Click here to switch to the map view.

The map label for this gene is murE

Identifier: 170755176

GI number: 170755176

Start: 1621869

End: 1623320

Strand: Direct

Name: murE

Synonym: CLD_3093

Alternate gene names: 170755176

Gene position: 1621869-1623320 (Clockwise)

Preceding gene: 170754363

Following gene: 170756422

Centisome position: 40.97

GC content: 27.96

Gene sequence:

>1452_bases
ATGAATTTAAATTTAATACTAAAGTCATTAGAATATTCTATTATAAAAGAAGATAAAAAGGAAATAGAAAAGATAGAATA
TGATTCAAGAAAAGTTAAAGAGGGAGATTTATTTGTTTGTATAGAGGGATATGCAACAGATGGACATAAATATGCAAAAA
AAGCTTATGACAATGGAGCTAAAGTAATTGTTTGTGAGAAGGATTTAGAAGACTTATCCTATTATAAAGATTGTACTATA
ATAAAGGTTCCAGATAGTAGAAAAGCATTAGCTATAATGTCATCTAATTATTATGGTAACCCATCTAAACATATTAAAAT
AATAGGTATTACAGGAACAAATGGCAAAACTACATCTACTTTTATGATGAAAGCTATACTAGAAAAGGCAGGCTATAAAG
TTGGGCTTTTGGGAACTATAGCAAATTATATAGGAAATAAAAAAATAGAATCTCATAGAACTACTCCAGAATCTCTAGAA
CTACAAAAATTATTTAAAGATATGGTGGATGAAAAAGTAGATTATTGTGTAATGGAAGTATCTTCTCATTCTTTATATTT
AGATAGAGTTTATGGAGTAGAATTTAAAGAGGCTATATTTACAAATTTAACACAGGATCATTTGGACTTTCATAAAACCT
TTGAAAATTATTTTAATTCCAAACTAATTTTATTTAAAAATGCTCAAAATTCAGTGATAAATATAGATGATAGTTATGGA
GAAAAAGTATTAAAGAAAGCTTTAGGAAATAAAATAACCTATGGTGTAGAGAAAAATTGTGATTTAAAAGCGGAAAATTT
ACATATGCATTCTAGAGGAGTAGAATTTGATACTATATTTAAAAATGAAAAAGAAACAATAGCTTTAAATATACCAGGTA
AATATAATATATATAATGCTTTAGGAAGCATTGGGGCTTGTCTTTTGGAGGGCATCCCTTTAAAAACTATAAAAGAAGCA
TTAGAAGATATGCCGTCTGTACCAGGAAGATGTGAAATTGTAACTAAAAATTATAATTTAGGTTATGATGTTATAGTAGA
TTATGCCCATACTCCAGATGGATTAGAAAATATATTAAATACTGCTCGAGAATTTACAAAGGGAAGATTAATAAGTGTAT
ACGGATGCGGGGGAGATAGAGATAGAACTAAAAGACCAATTATGGGAAAAGTGGGGTCAAATTTAAGTGATATAGCTATA
ATAACATCAGATAATCCAAGAACAGAGGATCCTAAACTTATAATAAAAGATGTATTAGAAGGTATAGAAAAAGATAACTA
TATAGTAGTTGAAGGAAGACGAGATGCCATAAGAAAAGCTATGGAAATGGCAAAAGAAAATGATGTTATAGTAGTGGCAG
GAAAAGGACATGAGGATTATCAAATATTAAAGGATAAAACTATTCACTTTGATGAAAGGGAAGTAATAGAAGAGCTAATA
AAAGAAATATAA

Upstream 100 bases:

>100_bases
TATATAAATAAAAAAAATAAAGTTTTTTATTTACAAAAATTAAATATGATTTTATTGTTATTATATTAAGTGACAATAAT
TAAGATAAGGAAGTGTTAAA

Downstream 100 bases:

>100_bases
TTACTCTAAAAATGGGAGTGTTAACATGGAAAATATTAAACTAGAAGAAATAATAAAAGCAGTTAATGGTGAGTTAGTGA
TAACTGGAGAAAAAGATGAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNLNLILKSLEYSIIKEDKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGAKVIVCEKDLEDLSYYKDCTI
IKVPDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTSTFMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLE
LQKLFKDMVDEKVDYCVMEVSSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNALGSIGACLLEGIPLKTIKEA
LEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILNTAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAI
ITSDNPRTEDPKLIIKDVLEGIEKDNYIVVEGRRDAIRKAMEMAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
KEI

Sequences:

>Translated_483_residues
MNLNLILKSLEYSIIKEDKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGAKVIVCEKDLEDLSYYKDCTI
IKVPDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTSTFMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLE
LQKLFKDMVDEKVDYCVMEVSSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNALGSIGACLLEGIPLKTIKEA
LEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILNTAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAI
ITSDNPRTEDPKLIIKDVLEGIEKDNYIVVEGRRDAIRKAMEMAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
KEI
>Mature_483_residues
MNLNLILKSLEYSIIKEDKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGAKVIVCEKDLEDLSYYKDCTI
IKVPDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTSTFMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLE
LQKLFKDMVDEKVDYCVMEVSSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNALGSIGACLLEGIPLKTIKEA
LEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILNTAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAI
ITSDNPRTEDPKLIIKDVLEGIEKDNYIVVEGRRDAIRKAMEMAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
KEI

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=480, Percent_Identity=34.7916666666667, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1786274, Length=316, Percent_Identity=25.3164556962025, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54645; Mature: 54645

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNLILKSLEYSIIKEDKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCC
KVIVCEKDLEDLSYYKDCTIIKVPDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTST
EEEEECCCHHHHHHHCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCHHH
FMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLELQKLFKDMVDEKVDYCVMEV
HHHHHHHHHCCCEEEHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEE
SSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
CCCEEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCHHHH
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNA
HHHHHHHHCCCEEECCCCCCCCCHHCEEECCCCCEEHHEECCCCCEEEEECCCCCHHHHH
LGSIGACLLEGIPLKTIKEALEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILN
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
TAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAIITSDNPRTEDPKLIIKDVLE
HHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
GIEKDNYIVVEGRRDAIRKAMEMAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
HCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHH
KEI
HHC
>Mature Secondary Structure
MNLNLILKSLEYSIIKEDKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCC
KVIVCEKDLEDLSYYKDCTIIKVPDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTST
EEEEECCCHHHHHHHCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCHHH
FMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLELQKLFKDMVDEKVDYCVMEV
HHHHHHHHHCCCEEEHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEE
SSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
CCCEEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCHHHH
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNA
HHHHHHHHCCCEEECCCCCCCCCHHCEEECCCCCEEHHEECCCCCEEEEECCCCCHHHHH
LGSIGACLLEGIPLKTIKEALEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILN
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
TAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAIITSDNPRTEDPKLIIKDVLE
HHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
GIEKDNYIVVEGRRDAIRKAMEMAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
HCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHH
KEI
HHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA