BacMap

Definition	Clostridium botulinum B1 str. Okra, complete genome.
Accession	NC_010516
Length	3,958,233

Click here to switch to the map view.

The map label for this gene is yhcG [H]

Identifier: 170754863

GI number: 170754863

Start: 1339791

End: 1340663

Strand: Direct

Name: yhcG [H]

Synonym: CLD_3362

Alternate gene names: 170754863

Gene position: 1339791-1340663 (Clockwise)

Preceding gene: 170757737

Following gene: 170754903

Centisome position: 33.85

GC content: 26.23

Gene sequence:

>873_bases
ATGAGTAATATAGTAATAAAAAATGTTACTAAATCATACCAAAGAAAAGAAGCGTTATCAAATATTTCTATTAACATAGA
AGAAGGAAAAATATATGGTCTTCTTGGGAGAAATGGAGCAGGAAAAACAACTCTGCTGCATCTTATAACTAATAGAGCCA
TTTTAGATAGTGGAGAAATCTTAATAGATGGTGAAAATGTTTATGAGAATAACAAGGCCCTTTCTAAAGTTTATTTTATG
GAAGAAAAAAATCTTTTCCCTGAATCAATGAAAATAGTAGATGTTTTTGAATGGACAAAAGAGTTTTATGAAGACTTTGA
TATGGAATATGCTATTAAACTTAGTGATCTTTTTAAATTAAATATAAAGAAAAAAGTAAAAAATTTATCAACAGGATATA
AATCAATAAGTAAAATTATTGCTACTTTAGCTTCAGGAGCAGAAATTTTAATTTTTGATGAACCTATTTTAGGCTTAGAT
GCTAATCATAGAGAACTTTTTTATAAAGAGTTATTAAGGATTTATGGAGAAGGGCAAAAAACAATTGTAATATCCACCCA
TATAATAGAGGAAATATCACATCTTTTAGAAAAAGTAGTTATACTAAAGGAAGGTAAAATAATAGAAAGTGATTCTGTTG
AAAATATGCTTAATTTAGCCTATGCAGTTTCAGGTAGTAGTGATGATGTAGATAAATATATAACCGCTAAAAACACAATA
GATATAGAGACTTTAGGCTCATATAAAAAAGCTACAATTTTGCAAGAAAGAACTAAAGAAGATTTAGATAATATAAAAAA
ACTAGAATTAGAAATTAGCACAGTAGAACTTCAAAAACTATTTATATATTTAACAAATATGGAGGAAAAATAG

Upstream 100 bases:

>100_bases
AAATTTTTTGATAGCTACATTTTAAGTCTTTTAGATGAAGCTAAAAGACTAGGAATAACTATAGATGAAATAATGGATAT
GATAAAAGGGGGATATGGCA

Downstream 100 bases:

>100_bases
ATGGAAAAAAGTAATAAAATTTGTAAATATCAATTAACGACTTCTATAAAACCTATATTGATTTATTATAGTATATTAAT
AGGATTCTTATTATTAATAT

Product: ABC transporter, ATP-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEILIDGENVYENNKALSKVYFM
EEKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSDLFKLNIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLD
ANHRELFYKELLRIYGEGQKTIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTI
DIETLGSYKKATILQERTKEDLDNIKKLELEISTVELQKLFIYLTNMEEK

Sequences:

>Translated_290_residues
MSNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEILIDGENVYENNKALSKVYFM
EEKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSDLFKLNIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLD
ANHRELFYKELLRIYGEGQKTIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTI
DIETLGSYKKATILQERTKEDLDNIKKLELEISTVELQKLFIYLTNMEEK
>Mature_289_residues
SNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEILIDGENVYENNKALSKVYFME
EKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSDLFKLNIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLDA
NHRELFYKELLRIYGEGQKTIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTID
IETLGSYKKATILQERTKEDLDNIKKLELEISTVELQKLFIYLTNMEEK

Specific function: Part Of The Abc Transporter Complex Cysawtp Involved In Sulfate/Thiosulfate Import. Responsible For Energy Coupling To The Transport System. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27477115, Length=214, Percent_Identity=24.7663551401869, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI30795238, Length=214, Percent_Identity=28.5046728971963, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27881501, Length=214, Percent_Identity=28.5046728971963, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI21536376, Length=307, Percent_Identity=23.7785016286645, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI116734710, Length=226, Percent_Identity=27.8761061946903, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI31657092, Length=221, Percent_Identity=27.6018099547511, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI27436953, Length=218, Percent_Identity=23.8532110091743, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI6005701, Length=208, Percent_Identity=25.9615384615385, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI45446740, Length=216, Percent_Identity=27.7777777777778, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI47078218, Length=216, Percent_Identity=27.7777777777778, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI105990541, Length=213, Percent_Identity=25.8215962441315, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI153792144, Length=221, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI9955968, Length=229, Percent_Identity=26.2008733624454, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI9955966, Length=229, Percent_Identity=26.2008733624454, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI45243524, Length=229, Percent_Identity=26.2008733624454, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788761, Length=223, Percent_Identity=30.0448430493274, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081791, Length=210, Percent_Identity=27.6190476190476, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788450, Length=232, Percent_Identity=27.1551724137931, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48995001, Length=216, Percent_Identity=25.9259259259259, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790739, Length=227, Percent_Identity=22.0264317180617, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790190, Length=223, Percent_Identity=26.457399103139, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788210, Length=295, Percent_Identity=23.3898305084746, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786563, Length=218, Percent_Identity=27.5229357798165, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788225, Length=235, Percent_Identity=24.6808510638298, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI48994943, Length=227, Percent_Identity=22.4669603524229, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081709, Length=231, Percent_Identity=24.2424242424242, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787370, Length=218, Percent_Identity=25.6880733944954, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081782, Length=219, Percent_Identity=24.2009132420091, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787029, Length=219, Percent_Identity=27.8538812785388, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787792, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789586, Length=234, Percent_Identity=24.3589743589744, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786319, Length=293, Percent_Identity=26.6211604095563, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786345, Length=229, Percent_Identity=24.4541484716157, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788472, Length=235, Percent_Identity=26.3829787234043, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790467, Length=227, Percent_Identity=25.1101321585903, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787712, Length=217, Percent_Identity=27.1889400921659, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790162, Length=261, Percent_Identity=25.2873563218391, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788540, Length=191, Percent_Identity=29.8429319371728, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789891, Length=216, Percent_Identity=23.1481481481481, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790525, Length=236, Percent_Identity=23.728813559322, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789593, Length=232, Percent_Identity=25, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789672, Length=225, Percent_Identity=24, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788897, Length=217, Percent_Identity=22.5806451612903, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787143, Length=225, Percent_Identity=27.1111111111111, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI87082268, Length=218, Percent_Identity=24.7706422018349, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=228, Percent_Identity=28.5087719298246, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17552010, Length=263, Percent_Identity=25.8555133079848, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193211015, Length=221, Percent_Identity=29.8642533936652, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193211017, Length=221, Percent_Identity=29.8642533936652, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=222, Percent_Identity=28.3783783783784, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71983510, Length=222, Percent_Identity=28.3783783783784, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=222, Percent_Identity=28.3783783783784, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71996809, Length=221, Percent_Identity=29.8642533936652, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=201, Percent_Identity=26.865671641791, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=224, Percent_Identity=25.8928571428571, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322177, Length=202, Percent_Identity=26.2376237623762, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324585, Length=204, Percent_Identity=29.9019607843137, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650853, Length=235, Percent_Identity=29.7872340425532, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI281362751, Length=235, Percent_Identity=29.7872340425532, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24650855, Length=235, Percent_Identity=29.7872340425532, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582014, Length=227, Percent_Identity=27.7533039647577, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582012, Length=227, Percent_Identity=27.7533039647577, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI116007184, Length=224, Percent_Identity=27.2321428571429, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221500365, Length=224, Percent_Identity=27.2321428571429, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI85725262, Length=266, Percent_Identity=28.9473684210526, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24666092, Length=223, Percent_Identity=31.8385650224215, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221512771, Length=211, Percent_Identity=30.8056872037915, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24643674, Length=223, Percent_Identity=27.3542600896861, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI116007328, Length=215, Percent_Identity=30.2325581395349, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI28574150, Length=215, Percent_Identity=30.2325581395349, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28573571, Length=213, Percent_Identity=26.2910798122066, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573573, Length=212, Percent_Identity=26.4150943396226, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28571209, Length=213, Percent_Identity=24.8826291079812, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 33066; Mature: 32934

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEI
CCCEEEHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCHHHHHHHHHCCEEECCCCE
LIDGENVYENNKALSKVYFMEEKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSDLFKL
EECCCCCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHH
NIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLDANHRELFYKELLRIYGEGQK
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCE
TIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTI
EEEEEHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHEEECCCCCCHHHHEECCCCE
DIETLGSYKKATILQERTKEDLDNIKKLELEISTVELQKLFIYLTNMEEK
EHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHEEHHEEEECCCCC
>Mature Secondary Structure 
SNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEI
CCEEEHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCHHHHHHHHHCCEEECCCCE
LIDGENVYENNKALSKVYFMEEKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSDLFKL
EECCCCCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHH
NIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLDANHRELFYKELLRIYGEGQK
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCE
TIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTI
EEEEEHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHEEECCCCCCHHHHEECCCCE
DIETLGSYKKATILQERTKEDLDNIKKLELEISTVELQKLFIYLTNMEEK
EHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHEEHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]