLLDLILMSINKELKSTN
IKWKKAYSTCVIAASKGYPESYEKGFLINGVENSEGIFGAGVKLKDNRLLTNGGRVLCTQAIGETLEQSINTAYEKMEKI
NFEGIYYRKDIGK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=419, Percent_Identity=41.0501193317422, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI209869995, Length=419, Percent_Identity=41.0501193317422, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI209869993, Length=419, Percent_Identity=41.0501193317422, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI28416899, Length=419, Percent_Identity=41.0501193317422, Blast_Score=348, Evalue=4e-96,
Organism=Escherichia coli, GI1790438, Length=420, Percent_Identity=45.9523809523809, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=40.0468384074941, Blast_Score=293, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6321203, Length=435, Percent_Identity=41.8390804597701, Blast_Score=335, Evalue=1e-92,
Organism=Drosophila melanogaster, GI62471619, Length=426, Percent_Identity=38.0281690140845, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24582400, Length=426, Percent_Identity=38.0281690140845, Blast_Score=299, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45512; Mature: 45512

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIIGSGGREHAIAWKIGKNTLVEKIFCAPGNGCTALEHKCENINVSSDEELLEFALK
CEEEEEECCCCCCEEEEEECCHHHHHEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHH
NNVDLTIVGPEVPLINGIVDLFKDKGLNIFGPDKKAALLEGSKAFAKDFMKKYNIKTADY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCHHHHCCCHHHHHHHHHHCCCCCCCH
EVFEEESKALEYLKNSEHPVVIKADGLAAGKGVVISNSFEESKSTLEEFMAKDKFKGAGK
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCC
KVVIEEFLEGPEASILSITDGETIRPFISSKDHKQIYDGGLGPNTGGMGVIAPNPYCTEE
EEHHHHHHCCCCCEEEEECCCCEECHHHCCCCCHHHHCCCCCCCCCCEEEECCCCCCHHH
VLKDFNENILKPTLKGLQKENLKFSGIIFFGIMITKKGPYLLEYNLRMGDPETEAVLPLM
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCEEEEEEEECCCCCHHHHHHHH
ESDLLDLILMSINKELKSTNIKWKKAYSTCVIAASKGYPESYEKGFLINGVENSEGIFGA
HHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCHHCCCEEEECCCCCCCEEEC
GVKLKDNRLLTNGGRVLCTQAIGETLEQSINTAYEKMEKINFEGIYYRKDIGK
CEEEECCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
>Mature Secondary Structure
MKILIIGSGGREHAIAWKIGKNTLVEKIFCAPGNGCTALEHKCENINVSSDEELLEFALK
CEEEEEECCCCCCEEEEEECCHHHHHEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHH
NNVDLTIVGPEVPLINGIVDLFKDKGLNIFGPDKKAALLEGSKAFAKDFMKKYNIKTADY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCHHHHCCCHHHHHHHHHHCCCCCCCH
EVFEEESKALEYLKNSEHPVVIKADGLAAGKGVVISNSFEESKSTLEEFMAKDKFKGAGK
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCC
KVVIEEFLEGPEASILSITDGETIRPFISSKDHKQIYDGGLGPNTGGMGVIAPNPYCTEE
EEHHHHHHCCCCCEEEEECCCCEECHHHCCCCCHHHHCCCCCCCCCCEEEECCCCCCHHH
VLKDFNENILKPTLKGLQKENLKFSGIIFFGIMITKKGPYLLEYNLRMGDPETEAVLPLM
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCEEEEEEEECCCCCHHHHHHHH
ESDLLDLILMSINKELKSTNIKWKKAYSTCVIAASKGYPESYEKGFLINGVENSEGIFGA
HHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCHHCCCEEEECCCCCCCEEEC
GVKLKDNRLLTNGGRVLCTQAIGETLEQSINTAYEKMEKINFEGIYYRKDIGK
CEEEECCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]