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The map label for this gene is hgdB [H]

Identifier: 170754756

GI number: 170754756

Start: 2384211

End: 2385338

Strand: Reverse

Name: hgdB [H]

Synonym: CLD_2383

Alternate gene names: 170754756

Gene position: 2385338-2384211 (Counterclockwise)

Preceding gene: 170757665

Following gene: 170755992

Centisome position: 60.26

GC content: 30.23

Gene sequence:

>1128_bases
ATGGATAATATAAAAAATATTTTATCTAAATTAGAGGTAATTGTTAAGAACCCTAAAAAAGTTGTTTCAGATTATAAAGA
AAGAACAGGAAATAAAGTAATAGGATGTTTTCCAGTATATACTCCAGAGGAAATAGTATATGCAGCAGATATGCTTCCAA
TAGGTATTTGGGGAGGCGATGTTGAAGCTAATTTAGCTAAGCAATATTATCCAGCATTTTGTTGCTCTATAATGCAATCC
TGTATGGAGTTTGGCTTAAAAGGGGTATATGAGGGATTATCAGCTGTTATTATTCCAGGAATGTGTGACACATTAAATTG
TATGGGACAAAATTGGAAATTCGCCATTAAGGATATTCCATACATTGCATTAGTTCATCCTCAAAATAGAAAGTTAGAAG
CGGGCGTAGAGTATTTAGTTGAAGAATATAAACATGTAAAAGCAAAAATAGAAGAAATAAGAGGAAAAGAAATAACTGAA
GAAGAGCTACAAAATAGTATAGAAATATACAATGAACATAGAAAAGTAATGAGATCTTTTGTAGATGAAGCAGCTAAACA
TCCTAATACAATAAATAACTATCAAAGAAATCTTGTTATTAAAAGTGGATTCTTTATGAGAAAAGATGAACATACAAAAA
TAGTAAAAGAATTAAATGAGCTATTGAGTGTTTTACCAGAGGAAAAATATGATGGTAAAAAAGTATTAGTAACTGGAATA
CTTTTAGATTCAAAAGAAATGCTTGATGTTTTTGAAGAAAATAAATTAAGAATAGTAGCAGATGATTTAGCGCAGGAAAG
TAGACAATTTAGAACAGATGTACCAGAAGGAAAAGATGCATTAGATAGATTAGCAAGACAATGGTCAAATATTGAAGGAT
GTTCTTTGGCATATGATCCTAAAAAGATTAGAGGTTCAATGATTGCAAAGGAAGCTAAAGCTAAAGGAATAGATGGTGTA
GTATTTGCAATGATGAAATTCTGTGATCCAGAAGAATATGACTATCCAATTGTTAAAAAAGACATTGAAAAAGGAGACAT
TCCTACAACTATGATAGAAGTAGACCAACAAAACAAGAGTGTTGAACAAATAAGAACAAGAATTCAAACCTTCTCAGAAA
TATTATAG

Upstream 100 bases:

>100_bases
ACCAAGCAGATCCAAGAAACTTTAGTAAGGCTCAGTTTGAAACAAGACTGCAAGGCCTAGTAGAAGTTATGGAAGAAAGA
AAGAAAGGAGGAAACAAATA

Downstream 100 bases:

>100_bases
ACATAATTTCATACTAAGTTAGAATTTTAAATTTTGATGAAGGCTAGAAAATTATTATTTATTTAAATTTAATAAGCTGA
TTTCTAAGCTTAAGAGGGAG

Product: putative R-2-hydroxyglutaryl-CoA dehydratase, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MDNIKNILSKLEVIVKNPKKVVSDYKERTGNKVIGCFPVYTPEEIVYAADMLPIGIWGGDVEANLAKQYYPAFCCSIMQS
CMEFGLKGVYEGLSAVIIPGMCDTLNCMGQNWKFAIKDIPYIALVHPQNRKLEAGVEYLVEEYKHVKAKIEEIRGKEITE
EELQNSIEIYNEHRKVMRSFVDEAAKHPNTINNYQRNLVIKSGFFMRKDEHTKIVKELNELLSVLPEEKYDGKKVLVTGI
LLDSKEMLDVFEENKLRIVADDLAQESRQFRTDVPEGKDALDRLARQWSNIEGCSLAYDPKKIRGSMIAKEAKAKGIDGV
VFAMMKFCDPEEYDYPIVKKDIEKGDIPTTMIEVDQQNKSVEQIRTRIQTFSEIL

Sequences:

>Translated_375_residues
MDNIKNILSKLEVIVKNPKKVVSDYKERTGNKVIGCFPVYTPEEIVYAADMLPIGIWGGDVEANLAKQYYPAFCCSIMQS
CMEFGLKGVYEGLSAVIIPGMCDTLNCMGQNWKFAIKDIPYIALVHPQNRKLEAGVEYLVEEYKHVKAKIEEIRGKEITE
EELQNSIEIYNEHRKVMRSFVDEAAKHPNTINNYQRNLVIKSGFFMRKDEHTKIVKELNELLSVLPEEKYDGKKVLVTGI
LLDSKEMLDVFEENKLRIVADDLAQESRQFRTDVPEGKDALDRLARQWSNIEGCSLAYDPKKIRGSMIAKEAKAKGIDGV
VFAMMKFCDPEEYDYPIVKKDIEKGDIPTTMIEVDQQNKSVEQIRTRIQTFSEIL
>Mature_375_residues
MDNIKNILSKLEVIVKNPKKVVSDYKERTGNKVIGCFPVYTPEEIVYAADMLPIGIWGGDVEANLAKQYYPAFCCSIMQS
CMEFGLKGVYEGLSAVIIPGMCDTLNCMGQNWKFAIKDIPYIALVHPQNRKLEAGVEYLVEEYKHVKAKIEEIRGKEITE
EELQNSIEIYNEHRKVMRSFVDEAAKHPNTINNYQRNLVIKSGFFMRKDEHTKIVKELNELLSVLPEEKYDGKKVLVTGI
LLDSKEMLDVFEENKLRIVADDLAQESRQFRTDVPEGKDALDRLARQWSNIEGCSLAYDPKKIRGSMIAKEAKAKGIDGV
VFAMMKFCDPEEYDYPIVKKDIEKGDIPTTMIEVDQQNKSVEQIRTRIQTFSEIL

Specific function: Catalyzes the reversible dehydration of 2- hydroxyglutaryl-CoA to glutaconyl-CoA. This is a key step in the fermentation of glutamate [H]

COG id: COG1775

COG function: function code E; Benzoyl-CoA reductase/2-hydroxyglutaryl-CoA dehydratase subunit, BcrC/BadD/HgdB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082427, Length=368, Percent_Identity=23.6413043478261, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010327 [H]

Pfam domain/function: PF06050 HGD-D [H]

EC number: NA

Molecular weight: Translated: 42912; Mature: 42912

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNIKNILSKLEVIVKNPKKVVSDYKERTGNKVIGCFPVYTPEEIVYAADMLPIGIWGGD
CCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEECCC
VEANLAKQYYPAFCCSIMQSCMEFGLKGVYEGLSAVIIPGMCDTLNCMGQNWKFAIKDIP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCHHHHHHCCCCCEEEECCCC
YIALVHPQNRKLEAGVEYLVEEYKHVKAKIEEIRGKEITEEELQNSIEIYNEHRKVMRSF
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VDEAAKHPNTINNYQRNLVIKSGFFMRKDEHTKIVKELNELLSVLPEEKYDGKKVLVTGI
HHHHHHCCCHHHHHHHCEEEECCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
LLDSKEMLDVFEENKLRIVADDLAQESRQFRTDVPEGKDALDRLARQWSNIEGCSLAYDP
EECCHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCH
KKIRGSMIAKEAKAKGIDGVVFAMMKFCDPEEYDYPIVKKDIEKGDIPTTMIEVDQQNKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCH
VEQIRTRIQTFSEIL
HHHHHHHHHHHHHHC
>Mature Secondary Structure
MDNIKNILSKLEVIVKNPKKVVSDYKERTGNKVIGCFPVYTPEEIVYAADMLPIGIWGGD
CCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEECCC
VEANLAKQYYPAFCCSIMQSCMEFGLKGVYEGLSAVIIPGMCDTLNCMGQNWKFAIKDIP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCHHHHHHCCCCCEEEECCCC
YIALVHPQNRKLEAGVEYLVEEYKHVKAKIEEIRGKEITEEELQNSIEIYNEHRKVMRSF
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VDEAAKHPNTINNYQRNLVIKSGFFMRKDEHTKIVKELNELLSVLPEEKYDGKKVLVTGI
HHHHHHCCCHHHHHHHCEEEECCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
LLDSKEMLDVFEENKLRIVADDLAQESRQFRTDVPEGKDALDRLARQWSNIEGCSLAYDP
EECCHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCH
KKIRGSMIAKEAKAKGIDGVVFAMMKFCDPEEYDYPIVKKDIEKGDIPTTMIEVDQQNKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCH
VEQIRTRIQTFSEIL
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2659350; 3691501; 11106419 [H]