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The map label for this gene is dusC [H]

Identifier: 170755297

GI number: 170755297

Start: 800867

End: 801805

Strand: Direct

Name: dusC [H]

Synonym: CLD_0084

Alternate gene names: 170755297

Gene position: 800867-801805 (Clockwise)

Preceding gene: 170754315

Following gene: 170755319

Centisome position: 20.23

GC content: 25.56

Gene sequence:

>939_bases
ATGAAATATTATTTAGCACCAATGGAAGGAATTACGGGATACATATATAGAAATTCCTATAAAAAGTTTTTTAATAATAT
TGATAAGTATTTTACACCTTTTATTGTTCCAAATAAAAGTACAAGCCTTAGAACTAAAGAATTAAGGGATATGTTGCCAG
AAAATAATAAAGGTATGAATATAGTGCCTCAAATACTTACTAACGATTCAGAAGGTTTTATTATCACCGCTAGAAAGTTA
CAGCAATTAGGTTATAATGAAATTAATTTAAATTTAGGTTGTCCTGCTGGAACCGTTGTCTCAAAAAATAGAGGCTCAGG
ATTTCTAGCTAAAAGAGAAGAACTTGATATATTTTTAGATGAAATATTTAAAATAGATGATATGAAAATTTCTATAAAGA
CCAGAATAGGAAAAGATAGTCCAGAGGAATTTTATGAGTTAATAAAAATTTATAATAAATATCCTATAGAAGAGTTAATT
ATTCATCCTAGAACGCAAAAAGATTTTTATGGAAATAAACCTAATTTAGATGTATTTAAAGATGCATTATCTTTAAGTAG
CAATCCTATATGTTATAATGGTGATATCTTCACTGTTAGTGGTTATAATAAATTAATAAAAACTTTTCCTAAAGTAAAAA
CAATAATGCTAGGAAGAGGCATACTAGCCAATCCAGGGCTAATAAATGAGATAAAAAATAATACTACTATAGACAAAGAA
GTATTAAAAGAATTTCATGATGAAATTTTAAATAAATATATAGAATTATATAATGAAGATAGAAATGCACTATTTAGAAT
GAAAGAACTCTGGGGATATATGATTTACATATTTTCAAATAATAAAAAATATGCTAAGAAAATAAAGAAGGCACAAAGGT
TAAATTGTTATAATGCAGCTGTCTTAAGTTTATTTAGAGAGCAGGAAATTATAAAATAG

Upstream 100 bases:

>100_bases
TTATTTTAATAGTTTAATATAATTAATCAATATTGAGTAGATAATAAAGAAGAAGATTGATATAATATATTCTTTAGAAT
ATTTATAAAGGAGTTATTAC

Downstream 100 bases:

>100_bases
GTAGGATTATTTAGTAACTAATATTAAAATTTAAGAGAGCTCAACTTAAGGTTTCTATAACAGAGTTATGCCCTAGTATA
GCATAATATTTCATTAGTAT

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKYYLAPMEGITGYIYRNSYKKFFNNIDKYFTPFIVPNKSTSLRTKELRDMLPENNKGMNIVPQILTNDSEGFIITARKL
QQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLDEIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELI
IHPRTQKDFYGNKPNLDVFKDALSLSSNPICYNGDIFTVSGYNKLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIYIFSNNKKYAKKIKKAQRLNCYNAAVLSLFREQEIIK

Sequences:

>Translated_312_residues
MKYYLAPMEGITGYIYRNSYKKFFNNIDKYFTPFIVPNKSTSLRTKELRDMLPENNKGMNIVPQILTNDSEGFIITARKL
QQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLDEIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELI
IHPRTQKDFYGNKPNLDVFKDALSLSSNPICYNGDIFTVSGYNKLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIYIFSNNKKYAKKIKKAQRLNCYNAAVLSLFREQEIIK
>Mature_312_residues
MKYYLAPMEGITGYIYRNSYKKFFNNIDKYFTPFIVPNKSTSLRTKELRDMLPENNKGMNIVPQILTNDSEGFIITARKL
QQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLDEIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELI
IHPRTQKDFYGNKPNLDVFKDALSLSSNPICYNGDIFTVSGYNKLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIYIFSNNKKYAKKIKKAQRLNCYNAAVLSLFREQEIIK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788462, Length=297, Percent_Identity=23.9057239057239, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36385; Mature: 36385

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYLAPMEGITGYIYRNSYKKFFNNIDKYFTPFIVPNKSTSLRTKELRDMLPENNKGMN
CCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCCCCCE
IVPQILTNDSEGFIITARKLQQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLD
EEHHHHCCCCCCEEEEHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCEECHHHHHHHHH
EIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELIIHPRTQKDFYGNKPNLDVFK
HHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHH
DALSLSSNPICYNGDIFTVSGYNKLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
HHHHCCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHH
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIYIFSNNKKYAKKIKKAQRLNCYNAA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCCHHHHH
VLSLFREQEIIK
HHHHHHHHHHCC
>Mature Secondary Structure
MKYYLAPMEGITGYIYRNSYKKFFNNIDKYFTPFIVPNKSTSLRTKELRDMLPENNKGMN
CCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCCCCCE
IVPQILTNDSEGFIITARKLQQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLD
EEHHHHCCCCCCEEEEHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCEECHHHHHHHHH
EIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELIIHPRTQKDFYGNKPNLDVFK
HHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHH
DALSLSSNPICYNGDIFTVSGYNKLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
HHHHCCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHH
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIYIFSNNKKYAKKIKKAQRLNCYNAA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCCHHHHH
VLSLFREQEIIK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]