| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is nrdA
Identifier: 170754701
GI number: 170754701
Start: 2999252
End: 3001543
Strand: Reverse
Name: nrdA
Synonym: CLD_1760
Alternate gene names: 170754701
Gene position: 3001543-2999252 (Counterclockwise)
Preceding gene: 170755759
Following gene: 170756361
Centisome position: 75.83
GC content: 31.15
Gene sequence:
>2292_bases ATGAATATTAAAATAAAAAAGAGAGATGGACAATATGAACCACTACAAGTAGAAAAAACAAAGAAAATGGTTAAGTTAGC CTGTGAGGGTATAGAAGGATGTGACCCTCTTGAACTAGAGTTGGATTCCAGAATACAATTTAGAGATGGTATGACTACTA AGGAAATTCAAAGAACTTTGATACAAACTGCCATCGAAAAAGTTATTCAAAATAGCAAAGATAACAATGGAAATAATATA AAAAAAACTAATGCTAATTGGCAGTATGTTGCTGCAAGACTTTTATGTTTTGATTTATACAAGGAAGCTAAAATCAGCCG ACACTATAATAGCTTTGGTTATGGTGATTATTATGAATTGGTAAAGAAATTAGTTAAAATTAAACTTTATGGAGAGTATT TAATTCAAAATTATTCTGATGAAGAAATCAAAGAATTAGCAAAGTATATAGTACCAGAAAGAGATGAATTATTTAATTAT GAAGGATTAAAATTATTAAATGATAGATATTTAATTAAGGGGCATAATGGAGAAATATTAGAGCTTCCTCAAGAACGCTT TATGACAATAGCTATGCATTTAGCTATTCCTGAGGGGGATAAAAAAGTTTTTTATGCAAAAAAGTTTTATGATATTTTAA GTGAGTTAAAAGTTACAGTGGCCACTCCTACTTTAGGAAATGCAGGTACACCTTTTTATCAGTTAAGTAGCTGTTTTATA TCTGTTGTAGGGGATAATTTATGGTCAATCTATGATGTTAATCAAAAGTTTGCGCAAGTCTCAAAACATGGTGGAGCACT AGGTATTTATACTGGGAAAATAAGAGCGTTAAACAGTGAAATAAGAGGACATAAAAATGCATCAGGGGGGGTTGTACCTT GGATAAGACTTTATAATGATACAGCTGTAGCTGTAGACCAGCTTGGCAAGAGAAAAGGTGGAGCAGCTATAACTTTAGAT ATATGGCATAAAGATATATTTGATTTTTTAGATCTTAAAACTAATAATGGAGATGATAGAAGAAAGGCTCATGATATTTT TCCTTCTGTAAGCATTCCAGACTTATTCATGAAAAGATTAGAGAAAAGAGAAAGTTGGTCTTTATTTGATCCTTATATTG TAGAGAAGATAATGGGCTACAAGTTGGAGGATTATTTTGATGATGAAGATCGCAGGGAATTTACAAATAAGTACTTAGAA TGTGAAAGAAATACTAATATTCCAAGAGATACAGTACCGACTCTTGATATTATGAAAAAATTAATGAAAAGTGCAGTAGA GACAGGTACTCCTTTTATTTTTTTTAGGGATACTGTAAATAAAGCAAATCCTAATAAACATAAAGGTATGATATATTCAT CAAATCTTTGCCATGAAATAGCTCAAAATATGAGTGAAAGTCGACTTCTAGAAGAGGAAATAATTGATGGTAATGGTTAC TCAGAGATTGTTCAAAGAGTTAAGGCTGGAGATATGGTAACCTGTAATCTTAATTCAATAAATCTTAGCAAAGTGAAAAA AGAGGAATTTAATGAGTGTATCCCTTTTCAAATAAGGATGCTAGATAATGTTATATCTCTTAATAAGCTTCCTGTTAAAG AGGCAAAAGTTACCAGTGACAAGTATAGAGCTATTGGTTTAGGAACAAGTGGATATCATCATTTCCTTGCAAATAACAAG ATAAGATGGGAAAGTGATGAACATATTAAGGTAGCGGATGAAATCTATGAAGAGATAGCCTATATAGCTATAAAATCTTC CATGGAATTAGCTAAGGAAAAAGGCAGTTATCCAGCTTTTAAGGATTCAGAATGGGAAACAGGAAAATATTTTGAAAGAA GAGGTTACAATTCTGAAAGATGGAAGAAGCTACAAAGTAATATCAAAAAATATGGTATGAGAAATGGTTATATTACTGCT ATTGCCCCTACAGGAAGTACTTCTAATATAGCTAATACAACAGCAGGAATAGACCCTGTATTTAAAAAGTTTTTTATGGA AGAAAAAAAGGGTAGCTTTACACCAAAAACAGCACCAGACTTAAATGAAGAAAATTTTTGGTATTATAAAGAAGCACATA CTATAGACCAACAATGGAGCATTAAGGCTTGTGCTGTAAGACAAAAGCATATAGACCAGGCTCAATCCTTTAATTTATAT ATAACTCCAGAAATAAAAGCTAAAGAAATTCTTAATATGTATATGGAATCTTGGAAGCAAGGAGTTAAAACTATCTATTA TGTAAGAAATAAATCTTTAGAAATGGATGAATGTACAAGTTGCTCAAGCTAA
Upstream 100 bases:
>100_bases TTGATTTTAAAAATAATAACTTATATAATTTATTTAACAACGGTTATTTTATAATATTTTTTATTGATTAAATAAATTTT TAGAAAGTAAGGTTTGAGCA
Downstream 100 bases:
>100_bases ATGTATATTTAAAGTTATAACAAGATAAAGATTGTAATTTTAAAGCAGATGAAGTGCTAGGGTTAAAGTATATACTTAAT ATAATACCTAAATGGGTAAA
Product: ribonucleotide-diphosphate reductase subunit alpha
Products: NA
Alternate protein names: Ribonucleotide reductase [H]
Number of amino acids: Translated: 763; Mature: 763
Protein sequence:
>763_residues MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTLIQTAIEKVIQNSKDNNGNNI KKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYELVKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNY EGLKLLNDRYLIKGHNGEILELPQERFMTIAMHLAIPEGDKKVFYAKKFYDILSELKVTVATPTLGNAGTPFYQLSSCFI SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYNDTAVAVDQLGKRKGGAAITLD IWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRLEKRESWSLFDPYIVEKIMGYKLEDYFDDEDRREFTNKYLE CERNTNIPRDTVPTLDIMKKLMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSDKYRAIGLGTSGYHHFLANNK IRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAFKDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITA IAPTGSTSNIANTTAGIDPVFKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY ITPEIKAKEILNMYMESWKQGVKTIYYVRNKSLEMDECTSCSS
Sequences:
>Translated_763_residues MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTLIQTAIEKVIQNSKDNNGNNI KKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYELVKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNY EGLKLLNDRYLIKGHNGEILELPQERFMTIAMHLAIPEGDKKVFYAKKFYDILSELKVTVATPTLGNAGTPFYQLSSCFI SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYNDTAVAVDQLGKRKGGAAITLD IWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRLEKRESWSLFDPYIVEKIMGYKLEDYFDDEDRREFTNKYLE CERNTNIPRDTVPTLDIMKKLMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSDKYRAIGLGTSGYHHFLANNK IRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAFKDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITA IAPTGSTSNIANTTAGIDPVFKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY ITPEIKAKEILNMYMESWKQGVKTIYYVRNKSLEMDECTSCSS >Mature_763_residues MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTLIQTAIEKVIQNSKDNNGNNI KKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYELVKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNY EGLKLLNDRYLIKGHNGEILELPQERFMTIAMHLAIPEGDKKVFYAKKFYDILSELKVTVATPTLGNAGTPFYQLSSCFI SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYNDTAVAVDQLGKRKGGAAITLD IWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRLEKRESWSLFDPYIVEKIMGYKLEDYFDDEDRREFTNKYLE CERNTNIPRDTVPTLDIMKKLMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSDKYRAIGLGTSGYHHFLANNK IRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAFKDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITA IAPTGSTSNIANTTAGIDPVFKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY ITPEIKAKEILNMYMESWKQGVKTIYYVRNKSLEMDECTSCSS
Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]
COG id: COG0209
COG function: function code F; Ribonucleotide reductase, alpha subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ATP-cone domain [H]
Homologues:
Organism=Homo sapiens, GI4506749, Length=806, Percent_Identity=29.9007444168734, Blast_Score=306, Evalue=4e-83, Organism=Escherichia coli, GI2367133, Length=768, Percent_Identity=27.8645833333333, Blast_Score=303, Evalue=3e-83, Organism=Escherichia coli, GI1789030, Length=696, Percent_Identity=27.7298850574713, Blast_Score=259, Evalue=5e-70, Organism=Caenorhabditis elegans, GI17555242, Length=796, Percent_Identity=29.2713567839196, Blast_Score=294, Evalue=1e-79, Organism=Saccharomyces cerevisiae, GI6320914, Length=801, Percent_Identity=28.7141073657928, Blast_Score=299, Evalue=1e-81, Organism=Saccharomyces cerevisiae, GI6322123, Length=805, Percent_Identity=29.4409937888199, Blast_Score=291, Evalue=2e-79, Organism=Drosophila melanogaster, GI17352481, Length=796, Percent_Identity=29.2713567839196, Blast_Score=299, Evalue=5e-81,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005144 - InterPro: IPR013346 - InterPro: IPR013509 - InterPro: IPR000788 - InterPro: IPR008926 [H]
Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]
EC number: =1.17.4.1 [H]
Molecular weight: Translated: 88115; Mature: 88115
Theoretical pI: Translated: 8.27; Mature: 8.27
Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTL CCEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCHHHHHHHH IQTAIEKVIQNSKDNNGNNIKKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYEL HHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH VKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNYEGLKLLNDRYLIKGHNGEIL HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCEEEECCEEEEECCCCCEE ELPQERFMTIAMHLAIPEGDKKVFYAKKFYDILSELKVTVATPTLGNAGTPFYQLSSCFI ECCHHHHHEEEEEEECCCCCCEEEHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHH SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYND HHHCCCCEEEEEHHHHHHHHHHCCCEEEEEECHHEEHHHHHCCCCCCCCCCCEEEEEECC TAVAVDQLGKRKGGAAITLDIWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRL CHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHH EKRESWSLFDPYIVEKIMGYKLEDYFDDEDRREFTNKYLECERNTNIPRDTVPTLDIMKK HHCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH LMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCCCH SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSD HHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCC KYRAIGLGTSGYHHFLANNKIRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAF CEEEEECCCCCHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC KDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITAIAPTGSTSNIANTTAGIDPV CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHH FKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY HHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEE ITPEIKAKEILNMYMESWKQGVKTIYYVRNKSLEMDECTSCSS ECCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHCCCCC >Mature Secondary Structure MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTL CCEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCHHHHHHHH IQTAIEKVIQNSKDNNGNNIKKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYEL HHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH VKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNYEGLKLLNDRYLIKGHNGEIL HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCEEEECCEEEEECCCCCEE ELPQERFMTIAMHLAIPEGDKKVFYAKKFYDILSELKVTVATPTLGNAGTPFYQLSSCFI ECCHHHHHEEEEEEECCCCCCEEEHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHH SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYND HHHCCCCEEEEEHHHHHHHHHHCCCEEEEEECHHEEHHHHHCCCCCCCCCCCEEEEEECC TAVAVDQLGKRKGGAAITLDIWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRL CHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHH EKRESWSLFDPYIVEKIMGYKLEDYFDDEDRREFTNKYLECERNTNIPRDTVPTLDIMKK HHCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH LMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCCCH SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSD HHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCC KYRAIGLGTSGYHHFLANNKIRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAF CEEEEECCCCCHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC KDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITAIAPTGSTSNIANTTAGIDPV CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHH FKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY HHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEE ITPEIKAKEILNMYMESWKQGVKTIYYVRNKSLEMDECTSCSS ECCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9537320 [H]