BacMap

Definition	Clostridium botulinum B1 str. Okra, complete genome.
Accession	NC_010516
Length	3,958,233

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The map label for this gene is yvgN [H]

Identifier: 170755448

GI number: 170755448

Start: 1090638

End: 1091483

Strand: Direct

Name: yvgN [H]

Synonym: CLD_3583

Alternate gene names: 170755448

Gene position: 1090638-1091483 (Clockwise)

Preceding gene: 170757135

Following gene: 170756652

Centisome position: 27.55

GC content: 32.39

Gene sequence:

>846_bases
ATGCAAGATATAAAAAGTTATACAATTTTAAATAATGGAGTAAAAATGCCTTGGCTTGGATTTGGCACTTATAAGGTTGA
AAATGGAAATACCGTTATTCAATCAGTAAAGGAAGCCCTTAAAATTGGATATGGTCATATAGATACTGCTTCTTATTATG
GGAACGAAGAAGGCGTTGGCACTGCAATAAAAGAAAGCAGAATTCCTAGAGAAGATATATTTTTAGTAAGCAAAGTTTGG
AATTCAGAGCAGGGTTATGATAAAACTTTAAAATCCTTTGAAGATTCTATAAAAAGACTTGGCACAGATTATCTTGATTT
ATATTTAATTCATTGGCCTCAACCTTTAAGCAAAGAAACGTGGAAGGCCTTAGAAAAACTTTATAAAGAAGGACGTGTAA
AGGCCATTGGAGTAAGTAATTTTTTAGTTAATCACTTAAAGTGGTTATTAGAGGATGCTGAAATTATGCCAATGGTAAAT
CAGGTGGAATTCCATCCTCAGCTTATTCAAAAGGACTTAATAGAGTTTTGTAGCAAAAACAATATACAGTTAGAAGCATG
GTCTCCATTAATGAGAGGGAAAGTATTCCAGATTGAACTTCTACAGGAGCTTGCTCAAAAATACGGAAAAACAATATCTC
AAATCGTTTTAAGATGGGATTTACAAATGGGTGTGGTTACTATCCCTAAATCAATAAACCCATCAAGAATAAAAGAAAAT
GCAGATATATTCGACTTTGAAATAAGTAAAGAAGATATGGATAAAATTCAGCAACTAGATAAAGGTTTAAGGGTGGGCTC
AGACCCTAATAAAGTTTATCCATGTCAAAGTATATCTAATAAATAG

Upstream 100 bases:

>100_bases
TAGAATTGTAGTGTACAAACTAACTTATGCAAGCTATTAAGTTGTGTTAAGTAATTATGAAAAAATAGCTTTAAAAATCA
TACTTATAAGGAGTGATTTA

Downstream 100 bases:

>100_bases
TATTATTAAGATTAATGTTCATATTTTATATAGTGAACAGAAGGTATTTTATAAAAGGGTTTTAAATAATGACAAGTATT
CATATAATGGGGGAATTAAT

Product: aldo/keto reductase family oxidoreductase

Products: NA

Alternate protein names: GR; Methylglyoxal reductase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MQDIKSYTILNNGVKMPWLGFGTYKVENGNTVIQSVKEALKIGYGHIDTASYYGNEEGVGTAIKESRIPREDIFLVSKVW
NSEQGYDKTLKSFEDSIKRLGTDYLDLYLIHWPQPLSKETWKALEKLYKEGRVKAIGVSNFLVNHLKWLLEDAEIMPMVN
QVEFHPQLIQKDLIEFCSKNNIQLEAWSPLMRGKVFQIELLQELAQKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK

Sequences:

>Translated_281_residues
MQDIKSYTILNNGVKMPWLGFGTYKVENGNTVIQSVKEALKIGYGHIDTASYYGNEEGVGTAIKESRIPREDIFLVSKVW
NSEQGYDKTLKSFEDSIKRLGTDYLDLYLIHWPQPLSKETWKALEKLYKEGRVKAIGVSNFLVNHLKWLLEDAEIMPMVN
QVEFHPQLIQKDLIEFCSKNNIQLEAWSPLMRGKVFQIELLQELAQKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK
>Mature_281_residues
MQDIKSYTILNNGVKMPWLGFGTYKVENGNTVIQSVKEALKIGYGHIDTASYYGNEEGVGTAIKESRIPREDIFLVSKVW
NSEQGYDKTLKSFEDSIKRLGTDYLDLYLIHWPQPLSKETWKALEKLYKEGRVKAIGVSNFLVNHLKWLLEDAEIMPMVN
QVEFHPQLIQKDLIEFCSKNNIQLEAWSPLMRGKVFQIELLQELAQKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK

Specific function: Reduces glyoxal and methylglyoxal. Is not involved in the vitamin B6 biosynthesis [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=38.6440677966102, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=38.6440677966102, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI223468663, Length=299, Percent_Identity=36.4548494983278, Blast_Score=184, Evalue=8e-47,
Organism=Homo sapiens, GI93277124, Length=314, Percent_Identity=35.3503184713376, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI310109922, Length=274, Percent_Identity=37.5912408759124, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=36.1486486486487, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI45446745, Length=295, Percent_Identity=35.5932203389831, Blast_Score=175, Evalue=3e-44,
Organism=Homo sapiens, GI4503285, Length=295, Percent_Identity=35.5932203389831, Blast_Score=175, Evalue=3e-44,
Organism=Homo sapiens, GI24497583, Length=310, Percent_Identity=35.8064516129032, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI310109920, Length=295, Percent_Identity=35.2542372881356, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=37.2881355932203, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI5453543, Length=295, Percent_Identity=35.2542372881356, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4502049, Length=303, Percent_Identity=37.6237623762376, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI291291012, Length=276, Percent_Identity=35.8695652173913, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=35.2727272727273, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI300116271, Length=273, Percent_Identity=34.7985347985348, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=35.0649350649351, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI207028673, Length=123, Percent_Identity=41.4634146341463, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI310109926, Length=123, Percent_Identity=40.650406504065, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI87082198, Length=267, Percent_Identity=44.1947565543071, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=32.5842696629214, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788081, Length=270, Percent_Identity=32.2222222222222, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788070, Length=317, Percent_Identity=24.2902208201893, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48994888, Length=258, Percent_Identity=26.7441860465116, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081735, Length=199, Percent_Identity=28.1407035175879, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787674, Length=253, Percent_Identity=24.5059288537549, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71998625, Length=285, Percent_Identity=40.3508771929825, Blast_Score=213, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17564128, Length=296, Percent_Identity=35.8108108108108, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=36.0294117647059, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17552492, Length=277, Percent_Identity=35.3790613718412, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=36.7647058823529, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17537077, Length=292, Percent_Identity=35.2739726027397, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537075, Length=297, Percent_Identity=35.016835016835, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17550248, Length=300, Percent_Identity=34.6666666666667, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17566692, Length=290, Percent_Identity=35.1724137931034, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=34.5454545454545, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17537079, Length=303, Percent_Identity=29.7029702970297, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17561300, Length=270, Percent_Identity=32.5925925925926, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17550246, Length=84, Percent_Identity=48.8095238095238, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320576, Length=285, Percent_Identity=38.9473684210526, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324694, Length=294, Percent_Identity=38.0952380952381, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319625, Length=294, Percent_Identity=37.4149659863946, Blast_Score=172, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6320079, Length=283, Percent_Identity=37.8091872791519, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321896, Length=317, Percent_Identity=32.4921135646688, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322556, Length=269, Percent_Identity=34.2007434944238, Blast_Score=162, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24663317, Length=291, Percent_Identity=41.9243986254296, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24657054, Length=297, Percent_Identity=37.037037037037, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24644950, Length=302, Percent_Identity=35.0993377483444, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=36.0544217687075, Blast_Score=187, Evalue=9e-48,
Organism=Drosophila melanogaster, GI45553081, Length=289, Percent_Identity=36.6782006920415, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI221378297, Length=322, Percent_Identity=33.2298136645963, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI20129731, Length=307, Percent_Identity=35.5048859934853, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI21356425, Length=294, Percent_Identity=35.3741496598639, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI281366140, Length=257, Percent_Identity=35.7976653696498, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24662781, Length=275, Percent_Identity=39.2727272727273, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24662785, Length=273, Percent_Identity=38.8278388278388, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24646155, Length=135, Percent_Identity=40, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.283 [H]

Molecular weight: Translated: 32337; Mature: 32337

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIKSYTILNNGVKMPWLGFGTYKVENGNTVIQSVKEALKIGYGHIDTASYYGNEEGVG
CCCCCCEEEECCCCCCCEECCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
TAIKESRIPREDIFLVSKVWNSEQGYDKTLKSFEDSIKRLGTDYLDLYLIHWPQPLSKET
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCCHHH
WKALEKLYKEGRVKAIGVSNFLVNHLKWLLEDAEIMPMVNQVEFHPQLIQKDLIEFCSKN
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHCCC
NIQLEAWSPLMRGKVFQIELLQELAQKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
CEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCC
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK
CCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQDIKSYTILNNGVKMPWLGFGTYKVENGNTVIQSVKEALKIGYGHIDTASYYGNEEGVG
CCCCCCEEEECCCCCCCEECCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
TAIKESRIPREDIFLVSKVWNSEQGYDKTLKSFEDSIKRLGTDYLDLYLIHWPQPLSKET
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCCHHH
WKALEKLYKEGRVKAIGVSNFLVNHLKWLLEDAEIMPMVNQVEFHPQLIQKDLIEFCSKN
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHCCC
NIQLEAWSPLMRGKVFQIELLQELAQKYGKTISQIVLRWDLQMGVVTIPKSINPSRIKEN
CEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCC
ADIFDFEISKEDMDKIQQLDKGLRVGSDPNKVYPCQSISNK
CCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]