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The map label for this gene is cobI

Identifier: 170754705

GI number: 170754705

Start: 1058985

End: 1059701

Strand: Direct

Name: cobI

Synonym: CLD_3614

Alternate gene names: 170754705

Gene position: 1058985-1059701 (Clockwise)

Preceding gene: 170757374

Following gene: 170755796

Centisome position: 26.75

GC content: 29.15

Gene sequence:

>717_bases
ATGAAATATATACATAATAATATAGAATTTGAAAATAGGGATCCAGATAAAAAATACGGAACCTTATATGGAATTGGAGT
AGGTCCAGGAAATGAAGAGTTATTAACTATAAAAGCTGTAAAAGTTTTAGAAAGCTGTGATGCCGTTATAGCTCCAACAG
CAAGAGAAAATGGGGAAAGCATAGCTCTAAATACAGCTAGAAATTTTATAAACCCTAAAGCAGAAATATATTTAAAATAT
TTTCCGATGAAAAAGGAAAAGGAAGCCGAGATATATGAGAACTATAGATTTATGGAAAAATTATTATCAGAAGGAAAAAA
TGTAGCATTTCTAACTATAGGAGACCCTTTTGTATATAGTACTTATATATATTTATTAGAATATATGAGAAATCATAATT
TAAATATAGAAACAGTACCAGGCATAACTTCTTTTTGTGCAGCAGCAAGTTTAGCAGGGGAAACTTTAGTTATAGGAGAT
GAACCACTTTTAATATTGCCAGGGAATAGGTTAGATTCTATAAAGGATGAAAAGTACTTAGTAATAATGAAATATTATAA
AAATGTAAAACAGGTTTTAGACAAGCTAGAAGAAAAGGGTTTTAAATATATATGCGTAAAAAGAGCTTATAGAGAGGGGC
AAGAAATCTTAAGAACAAGAGAAGAAATCCTGAATTCTAAAGATTATATGTCAATTATAATAGCCAATAGGGAATAG

Upstream 100 bases:

>100_bases
GGCGGATTAAGGGTTTAAGACATAATTTTTTATAGATAGTAAATACTTAAAATTAATTTATATGAAGAAATATAAATCAT
TGATATTAAGGAGTTAACGC

Downstream 100 bases:

>100_bases
AATATATTTATTAATTTAATAAGATAATTTATCTTATTAAGGCTGTAGAACATATTTATCAATCTTATAAGAAAGTTTAT
ATTATTTAATGAAGTGGTAA

Product: cobalt-precorrin-2 C(20)-methyltransferase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKYIHNNIEFENRDPDKKYGTLYGIGVGPGNEELLTIKAVKVLESCDAVIAPTARENGESIALNTARNFINPKAEIYLKY
FPMKKEKEAEIYENYRFMEKLLSEGKNVAFLTIGDPFVYSTYIYLLEYMRNHNLNIETVPGITSFCAAASLAGETLVIGD
EPLLILPGNRLDSIKDEKYLVIMKYYKNVKQVLDKLEEKGFKYICVKRAYREGQEILRTREEILNSKDYMSIIIANRE

Sequences:

>Translated_238_residues
MKYIHNNIEFENRDPDKKYGTLYGIGVGPGNEELLTIKAVKVLESCDAVIAPTARENGESIALNTARNFINPKAEIYLKY
FPMKKEKEAEIYENYRFMEKLLSEGKNVAFLTIGDPFVYSTYIYLLEYMRNHNLNIETVPGITSFCAAASLAGETLVIGD
EPLLILPGNRLDSIKDEKYLVIMKYYKNVKQVLDKLEEKGFKYICVKRAYREGQEILRTREEILNSKDYMSIIIANRE
>Mature_238_residues
MKYIHNNIEFENRDPDKKYGTLYGIGVGPGNEELLTIKAVKVLESCDAVIAPTARENGESIALNTARNFINPKAEIYLKY
FPMKKEKEAEIYENYRFMEKLLSEGKNVAFLTIGDPFVYSTYIYLLEYMRNHNLNIETVPGITSFCAAASLAGETLVIGD
EPLLILPGNRLDSIKDEKYLVIMKYYKNVKQVLDKLEEKGFKYICVKRAYREGQEILRTREEILNSKDYMSIIIANRE

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.151 [H]

Molecular weight: Translated: 27396; Mature: 27396

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIHNNIEFENRDPDKKYGTLYGIGVGPGNEELLTIKAVKVLESCDAVIAPTARENGES
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCCEECCCCCCCCCE
IALNTARNFINPKAEIYLKYFPMKKEKEAEIYENYRFMEKLLSEGKNVAFLTIGDPFVYS
EEEEHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
TYIYLLEYMRNHNLNIETVPGITSFCAAASLAGETLVIGDEPLLILPGNRLDSIKDEKYL
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCEE
VIMKYYKNVKQVLDKLEEKGFKYICVKRAYREGQEILRTREEILNSKDYMSIIIANRE
EHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
>Mature Secondary Structure
MKYIHNNIEFENRDPDKKYGTLYGIGVGPGNEELLTIKAVKVLESCDAVIAPTARENGES
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCCEECCCCCCCCCE
IALNTARNFINPKAEIYLKYFPMKKEKEAEIYENYRFMEKLLSEGKNVAFLTIGDPFVYS
EEEEHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
TYIYLLEYMRNHNLNIETVPGITSFCAAASLAGETLVIGDEPLLILPGNRLDSIKDEKYL
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCEE
VIMKYYKNVKQVLDKLEEKGFKYICVKRAYREGQEILRTREEILNSKDYMSIIIANRE
EHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]