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The map label for this gene is greA

Identifier: 170755319

GI number: 170755319

Start: 801962

End: 802426

Strand: Direct

Name: greA

Synonym: CLD_0083

Alternate gene names: 170755319

Gene position: 801962-802426 (Clockwise)

Preceding gene: 170755297

Following gene: 170757057

Centisome position: 20.26

GC content: 30.32

Gene sequence:

>465_bases
ATGAAAAACATATTGACAGAAGAAAATATGAATAAATTAAAAGAAGAGTTAGAATATAGAATGACTAAAAAAAGAGCTGA
AATAGCAAAGGAAAAATTGGAAGCAGCTGCTCATGGTGATAGGTCAGAAAATGCAGAATATAAAGAAGCCTGTGCAAACT
ATAGAGAAAATGACAATAGAATTCAATATCTATTAACTATGATTTCAACTGCAAGTGTAATAGATGAGAAAAATCAGGAT
AAGTCAGTACTAGGAGTTAATAGTAAAGCTAAAATTAAATTTGTAGAAGATGAATTTGAAACGAATGTATCACTAGTAAC
TACCATGGATGCAGAGCCAGAAAATATGCTTATAAGTGTAGAATCAGATTTAGGAAAAGCATTGATGGGGAAAAAAGTTG
GAGATGTAGCTGAAGTTGATGCTCCAGGTGAAAAATATACAGTAGAAGTATTAGAAATTATATAA

Upstream 100 bases:

>100_bases
ATAACAGAGTTATGCCCTAGTATAGCATAATATTTCATTAGTATTTTACTATATATTGTATAATATTAACATAACATATC
TTGGGGAGGATTATTTGTAC

Downstream 100 bases:

>100_bases
TTATTTGTTAACTTATCAGTTTTATATTCTTTCTGATTTAGATAGTAGATAACAATAACAAATAATATCAAATAGAGGGA
TTGTTCTTAGATTTAAATAA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNRIQYLLTMISTASVIDEKNQD
KSVLGVNSKAKIKFVEDEFETNVSLVTTMDAEPENMLISVESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII

Sequences:

>Translated_154_residues
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNRIQYLLTMISTASVIDEKNQD
KSVLGVNSKAKIKFVEDEFETNVSLVTTMDAEPENMLISVESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII
>Mature_154_residues
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNRIQYLLTMISTASVIDEKNQD
KSVLGVNSKAKIKFVEDEFETNVSLVTTMDAEPENMLISVESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=31.3725490196078, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=30.2631578947368, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17367; Mature: 17367

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCH
IQYLLTMISTASVIDEKNQDKSVLGVNSKAKIKFVEDEFETNVSLVTTMDAEPENMLISV
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCHHCCCEEEEEEECCCCCCEEEEE
ESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII
CHHHHHHHHCCCCCCHHCCCCCCCHHHHHEEECC
>Mature Secondary Structure
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCH
IQYLLTMISTASVIDEKNQDKSVLGVNSKAKIKFVEDEFETNVSLVTTMDAEPENMLISV
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCHHCCCEEEEEEECCCCCCEEEEE
ESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII
CHHHHHHHHCCCCCCHHCCCCCCCHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA