Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 170754945

GI number: 170754945

Start: 3951601

End: 3952320

Strand: Reverse

Name: gidB

Synonym: CLD_0833

Alternate gene names: 170754945

Gene position: 3952320-3951601 (Counterclockwise)

Preceding gene: 170756087

Following gene: 170754545

Centisome position: 99.85

GC content: 27.08

Gene sequence:

>720_bases
ATGGAATTTTTCAACATACTACAATCAGCATGCAATGATGTTAATTTAGATTTTAATGATAAAAAATATAATCAGCTTAT
AAGTTATAAAAATTTAATACAGGAATGGAATAAGAAAATAAATCTAACAGCTATTGTTGAAGATGATGAAATAATAAAAA
AACATTTTATAGATTGTATAAAAATATTTAAATCTTCACCTATAGGAGAAGCTAAAAGTTTAATAGATATAGGTACAGGT
GCAGGATTTCCAGGCATCCCCATAAAAATATTAAAAGAAGACATAGAAATAACTTTATTGGATTCCCTACAAAAGAGAAT
AAATTTTTTAAATATTGTAATAGGAGAGCTACAATTAAAAAATATACAATGTTTACATGGAAGAGCAGAAGATTATGCAC
AGGAAATTCAGCATAGACAAAAATATGATATAGCAGTTTCCCGAGCAGTAGCGAATTTAGCAGTTTTAAGTGAATTTTGT
ATTCCTTTTGTAGAAAAAGGTGGATATTTTATAGCCATGAAAGGTCCGTCTGTAGAAGAGGAAATAACAGCAGCTACAAA
ATCTATAGAAATTTTAGGTGGAAAAATAGAGGATATAATAAAAATAGATATTGAAGATACAGATCTAAAACATAATCTAG
TAATCATAAAAAAAGTAAAAGAAACGGGAAAAAGGTATCCTAGGAAGCCCGGTATTATTAAAAAAAATCCTCTAAAATAA

Upstream 100 bases:

>100_bases
CTAGAATTTCAGGTGTATCTCCAGCAGATATATCAGTATTACTTATATATATGGAAAGAAAAGATAGAGAAAACTAGAAA
ATCCTTTTGGAGGTTTTTAA

Downstream 100 bases:

>100_bases
ATGTAATAATTTGCAATTAAAAAAATAATAAAAAAAGAAGGAAAATTACATTTAAAGAAGAAATGTAAATATTAGGAAGA
GAATAAGAGGGATGGTAGTA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCIKIFKSSPIGEAKSLIDIGTG
AGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLKNIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFC
IPFVEKGGYFIAMKGPSVEEEITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK

Sequences:

>Translated_239_residues
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCIKIFKSSPIGEAKSLIDIGTG
AGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLKNIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFC
IPFVEKGGYFIAMKGPSVEEEITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK
>Mature_239_residues
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCIKIFKSSPIGEAKSLIDIGTG
AGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLKNIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFC
IPFVEKGGYFIAMKGPSVEEEITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=218, Percent_Identity=33.9449541284404, Blast_Score=138, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 27096; Mature: 27096

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCI
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHH
KIFKSSPIGEAKSLIDIGTGAGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLK
HHHHCCCCCHHHHHHEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEC
NIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFCIPFVEKGGYFIAMKGPSVEE
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHH
EITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCI
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHH
KIFKSSPIGEAKSLIDIGTGAGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLK
HHHHCCCCCHHHHHHEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEC
NIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFCIPFVEKGGYFIAMKGPSVEE
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHH
EITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA